TRNG I HC NHA TRANG

KHOA CNG NGH THC PHM

HNG DN X L S LIU THC NGHIM BNG SPSS

1. S KHC BIT C NGHA THNG K GIA CC GI TR TRUNG BNH 2. PHN TCH HI QUY

Ngi trnh by: Nguyn Vn Tng

Nha Trang - 4/2013

VN T RA Th tc x l thng k cn p dng l g? Phn mm h tr nh th no? Thao tc x l ra sao? Cch c kt qu nh th no?

1. S KHC BIT C NGHA THNG K GIA CC GI TR TRUNG BNH Khi biu din kt qu th nghim Thng tin cn c trn bng, biu gm: - Gi tr trung bnh - lch chun - K hiu ch c hay khng s khc bit c ngha thng k (p<0,05) gia cc gi tr trung bnh (thng dng ch ci a,b,c)

Bng 1-Nng sut sy rong nho ti, nng sut chit v hm lng phenolic tng s ca rong nho sy nhit, sy lnh ng v ch long Hm lng Nng sut sy (g/100 g nguyn Nng sut chit phenolic tng s (g/100 g cht (mg GAE*/g liu ti) kh) cht kh) 2.21 0.08a** 7.62 0.11c 1.30 0.02c 2.26 0.09a 11.02 0.05b 2.04 0.03b 15.99 0.09a 13.58 0.01a nd

Loi nguyn liu Rong nho sy nhit Rong nho sy lnh ng Ch long

*GAE, Gallic acid equivalent; nd, Khng xc nh

Ngun: Nguyn v cng s (2011)

100 90 Hiu qu kh g c t do DPPH (%). 80 70 60 50 40 30 a 20 10 c 0 20 40 60 N ng dch chit (ppm) 80 100 c b c c c c c c c c a b

Vitamin C C Vitamin Rong nho swith y nhitthermal-drying Seagrape Rong nho sy lnh freeze-drying ng Seagrape with Ch long Oolong tea

a b

a b

Hnh 1-Kh nng kh gc t do DPPH ca dch chit rong nho sy nhit v sy lnh ng so snh vi ch long v vitamin C (cht chun).
Ngun: Nguyn v cng s (2011)

BI TP TNH HUNG
Kho st nh hng ca nng ethanol n hm lng polyphenol trong dch chit ch en Mc tiu cn gii quyt: - C s khc bit c ngha thng k v hm lng polyphenol trong dch chit ch en cc nng ethanol khc nhau hay khng?

S liu thc nghim

Nng Ethanol 50 60 70 80 90 99,5 TPC (ppm) Ln 1 419.730 507.152 524.320 602.112 614.528 222.870 Ln 2 399.636 480.356 528.264 617.600 619.520 242.604

Ngun: Trn v Nguyn (2013)

 Th tc x l thng k cn p dng:
- Phn tch phng sai 1 yu t - Yu t: Nng ethanol vi s mc k = 6 (tng ng vi cc nng : 50; 60; 70; 80; 90; v 99,5%) - Gi tr quan st: Hm lng polyphenol (ppm)

 Phn mm SPSS h tr x l: 1. Dng One way ANOVA ch ra c hay khng s khc bit c ngha thng k gia cc gi tr trung bnh 2. Dng One way ANOVA (Options Homogeneity of variance test ) ch ra c hay khng s ng nht gia cc phng sai ca cc nhm l c s chn test trong Post Hoc Multiple Comparisions

 Phn mm SPSS h tr x l: 3. Dng One way ANOVA (Post Hoc Multiple Comparisions) ch ra c th s khc bit c ngha thng k gia cp gi tr trung bnh no v nng ethanol l bao nhiu th hm lng polyphenol trong dch chit ch en l cao (thp) nht

 Thao tc x l
M giao din SPSS Khai bo tn bin: chn Variable View khai bo tn bin theo hng

 Thao tc x l
Khai bo d liu ca bin: chn Data View khai bo d liu ca bin theo ct

 Thao tc x l
Thc hin phn tch d liu: Chn Analyze Compare Means One way ANOVA

 Thao tc x l
Trong hp thoi khai bo yu t v bin ph thuc (gi tr quan st)

 Thao tc x l
Chn option nh du vo: - Homogeneity of variance test bit phng sai ca cc nhm c ng nht hay khng

 Cch c kt qu
Nhn vo gi tr Sig trong bng ANOVA bit c hay khng s khc bit cc gi tr trung bnh: Sig (0,05): c s khc bit tip tc phn tch su ANOVA

 Cch c kt qu
- Nhn vo gi Sig trong bng Test Homogeneity bit c hay khng s khc bit v phng sai gia cc nhm.

 Cch c kt qu
- Sig : khng c s khc bit v phng sai Trong Post Hoc Multiple Comparisions chn nhm test (LSD; Duncan; Scheff; Tukey; Bonferroni; ) cho phn phn tch s khc bit gia cc gi tr trung bnh.

 Cch c kt qu
- Sig : c s khc bit v phng sai Trong Post Hoc Multiple Comparisions chn nhm test (Tamhanes T2; Dunnnetts T3; Dunnnetts C) cho phn phn tch s khc bit gia cc gi tr trung bnh.

 Cch c kt qu
Vi tnh hung ny gi tr sig trong bng ANOVA v Test Homogeneity u < 0,05, nn trong Post Hoc Multiple Comparisions chn test Dunnnetts T3 cho phn phn tch s khc bit gia cc gi tr trung bnh Kt qu th hin trong bng sau

 Cch c kt qu
Kt qu th hin trong hp Post Hoc Test: Du * th hin s khc bit gia cc cp gi tr trung bnh Khi khi trnh by s liu trong biu ta s dng cc ch ci a,b,c phn bit s khc nhau c ngha thng k gia cc cp gi tr trung bnh
Nng Ethanol TPC 50 b 60 bc 70 c 80 d 90 d 99,5 a

2. PHN TCH HI QUY

Cho php d on din bin ca qu trnh da vo phng trnh hi quy v h s xc nh (R2)
hp th 760 nm 1,000 0,800 0,600 0,400 0,200 0,000 0 10 20 30 40 50 y = 0,015x + 0,1072 R2 = 0,9957

Nng axit galic (ppm)

Ngun: Trn v Nguyn (2013)

 Th tc x l cn p dng:
- Phn tch hi quy tuyn tnh: Phng trnh tuyn tnh: y = ax + b - Bin c lp: nng ethanol; bin ph thuc: hm lng polyphenol

 Phn mm SPSS h tr x l: 1. Dng Regression - Linear 2. Dng Regression - Curve estimation

 Thao tc x l
1. Chn Analyze Regression Linear 2. Chn Analyze Regression curve estimation Khai bo tn bin: + Independent: nongdoETHANOL + Dependent: TPC (Model: Chn linear)

 Cch c kt qu

 Cch c kt qu

Phng trnh hi quy: y = -0,995x + 548.610 H s xc inh: R2 = 0.015

 Cch c kt qu

Chn thnh cm n