Table 2.

Parameters of gradient elution Time (min) 0 30 35 55 56 60 % A (V/V) 100 15 15 0 0 100 % B (V/V) 0 85 85 100 100 0

V/V) and mobile phase B mixture of methanol/water (95:5, V/V) were used after addition of acetic acid (to 1%, pure, min. 85%, Lachema Neratovice, Czech Republic). Elution time 42 min, injection of sample 20 L, detection at = 350 nm. Quantitative estimation of the concentration of individual compounds was performed by the method of absolute calibration. Quercetin (> 98%, HPLC, Fluka AG) and rutin (> 99%, HPLC, Fluka AG) were used as standards. RESULTS AND DISCUSSION Average total polyphenol content (TP) in cv. Ala (white colour) was 26,445 mg/kg DM, in cv. Vetana (yellow) 65,210 mg/kg DM and in cv. Karmen (red)

20 L, detection at = 250 nm (parameters of gradient elution are given in Table 2). For the determination of quercetin and its glycosides mobile phase A mixture of methanol/water (5:95,

Fig 2. TP-time and TP-temperature storage graphs in cv. Ala. REF stored in refrigerator (4C); LAB stored at laboratory temperature (22C)
100,000 90,000 Total polyphenols (mg/kg DM) 80,000 70,000 60,000 50,000 40,000 30,000 20,000 0 5 10 15 20 25 30 35 40 Weeks of storage
R 2 = 0.0365
2 R == 0.5425 R2 0.5425

yellow REF yellow REF yellow LAB yellow LAB linear (yellow REF) linear (yellow REF) linear (yellow LAB) linear (yellow LAB)

Fig 3. TP-time and TP-temperature storage graphs in cv. Vetana HORT. SCI. (PRAGUE), 30, 2003 (4): 142147

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