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COURSE OUTLINE
Kulliyyah Science
Department Biotechnology
Level 2
Credit Hours 3
Pre-requisites Nil
(if any)
Co-requisites Nil
(if any)
Course LO Method %
Assessment 1,2,4,5 Quizzes 15
State weightage 1,3, Laboratory reports 15
of each type of 1,2,4,5 Mid-term examination 25
assessment. 1,2,4,5 Final Examination 45
TOTAL 100
Instructor(s) Dr. Huzwah Khaza’ai
Telephone No.: 03- 6196 5449
email: huzwah@iiu.edu.my
Semester Semester I
Offered
1
Course Synopsis The history of tissue culture. Laboratory requirement, organization and
general techniques, animal cell culture; tissue culture media and
preparation. Plant cell culture; Zygotic embryo culture; callus and free cell
culture; evidence for totipotency; somatic embryogenesis; organogenesis;
anther and pollen culture. Isolation, culture and fusion of protoplasts.
Genetic stability and somaclonal variation. Experimental design and
quantification of results. Quantification and determination of cell viability,
kinetics and cell growth. Microbial contamination and prevention
procedures. Establishment and characterization of cell lines.
Learning By the end of the course the student will be able to: -
Outcomes 1. Describe the basis of the main procedures of cell line development,
cell engineering, cell banking, cell characterisation and media
formulation.
2. Discuss the physiological responses of animal cells to environmental
conditions in culture vessels, including those arising from nutrient
limitation and adverse hydrodynamic effects, and understand and
describe the principles of growth, death, metabolism and production.
3. Perform techniques pertinent to the growth and maintenance of cell
culture and able to perform experiments related to medical and
biotechnological processes.
4. Describe the methods and technologies for the (large-scale)
production of therapeutic proteins, gene therapy vectors and vaccines
in animal cells.
2
Content Outlines
3
12 Somatic Embryogenesis and Organogenesis Chapters
Background and discussions. 6&8
[2]
13 Micro-propagation, Anther Culture for Haploid Plant Chapters
Production 10 & 11
Background and discussions. [2]
Recommended
1. Harrison, M.A., & Rae, I.F., (1997), General Techniques of Cell
Culture, Cambridge University Press.
2. Dixon, R.A., (1985), Plant Cell Culture: Practical Approach, IRL
Press.
3. Doyle, A., & Griffiths, J.B., (2000), Cell and Tissue Culture for
Medical Research, John Wiley and Sons.
4. Chawla, H.S., (2002), Introduction to Plant Biotechnology, 2nd Ed.,
Science Publisher.
NOTE:
1. The course outlines should reflect the course description/synopsis and Islamic mission of the
university as well as to provide the room for Islamic critique.
4
2. Latest editions of textbooks and references should be used unless otherwise necessary or if
the course is classical in nature. Please check with the library to ensure that the books are
up-to-date and incorporating the latest edition.
3. The reference lists shall be presented in accordance with APA bibliographic practices and in
alphabetical order.
4. The reference title shall be italicised or underlined or bold. If in doubt, please consult the
Librarian.
5. Proposed new course outlines should be presented to the Senate Standing Committee for
approval at least four months prior to the course offering.
6. Any changes to the course outlines must be reported back to the Standing Senate Committee
two months prior to the course offering.