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Collecting Embryos Fairly newly emerged flies if possible (not super old).

Then put the in new bottle of food overnight (12 hours to over weekend). Good nutrition and enough space. Then place in cage overnight (at least 12 hours x). Collect 2-4 hours. (If laying really well, only need 2 hour collections, but if not laying well, then need longer collection time.) Age them at RT or 25 degrees Celsius for 8hrs: If 2 hour collection, then 8 hours aging = 8-10 hours If 4 hour collection, then 8 hours aging = 8-12 hours. Then place at 4 degrees till uses: -sorting -embryo fix See when twist comes on wall posting.

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