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INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA

COURSE OUTLINE

Kulliyyah Science

Department Biotechnology

Programme Bachelor of Biotechnology (BBT)

Course Title Principles of Cell and Tissue Culture

Course Code SBT 2131

Status Core Course

Level 2

Credit Hours 3

Contact Hours 5 (2 lecture hours and laboratory hours per week)

Pre-requisites Nil
(if any)

Co-requisites Nil
(if any)

Instructional The instructional strategies are:-


Strategies 1) Lecture
2) Lab practical
3) Quiz
4) Presentation

Course LO Method %
Assessment 1,2,4,5 Quizzes 15
State weightage 1,3, Laboratory reports 15
of each type of 1,2,4,5 Mid-term examination 25
assessment. 1,2,4,5 Final Examination 45
TOTAL 100
Instructor(s) Dr. Huzwah Khaza’ai
Telephone No.: 03- 6196 5449
email: huzwah@iiu.edu.my

Semester Semester I
Offered
1
Course Synopsis The history of tissue culture. Laboratory requirement, organization and
general techniques, animal cell culture; tissue culture media and
preparation. Plant cell culture; Zygotic embryo culture; callus and free cell
culture; evidence for totipotency; somatic embryogenesis; organogenesis;
anther and pollen culture. Isolation, culture and fusion of protoplasts.
Genetic stability and somaclonal variation. Experimental design and
quantification of results. Quantification and determination of cell viability,
kinetics and cell growth. Microbial contamination and prevention
procedures. Establishment and characterization of cell lines.

Course The objectives of the course are to: -


Objectives 1. Introduce the underlying principles of aseptic culture of animal, plant
or organs.
2. To provide information about equipment, procedures and terminology
of aseptic culture.
3. Provide an understanding of the advantages of using cell culture
system and illustrate the differences between techniques employed in
plant and animal cell culture.
4. Provide students with an understanding of the benefits and issues of
tissue culture to the ummah.

Learning By the end of the course the student will be able to: -
Outcomes 1. Describe the basis of the main procedures of cell line development,
cell engineering, cell banking, cell characterisation and media
formulation.
2. Discuss the physiological responses of animal cells to environmental
conditions in culture vessels, including those arising from nutrient
limitation and adverse hydrodynamic effects, and understand and
describe the principles of growth, death, metabolism and production.
3. Perform techniques pertinent to the growth and maintenance of cell
culture and able to perform experiments related to medical and
biotechnological processes.
4. Describe the methods and technologies for the (large-scale)
production of therapeutic proteins, gene therapy vectors and vaccines
in animal cells.

5. Illustrate knowledge of the principles of plant genetic engineering


technologies, and how they are applied to the production of transgenic
crops.

2
Content Outlines

Weeks Topics Task/Reading


1 Animal Cell Culture Chapters
An overview of background, advantages, limitations, terms 1 & 12
and definitions, growth of attachment-dependent and [1]
suspended cells; cell lines.

2 Biology of Cultured Cell Chapters


Culture environment, cell adhesion, cell proliferation, 3&4
differentiation and evolution of cell line. [1]

3 Design, Layout and Equipment in Animal Cell Culture Chapters


Construction, layout; essential, beneficial and useful 3&4
equipment; consumable items. [1]

4 Aseptic Technique and Safety Chapters


Objectives, element of aseptic environment, sterile handling, 5&6
general safety, risk assessment, radiation and biohazard. [1]

5 Media and Serum-free Media Chapters


Physicochemical properties, complete media, serum, 8 & 9
selection, advantages and disadvantages of serum free media, [1]
replacement of serum, media development.

6 Primary Culture and Cell Lines Chapter


Type of primary culture cell. Isolation of tissues. Primary 11
culture. [1]

7 Cloning Selection and Molecular Techniques Chapters


An overview of suspension cloning, isolation of clones, in 13 & 27
situ molecular hybridization, production of monoclonal [1]
antibodies. Somatic cell fusion and DNA transfer.

8 Cryopreservation and Quantification Chapters


Preservation, cell banks, transporting cells. Quantification. 19 & 20
[1]
9 Introductory to plant cell culture Chapters
Overview of plant cell culture. 2&3
[2]
10 The principles of aseptic culture in plants Chapter 4
Basic media components for plant cell culture. [2]

11 Organ, Callus Culture and Cell Suspension Chapters


Culture 5, 7 & 9
Background and discussions. [2]

3
12 Somatic Embryogenesis and Organogenesis Chapters
Background and discussions. 6&8
[2]
13 Micro-propagation, Anther Culture for Haploid Plant Chapters
Production 10 & 11
Background and discussions. [2]

14 Protoplast Isolation, Culture and Plant Regeneration Chapters


Applications of plant tissue culture. 13 & 14
[2]
References Required
1. Freshney, R.I., (2000), Culture of Animal cell: A Manual of Basic
Techniques, 4th Ed., Wiley Liss.
2. Dodds, J.H., & Roberts, L.W., (1995), Experiment in Plant Tissue
Culture, 3rd Ed., Cambridge University Press.

Recommended
1. Harrison, M.A., & Rae, I.F., (1997), General Techniques of Cell
Culture, Cambridge University Press.
2. Dixon, R.A., (1985), Plant Cell Culture: Practical Approach, IRL
Press.
3. Doyle, A., & Griffiths, J.B., (2000), Cell and Tissue Culture for
Medical Research, John Wiley and Sons.
4. Chawla, H.S., (2002), Introduction to Plant Biotechnology, 2nd Ed.,
Science Publisher.

Proposed Semester I, 2003/2004


Start Date
(Semester)
Batch of Batch 2002
Students to
be Affected

Prepared by: Checked by: Approved by:

__________________ _____________________ _____________________


Dr. Huzwah Khaza’ai Noor Faizul Hadry Nordin Prof. Dr. Ridzwan Hashim
Assistant Professor Head, Department of Dean, Kulliyyah of Science
Biotechnology

NOTE:
1. The course outlines should reflect the course description/synopsis and Islamic mission of the
university as well as to provide the room for Islamic critique.

4
2. Latest editions of textbooks and references should be used unless otherwise necessary or if
the course is classical in nature. Please check with the library to ensure that the books are
up-to-date and incorporating the latest edition.
3. The reference lists shall be presented in accordance with APA bibliographic practices and in
alphabetical order.
4. The reference title shall be italicised or underlined or bold. If in doubt, please consult the
Librarian.
5. Proposed new course outlines should be presented to the Senate Standing Committee for
approval at least four months prior to the course offering.
6. Any changes to the course outlines must be reported back to the Standing Senate Committee
two months prior to the course offering.

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