To: BioSphere

Eden Research Laboratory

Update

Date: January 25, 2013

From: Thomas Poth

Number of pages including cover: 2
Regarding: ASTM D 5511-11 test update for PET Clear Food Containers - 47 days

2111 Menaul NE, Suite A

Albuquerque, NM 87107
T 505.508.3994

Inculum
Cumulative Gas
Volume (mL)
Percent CH4 (%)
Volume CH4
(mL)
Mass CH4 (g)
Percent CO2 (%)
Volume CO2
(mL)
Mass CO2 (g)
Sample Mass (g)
Theoretical
Sample Mass (g)
Biodegraded
Mass (g)
Percent Biodegraded (%)
* Adjusted Percent Biodegraded (%)

Negative

Positive

1386.7
18.7

1119.8
35.5

10070.6
39.4

6937.9
36.4

259.2
0.19
49.7

397.0
0.28
40.8

3967.5
2.83
42.5

2524.2
1.80
42.7

689.5
1.35
1,000

457.0
0.90
10

4279.8
8.41
10

2963.1
5.82
20.0

0.0

8.6

4.4

12.4

0.51

0.46

4.42

2.94

-0.6

88.5

19.6

-0.7

100.0

22.2

Biodegradation
90.0
67.5
Percent (%)

M 505.550.9316
thomas.poth@edenresearchlab.com

634 - Clear
Plastic Container

45.0
22.5
0
-22.5

13

25

38

Time (days)
NEG

POS

634A-634 - Clear Plastic Container

50

Biodegradation
Eden Research Laboratory

20.0

Update
M 505.550.9316
thomas.poth@edenresearchlab.com

Percent (%)

2111 Menaul NE, Suite A

Albuquerque, NM 87107
T 505.508.3994

15.0

10.0

5.0

-5.0

13

25

38

50

Time (days)
NEG

634A-634 - Clear Plastic Container

* Outside of the ASTM D5511 method and on the assumption that the positive control (Cellulose) will fully biodegrade, all value have been proportionally adjusted.