TB16srrna hrm-10sample with H37Rv-20.03.

13
Experiment Creation Date 20-03-2013 13:50:24 Operator Dr Chitra Pai Start Time 20-03-2013 13:53:09 Run State Completed Macro Macro Status Templates Test ID Color Comp ID Run Notes Programs Program Name PreIncubation Cycles 1 Target (C) 95 Acquisition Mode None Analysis Mode None Hold (hh:mm:ss) 00:10:00 Ramp Rate (C/s) 4.40 Acquisitions (per C) Sec Target (C) 0 Step size (C) 0 Step Delay (cycles) 0 Plate ID 02554124 Lot ID Last Modified Date 20-03-2013 16:08:34 Owner operator End Time 20-03-2013 15:11:17 Software Version LCS480 1.5.0.39 Macro Owner

Program Name Amplification Cycles 45 Target (C) 95 65 72 Acquisition Mode None Single None Analysis Mode Quantification Hold (hh:mm:ss) 00:00:10 00:00:15 00:00:10 Ramp Rate (C/s) 4.40 2.20 4.40 Acquisitions (per C) Sec Target (C) 0 0 0 Step size (C) 0 0 0 Step Delay (cycles) 0 0 0

Program Name Melting Curve Cycles 1 Target (C) 95 40 65 95 Acquisition Mode None None None Continuous Analysis Mode Melting Curves Hold (hh:mm:ss) 00:01:00 00:01:00 00:00:01 Ramp Rate (C/s) 4.40 2.20 4.40 0.02 25 Acquisitions (per C) Sec Target (C) 0 0 0 0 Step size (C) 0 0 0 0 Step Delay (cycles) 0 0 0 0

Program Name Cooling Cycles 1 Analysis Mode None

TB16srrna hrm-10sample with H37Rv-20.03.13

20-03-2013

Page 1 of 5

Target (C) 40 Samples

Acquisition Mode None

Hold (hh:mm:ss) 00:00:10

Ramp Rate (C/s) 2.20

Acquisitions (per C)

Sec Target (C) 0

Step size (C) 0

Step Delay (cycles) 0

Sample Count 96 Subset All Samples Pos A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 Name Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12 Sample 13 sd h37rv 10-8-h37rv v b chaudhary rukmini sayyad shreedhar rasheeda 5-311 18-905 nitin Sample 24 Sample 25 16-738 10-8 10-8 rukmini only mm only water Sample 32 Sample 33 Sample 34 Sample 35 Sample 36 20-03-2013 Page 2 of 5 ID Repl. Of Sample Notes

TB16srrna hrm-10sample with H37Rv-20.03.13

Samples Sample Count 96 Subset All Samples Pos D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 Name Sample 37 Sample 38 Sample 39 Sample 40 Sample 41 Sample 42 Sample 43 Sample 44 Sample 45 Sample 46 Sample 47 Sample 48 Sample 49 Sample 50 Sample 51 Sample 52 Sample 53 Sample 54 Sample 55 Sample 56 Sample 57 Sample 58 Sample 59 Sample 60 Sample 61 Sample 62 Sample 63 Sample 64 Sample 65 Sample 66 Sample 67 Sample 68 Sample 69 Sample 70 Sample 71 Sample 72 Sample 73 Sample 74 Sample 75 20-03-2013 Page 3 of 5 ID Repl. Of Sample Notes

TB16srrna hrm-10sample with H37Rv-20.03.13

Samples Sample Count 96 Subset All Samples Pos G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 Name Sample 76 Sample 77 Sample 78 Sample 79 Sample 80 Sample 81 Sample 82 Sample 83 Sample 84 Sample 85 Sample 86 Sample 87 Sample 88 Sample 89 Sample 90 Sample 91 Sample 92 Sample 93 Sample 94 Sample 95 Sample 96 ID Repl. Of Sample Notes

Revision History

Revision Date

User

Reason

Abs Quant/2nd Derivative Max for New Subset 1 (Abs Quant/2nd Derivative Max)
Settings Channel 465-510 Color Compensation Off Program Amplification Mode High Confidence Subset Name New Subset 1 Results Inc Pos Name B2 sd h37rv Type Unknown CP 19.01 Concentration Standard Status Units

TB16srrna hrm-10sample with H37Rv-20.03.13

20-03-2013

Page 4 of 5

Results Inc Pos Name B3 B4 B5 B6 B7 B8 B9 10-8-h37rv v b chaudhary rukmini sayyad shreedhar rasheeda 5-311 Type Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown CP 17.01 27.31 25.06 24.66 24.29 25.54 25.46 25.25 26.45 25.96 17.56 17.17 24.57 24.07 Concentration Standard Status

B10 18-905 B11 nitin C2 C3 C4 C5 C6 C7 16-738 10-8 10-8 rukmini only mm only water

Tm Calling for New Subset 1 (Tm Calling)

TB16srrna hrm-10sample with H37Rv-20.03.13

20-03-2013

Page 5 of 5