Group 1

Agustin, Vanessa Jaafar, Abdul Fadz Jauhali, Sharhana Ramos, Cheery Greek Rodriguez, Chrislouie Sarreal, Dolrich

1. . coli !as incubated !ith aeration in a nutrient medium containing t!o carbon sources, and the follo!ing gro!th cur"e !as made from this culture.

log1% of no. of cells

$ime

a.

&plain !hat happened at the time &.

At the time &, the . coli has depleted the glucose carbon source, therefore shifting there meal to their least preferred food, the lactose . . coli prefer to consume glucose and only use lactose after the glucose has been depleted.

b. 'hich substrate pro"ided better gro!th conditions for the bacteria( )o! can you tell(
$he glucose pro"ided the better gro!th conditions for the bacteria than the lactose. *n the graph, during the consumption of glucose by the . coli there is a great increase in the number of cells. After the glucose is depleted, the . coli utilizes the lactose as a carbon source there is a decrease in the number of cells than that of !hen they consumed the glucose because glucose is a

+. Clostridium and Streptococcus are both catalase-negati"e. Streptococcus gro!s by fermentation. 'hy is clostridium killed by o&ygen, !hereas streptococcus is not.
Clostridium is an obligate anaerobes !hich means they are unable to utilize o&ygen for energy yielding reactions. $hey lack enzymes to neutralize harmful forms of o&ygen that is !hy they cease to e&ist in the presence of o&ygen, !hile streptococcus is an aerotolerant anaerobes that gro!s by means of fermentation(anaerobic gro!th). ,&ygen has no effect because of the presence of an enzyme called supero&ide dismutase(S,D) that allo!s harmful forms of o&ygen to be partially neutralize.

-uchisimas Gracias