STRAIN IMPROVEMENT Submitted by: JOSE, LANDICHO, MARTIN

The Effects of Ultraviolet Radiation on the fungal spores of Aspergillus niger

METHODOLOGY:

Microogranism

Aspergillus niger wild typer strain will be obtained from the Department of Science and Technology (DOST).

Inoculum preparation

50 ml of potato dextrose will be inoculated with two (2) loopfulls of Aspergillus niger slant that will be kept under aseptic conditions and will be incubated for three (3) days and used as a source of microorganisms for the UV radiation experiment.

UV mutagenesis

Spore suspension will be prepared in a petri plates placed under UV lamp (20w) at a 10cm distance of 0 to 120 minutes with regular interval of 30 minutes. After irradiation of spores, were cultured on PDA plates incubated at room temperature for three (3) to five (5) days until sporulation of fungal culture.

STRAIN IMPROVEMENT Submitted by: JOSE, LANDICHO, MARTIN FLOW DIAGRAM:

Inoculum Prepration Inoculation of Aspergillus niger

Transfer of A. niger suspension

Exposure of A. niger under UV light Morphological Observation

On sterile petri dishes

Time interval of 0- 120 minutes with regular interval of 30 minutes.

Cell viability staining by Methylene Blue

ILLUSTRATIONS: Figure A. UV light interval Unexposed culture medium

0 min

30 mins.

60 mins.

90 mins.

120 mins.

STRAIN IMPROVEMENT Submitted by: JOSE, LANDICHO, MARTIN Figure B. Serial Dillution 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml

= 2.5 ml 101 102 103 104 105 106

4.5 ml

0.5 ml -> 0 minutes -> serial dilution 0.5 ml -> 30 minutes -> serial dilution 0.5 ml -> 60 minutes -> serial dilution 0.5 ml -> 90 minutes -> serial dilution 0.5 ml -> 120 minutes -> serial dilution Figure C. Survival Chart

1.2 1 0.8 CFU 0.6 0.4 0.2 0 0 30 60 TIME (minutes) 90 120