Exercise 2: Skeletal Muscle Physiology: Activity 2: The Effect of Stimulus Voltage on Skeletal Muscle Contraction Lab Report Pre-lab

Quiz Results You have not completed the Pre-lab Quiz.

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Experiment Results Predict Question: Predict Question: As the stimulus voltage is increased from 1.0 volt up to 10 volts, what will happen to the amount of active force generated with each stimulus? Your answer : c. The active force will first increase and then plateau at some maximal value as the stimulus voltage increases. Stop & Think Questions: What do you see in the active force display when the stimulus voltage is set to 0.0, and why does this observation make sense? You correctly answered: a. 0.00 g; there was no activation of skeletal muscle fibers by this stimulus. What is the lowest stimulus voltage that induces active force in the skeletal muscle? You correctly answered: b. threshold voltage 6. Enter the threshold voltage for this experiment in the field below and then click Submit to record your answer in the lab report You answered: 0,8 volts 12. Enter the maximal voltage for this experiment in the field below and then click Submit to record your answer in the lab report. You answered: 10,0 volts Experiment Data: Voltage 0.0 0.2 0.8 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 Length 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 Active Force 0.00 0.00 0.02 0.15 0.43 0.66 0.87 1.04 1.19 1.32 1.42 1.51 1.59 1.65 1.70 1.74 1.78 1.81 1.82 1.82 1.82 1.82 Passive Force 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total Force 0.00 0.00 0.02 0.15 0.43 0.66 0.87 1.04 1.19 1.32 1.42 1.51 1.59 1.65 1.70 1.74 1.78 1.81 1.82 1.82 1.82 1.82

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Post-lab Quiz Results You scored 40% by answering 2 out of 5 questions correctly. 1. Motor unit recruitment refers to You correctly answered: a. an increase in the number of active muscle fibers to increase the force developed in a muscle. 2. Active tension (or force) in a skeletal muscle fiber results from Your answer: d. detachment of the tendons from the bones in the body. Correct answer: a. activation of cross bridge cycling via increased intracellular calcium levels. 3. The ________ is the minimal stimulus needed to cause a depolarization of the muscle plasma membrane (sarcolemma). You correctly answered: d. threshold voltage 4. By definition, the ________ is the amount of stimulus required to successfully recruit all the muscle fibers into developing active force. Your answer: b. contractile stimulus voltage Correct answer: c. maximal voltage 5. Why was a maximal voltage observed in this experiment? Your answer: a. The maximal voltage induces the most calcium release from a muscle fiber's sarcoplasmic reticulum. Correct answer: b. At the maximal voltage, all the muscle fibers contained in this muscle are depolarized and they all develop active force (that is, they were all successfully recruited).

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Review Sheet Results 1. Describe the effect of increasing stimulus voltage on isolated skeletal muscle. Specifically, what happened to the muscle force generated with stronger electrical stimulations and why did this change occur? How well did the results compare with your prediction? You did not answer this question. 2. How is this change in whole-muscle force achieved in vivo? You did not answer this question. 3. What happened in the isolated skeletal muscle when the maximal voltage was applied? You did not answer this question.

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