See.

: Name: _
Experiment: Characteristics of Prokaryotic and
Eukaryotic Cells (8. Science 10-7-3a)

Purpose: To examine the differences between

prokaryotic and eukaryotic cells. Then to use
these differences to classify an unknown
specimen.

Materials: microscope lens paper

prepared microscope slides (human or frog blood,
epithelial cells, x.s. of leaf, amebas, and bacteria
or cyanobacteria (Anabaena), unlabeled cell for
unknown

Safety Caution: Care with microscopes,

glassware caution.
MetnOQs:
1. Obtain a microscope and place 10 cm from edge of table.
2. Carefully clean the eyepiece and objective lens with lens paper.
3. Place your first prepared slide on the microscope stage so that it is centered over
the stage opening. Hold the slide in position with the stage clips.
4. Using the low- power objective lens, locate the cell(s) under the microscope. Turn
the coarse adjustment knob until the cell comes into focus.
5. In the Data table, the type of cell that you examined is already recorded.
D9scribe the general shape of the cell in the space provided. Put a check marK below
the cell structures you are able to observe under the low power.
6. SWitch to the high - power objective lens. CAUTION: When turning to the high-
power objective lens, you should always look at the objective from the side of your
microscope so that the objective lens does not hit or damage the slide. Look for ceH
structures unobservable under low power. Put a check mark below these structures in
the table. Based on your observations, decide if the cell is prokaryotic or eukaryotic
and record this in the data table by writing "E" for eukaryotic or "P" for prokaryotic.
7. In the appropriate place in observations, draw and label what you see using the
high - power objective lens. Record the magnification of the microscope.
S. Repeat steps 1 through 7 using other prepared slides.
9. Repeat steps 1 through 7 using an unidentified prepared slide.

Results: cell cell nuclear prokaryoticl

Cell type shape wall membrane nucleus envelope cytoplasm vacuoles eukary.
blood ___ I I I I I I _, _
elOithelial _, _
leaf

amebas

bacteria

unknown
 See.: Name: .

x x x

blood epithelial cells cross section of leaf

x x x
amebas bacteria unknown

Conc!usions:
1. Based on your observations, do all cells have the same shape? Support your
answer.

2. Based on your observations, do all cells have the same size? Support your
answer.

3. What cell structures are common to all cells?

4. What cell structures are found only in eukaryotic cells?

5. Are the nuclei always found in the same place within different types of cells?
Support your answer.

Discussion:
1. Skin cells seem to fit together like pieces of a jigsaw puzzle. How is the
arrangement of cells helpful to an organism?

2. Why do cells have different shapes and sizes?

3. What cell structure might you be able to compare to the main (principal's) office in
your school? Explain your answer.

4. Scientists have grouped all cells into two categories, eukaryote & prokaryote. How
does God categorize human cells (flesh) and other cells (I Cor. 15:39)?