ABSTRACT Spectrophotometric analytical methods use light and other forms of electromagnetic radiation to make measurements.

In particular, the amount of radiation absorbed or produced by a molecular or atomic species of interest is measured and classified according to the region of the electromagnetic spectrum involved in the measurement. Nitrite in natural waters can be determined spectrophotometrically or other methods including IR, fluorimetric methods and through titration. By Canadian drinking water guidelines, the recommended nitrite concentration in safe drinking water should not exceed 3.2 mg/L. This experiment studies the quantitative determination of nitrite in a particular freshwater sample. This is achieved with spectrophotometric methods, in which the concentration of nitrite is determined by taking the samples light absorbance and at various concentrations of nitrite set in a standard analyte solution. The sample analyte concentration is determined to be 49.5 g/L, which is accepted by Canadian drinking water guidelines. This is determined by comparing its light transmission to the calibration curve. The incident light used in this experiment follows = 543 nm.