Beruflich Dokumente
Kultur Dokumente
Dr Iram Iqbal
Aim
To define and discuss different type of
mounting medias ,their technique for
mounting sections ,and the qualities
of the ideal mountant.
sequence
Defination
Qualities of an ideal mountant
Classification of mounting media
Cover glasses
Properties of the good mounted sections
Technique for mounting sections
Labelling & and storage of slides
MOUNTING OF
SECTIONS
After staining and before
examination, sections should be
mounted with a suitable medium under
cover slips .
To facilitate ease of handling and
storage and to prevent damage to
the section.
The liquid used for mounting slides is
known as “Mountant”.
Qualities of an ideal
mountant
An ideal mounting medium must fulfill
following criteria;
It should fulfill the basic
transparent .
AN IDEAL MOUNTANT
The medium should have
a refractive index as
close as possible to that
of the glassi,e,; 1.5 .
It should not cause
stains to “bleed”or fade
It should not crack or
appear granular on
setting.
It should be non sticky
and harden quickly.
Cont…
It should not shrink back from the
edges of the cover glass.
It must be free flowing.
It must be free from bubbles.
It should be resistant to
contamination
Once set, the mountant should remain
stable.
Types of mounting Media