PCR 1. Extract DNA, and incubate for about 20 minutes 2.

Make a mixture using the following recipe

The number of samples you 14.8 L have +1 for wild type. This 2.4 L number than round it to a 0.96 L whole number (such as 10, 20, 0.24 L ect). Multiply this number to 1.2 L the ingredient in each row; 1.2 L this is the amount you use for 1.2 L the large mixture. 1 L Sum 23 L (mix well using a large pipette) Take 23 L of the large mixture and add to each tube. Then add 1 L of sample to each tube. Centrifuge each sample for 1 minute. 3. PCR setting on machine. Preheat.

H2O 10x PCR MgCl2 dNTP Primer A Primer B Primer C Taq

Gel Electrophoresis 1. Making the gel a. Set the clear tray in gel electrophoresis machine so that the tray is locked in place. Place the appropriate comb in the machine b. Weight agarose powder. PS: The calculation: 1.5g agarose / 100mL of 1xTAE (1.5% agarose concentration). The concentration varies by the length of DNA, shorter length=higher concentration. c. Add clean 1x TAE solution (large container) to the flask containing agarose powder d. Heat the solution in a microwave until it boils (the solution at this time should be completely clear, with no small bubbles) e. Add 5L of EB to the hot solution and swirl the solution in flask until completely mixed f. Let cool for about 5 minutes, then pour into the tray g. Allow the gel to set 2. Remove the gel and cut it in half. Place the gel correctly in the tray 3. Running the gel a. 2 L of loading buffer + 10 L of sample b. 10 L of standard