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Biology I
Bellringer:
Rashawn wanted to see if he could improve the gas mileage in his Porsche. He decided the best way to
test this was to try different types of fuels. He completed 5 different trials that are outlined in the
following table:
Type of Fuel
Gasoline
Water
Kool-Aid
Vinegar
Cat Urine
Todays Objective: I can determine whether an unknown substance contains a living organism
through experimentation
2. You can buy yeast to make bread in the grocery store. This yeast consists of little brown grains. Do
you think that these little brown grains of yeast are alive? Why or why not?
3. To find out whether yeast is alive, we first need to think about what makes something alive. What
are some characteristics of living organisms?
Background Information:
To begin to answer the question, "Is yeast alive? you will test whether the grains of yeast have a
characteristics of living things -- the ability to use energy (referred to as metabolism).
We will carry out an indirect test for metabolism. In other words, we will be indirectly testing whether
yeast can use energy, which is one of the characteristics of living organisms.
When yeast, humans, and other living organisms use energy, they break down high-energy molecules
like sugar to get the energy they need and give off a gas called carbon dioxide as a by-product of this
reaction.
We will test whether yeast can metabolize sugar and produce a gas which we will presume is carbon
dioxide. Specifically, we will test whether yeast produces a gas when it has sugar available as a food
vs. when no sugar is available.
Pre-Lab Questions:
1. What is the research question or problem?____________________________________
________________________________________________________________________
2. What is the independent variable? __________________________________________
3. What is the dependent variable? ___________________________________________
4. What is your hypothesis? If _______________________________________________
then ____________________________________________________________________
Teachers Initials:________________
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Test tubes 3 and 4 will both have only yeast and water, with no sugar.
3. Fill test tube #1 full with warm tap water. Add one packet of dry yeast a little bit at a time,
mixing the yeast in thoroughly before adding more. Mix by using a popsicle stick and/or putting
your hand or thumb over the top of the test tube and shaking.
4. Pour the yeast solution so that there is an equal amount in each of the four test tubes.
5. Add packet of sugar to test tube 1 and the other half to test tube 2. These tubes will be your
balloon to seal the end of the tests tube, hold a finger over the end of each test tube and shake it
vigorously to thoroughly mix the contents.
8. Observe the test tubes and record your observations carefully in the table on the next page under
0 minutes.
9. Using a timer to keep time, every 5 minutes for 20 minutes, observe what occurs in the test tubes
and any changes in the balloons which cover each test tube, and record your observations.
10. While you are waiting to make observations, you should be finishing your lab from last class,
5 minutes
10 minutes
15 minutes
20 minutes
Test tube 1
(with
sugar)
Test tube 2
(with
sugar)
Test tube 3
(water)
Test tube 4
(water)
Biology I
Name: ________________________
Date: _________________________
Hookworms live in the human intestine drinking the blood it sucks from the intestine wall. It is
estimated that a single hookworm can drink .5mL of blood per day. The chart above contains
data on the number of hookworms and the amount of blood lost caused by that number of
worms.
A group of scientists decided to study the effect of hookworms on blood loss. They measured
how much blood was lost per day in patients with different numbers of hookworms in their
body. The data they collected is shown below:
Number of Hookworms (hookworms)
24
45
80
88
63
1. What type of symbiotic relationship exists between hookworks and humans (mutualism,
commensalism, parasitism, predation, or competition)? ___________________________
2. What is the independent variable? _________________________________
3. What is the dependent variable? ___________________________________
4. On the back of this page, create a graph of the data.
CONTINUE ON BACK TO CREATE GRAPH!
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