Pour gels

1. Make sure that the gel tray is sealed with the blocks or with 2-3 pieces of tape
2. Place 1-2 combs in the gel
3. Pour liquid molten agarose into the gels up to just below the top of the comb teeth
4. Let cool

Extract “DNA” and cut with restriction enzymes

1. Place “DNA” samples in labeled test tubes with 1 mL of hot water
2. Agitate
3. Place in boiling water bath to concentrate the sample

Loading the Gels

1. Gently remove the combs
2. Set the micropipette to 20 uL.
3. Using a new sterile tip for each well place 20 uL of sample going from left to
right. Label which wells get which sample below. Everyone should practice
loading a few wells

Run the Gels

1. Set the gel tray near the power supply
2. Pour running buffer into the tray
3. Gently place the gel into the tray with the samples near the ________________
end (because DNA is ______________)
4. Plug in the tray. Check for bubbles on the electrodes (this shows current is
flowing and causing electrolysis of water)
5. Let it run for about 30 minutes and check to see if the bands have separated
enough

Gel Diagram