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2 UNIT TWO: THE CELL Chapter Eight: Membrane Structure and Function (Text from Biology, 6th Edition, by Campbell and Reece) A membrane remains fluid as temperature decreases until the phospholipids settle into a closely packed arrangement and the membrane solidifies. The steroid cholesterol, wedged between phospholipid molecules in the plasma membranes of animal cells, has different effects on membrane fluidity. At human body temperature, it restrains phospholipid movement. However, it also lowers the temperature required for solidification. Membranes must be fluid to work properly. Membranes Are Mosaics of Structure and Function There are two major populations of membrane proteins. Integral proteins penetrate the hydrophobic core of the lipid bilayer. Many of them are transmembrane proteins, which means they completely cross the membrane. The hydrophobic regions of an integral protein consist of one or more stretches of nonpolar amino acids, usually coiled into helices. The hydrophilic parts are exposed to the aqueous solution on either side of the membrane. Peripheral proteins are not embedded in the lipid bilayer, but are loosely bound to the surface of the membrane. Often, they are bound to the exposed parts of integral proteins. Some membrane proteins on the cytoplasmic side of the plasma membrane are held in place by attachment to the cytoskeleton. On the exterior side, certain membrane proteins are attached to fibers of the ECM. These attachments combine to give animal cells a strong external framework. Membranes have distinct inside and outside faces. The two lipid layers may differ in specific lipid composition, and each protein has directional orientation in the membrane. Carbohydrates are only found on the exterior surface. Membrane Carbohydrates Are Important For Cell-Cell Recognition Cells can recognize other cells by keying on surface molecules, often carbohydrates, on the plasma membrane. Membrane carbohydrates are usually branched oligosaccharides with fewer than 15 sugar units. Some of these oligosaccharides are covalently bonded to lipids, which forms molecules called glycolipids. Most are covalently bonded to proteins (glycoproteins). Oligosaccharides on the external side of the plasma membrane vary from species to species, individuals of the same species, and even from one cell type to another.
3 UNIT TWO: THE CELL Chapter Eight: Membrane Structure and Function (Text from Biology, 6th Edition, by Campbell and Reece) TRAFFIC ACROSS MEMBRANES A Membranes Molecular Organization Results in Selective Permeability
Transport Proteins
Transport proteins that span the membrane can provide channels through which hydrophilic substances can cross. These transport proteins are highly specific. Passive Transport is Diffusion Across a Membrane Molecules have intrinsic kinetic energy called heat. One result of thermal motion is diffusion diff usion, usion the tendency for molecules to spread out into available space. Although molecules themselves move randomly, diffusion of a population may be directional. A substance will diffuse from where it is more concentrated to where it is less concentrated: substances diffuse down their concentration gradient. gradient This is a spontaneous process because it decreases the free energy in a system by increasing entropy (disorder). Diffusion of substances across a biological membrane is passive transport because the cell does not have to expend energy to make it happen. The concentration gradient itself is potential energy and drives diffusion.
4 UNIT TWO: THE CELL Chapter Eight: Membrane Structure and Function (Text from Biology, 6th Edition, by Campbell and Reece) Osmosis is the Passive Transport of Water In comparing two solutions of unequal solute concentration, the solution with higher concentration of solute is said to be hypertonic. hypertonic Conversely, the solution with lower solute concentration is hypotonic. hypotonic These terms only make sense used comparatively. Solutions of equal solute concentration are isotonic. isotonic The direction of osmosis, osmosis the diffusion of water, is determined by a difference in total solute concentration. Water moves from a hypotonic to a hypertonic solution. It is helpful to remember that were there is more solute, there is less water and where there is less solute, there is more water; thus, water moves from areas of less solute to more solute. When two solutions are isotonic, there is still movement, but it is at an equal rate, so there is no net movement. Cell Survival Depends on Balancing Water Uptake and Loss
5 UNIT TWO: THE CELL Chapter Eight: Membrane Structure and Function (Text from Biology, 6th Edition, by Campbell and Reece) resemble the normal passenger and binds to the protein. Unlike enzymes, proteins catalyze a physical
process. Channel proteins allow water molecules or small ions to flow quickly from one side of the membrane to
the other. Water channel proteins are aquaporins. aquaporins Some channel proteins are gated channels; channels stimuli causes the gate to pen or close. Other proteins can change shape to translocate the solute-binding site across the membrane. Active Transport is the Pumping of Solutes Against Their Gradient Active transport requires work, since a molecule is being pumped across a membrane against its gradient. This is a major factor in the ability of a cell to maintain internal concentrations of small molecules that differ from concentrations in its environment. ATP supplies the energy for most active transport. This can occur through phosphorylation. In the sodiumsodium - potassium pump, pump sodium ions are exchanged for potassium ions. Every cycle of the pup moves three sodium ions out of the cell and brings two potassium ions in. Some Ion Pumps Generate Voltage Across Membranes Voltage is electrical potential energy. The cytoplasm of a cell is negative in charge compared to the extracellular fluid because of an unequal distribution of anions and cations on opposite sides of the membrane. The membrane potential, potential voltage across a membrane, ranges from about -50 to -200 millivolts. The membrane potential acts as an energy source that affects traffic of charges substances. Since the inside of the cell is negative compared to the outside, the membrane favors passage of cations inside the cell and anions outside the cell. Thus, ions are driven by a chemical force (concentration gradient) and electrical force. This combination is the electrochemical gradient. gradient An ion diffuses down its electrochemical gradient. With each cycle of the sodium-potassium pump, there is a net transfer of one positive charge from the cytoplasm to the extracellular fluid. A transport protein that stores energy in the form of voltage is an electrogenic pump. pump The main electrogenic pump of plants, bacteria, and fungi is a proton pump, pump which actively transports hydrogen ions (protons) out of the cell. This transfers positive charge from the cytoplasm to the extracellular solution. In Cotransport, a Membrane Protein Couples the Transport of Two Solutes A single ATP-powered pump that transports a specific solute can indirectly drive the active transport to several other solutes in a mechanism called cotransport. cotransport A substance that has been pumped across a membrane can do work as it leaks back by
6 UNIT TWO: THE CELL Chapter Eight: Membrane Structure and Function (Text from Biology, 6th Edition, by Campbell and Reece) diffusion. This is like pumping water downhill and then having it power a windmill as it flows downward. Another specialized transport protein can couple the downhill diffusion to the uphill transport against its own concentration gradient. For example, the gradient of hydrogen ions generated by proton pumps can be used to drive the active transport of amino acids, sugars, and several other nutrients into the cell. The protein can move sucrose into the cell only if the sucrose is accompanied by a hydrogen ion. Thus, the sucrose will follow the hydrogen ion into a transport protein. Exocytosis and Endocytosis Transport Large Molecules Larger molecules must travel through the cell membrane via vesicles. Exocytosis is the secretion of molecules by sending vesicles outwards to the plasma membrane. A transport vesicle can bud off the Golgi apparatus then merge with the plasma membrane. Exocytosis is used to export products out of a cell. In endocytosis, endocytosis the cell takes in macromolecules and particulate matter by forming vesicles from the plasma membrane. There are three types of endocytosis. In phagocytosis, phagocytosis a cell engulfs particle by wrapping pseudopodia around it and packaging it into a vacuole. This particle can then be digested when fused with a lysosome. In pinocytosis, pinocytosis droplets of extracellular fluid are put into tiny vesicles. ReceptorReceptor-mediated endocytosis is very specific. Proteins with specific receptor sites are exposed to the extracellular fluid. Ligands are any molecule that binds specifically to the receptor site of another molecule. The receptor proteins are usually clustered in regions called coated pits, which are lined by a layer of protein. When ligands bind to receptor proteins, a vesicle will break off and transport materials into the cell. Cells can acquire bulk quantities of specific substances through this form of endocytosis. For example, cholesterol is taken into the cell through this process.