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8.2 Studying the effects of temperature on the activity of yeast Problem statement: How does the temperature affect the activity of yeast? Hypothesis: The activity of yeast is optimal at temperature of 35 oC. Variables: Manipulated Variable Temperature Responding Variable Height of coloured liquid in the manometer Constant Variable Volume of yeast, time taken Materials and apparatus: Yeast suspension, coloured liquid, paraffin oil, boiling tube, glass tubes, clips, rubber stoppers, rubber tubings, retort stands, manometer tubes, strings, measuring cylinder, stopwatches and thermometers Procedure:

(P1) Diagram 1 1. 2. 3. 4. Label five boiling tubes A, B, C, D and E. (P1) Fill the boiling tubes with 15 ml of yeast suspension. (P1, P2) Set up five apparatus set up as shown in the Diagram 1. (P1) Place each boiling tube into water bath with different temperature as below: Bioling tube A 0 oC

Imelda anak Nyaun/S.M.K. Serian

Boiling tube B 20 oC Boiling tube C 35 oC Boiling tube D 40 oC Boiling tube E 60 oC (P4) 5. Start the stopwatch immediately and record the height of coloured liquid in the manometer after 10 minutes. (P2, P5) 6. Record the result in a table. (P3) Result: Boiling tube A B C D E Temperature (oC) 0 20 35 40 60 Height of coloured liquid in the manometer (cm)

Imelda anak Nyaun/S.M.K. Serian