Chem 8quiz bank Chem quiz bank ch 8 1. All of the following statements are true except a.

In an RNA DNA hybrid, adenine binds to thymine with three hydrogen bonds[FALSE] b. Cytosine binds to guanine with 3 hydrogen bonds c. In an RNA-DNA hybrid adenine binds with uracil with 2 hydrogen bonds d. Adenine binds to thymine with 2 hydrogen bonds 2. All of the following are required for a PCR reaction EXCEPT a. Restriction endonuclease [not needed] b. Oligonucleotide primers [necesito] c. Thermostable DNA polymerase [necesito] d. Individual deoxyribonucleotides [necesito] e. Target DNA [necesito] 3. Which of technique used endonucleases? a. PCR [NOPE] b. Ligase chain reaction[NOPE] c. Transcription-based amplification [NOPE] d. Southern-blot [yes] 4. Match the method with the appropriate description a. Northern blot messenger RNA b. Southern blot- single stranded DNA c. Western blot proteins 5. Match the technique with the appropriate amplification a. Ligase chain reaction-probe amplification b. Polymerase chain reaction-target amplification c. Signal amplification branched chain DNA assay 6. T/F the overall charge of a DNA molecule is negative [true] 7. The nucleic acid technique in which RNA is converted to cDNA which is then amplified in known as [RT-PCR]