2702

(J South Med Univ)

2010;30(12)

NR2B
/ 510080
(CCI) NR2B
SD 200~280 g 40 4 CCI
CCI CCI CCI 713 d Western
blot NR2B CCI CCI CCI
P<0.05 CCI P<0.05 CCI NR2B P<0.05
NR2B P>0.05
CCI NR2B
NMDA
R616.1

1673-4254(2010)12-2702-03

Effect of electroacupuncture on pain threshold and spinal NR2B subunit expression in a rat
model of neuropathic pain
ZHOU Guo-bin, MA Wei-tao, YU Wei, GUO Ai-lin, JI Jin-quan
Department of Anesthesiology, Guangdong General Hospital, Guangzhou 510080, China
Abstract: Objective To observe the effect of electroacupuncture (EA) on pain threshold and spinal NR2B subunit expression
in a rat model of neuropathic pain due to chronic compression injury (CCI) of the sciatic nerve and explore the analgesic
mechanism of EA. Methods Male SD rats weighing 200-280 g were randomly divided into 4 groups (n=10), namely the
sham-operated group, CCI group, EA+CCI group, and sham EA+CCI group. All the rats underwent tests of the mechanical
withdrawal threshold and thermal threshold. On day 13 after the surgery, all the rats were decapitated to collect the L4-6
segments of the spinal cord to examine NR2B expression using Western blotting. Results The postoperative mechanical
withdrawal threshold and thermal threshold were significantly lowered in CCI, EA+CCI and sham EA+CCI groups as
compared to those before the surgery (P<0.05). EA obviously alleviated the hypersensitivity in the rats with CCI and inhibited
spinal NR2B expressions (P<0.05). No significant differences were found in the mechanical withdrawal threshold, thermal
threshold or spinal NR2B subunit expression between CCI group and sham EA+CCI group (P>0.05). Conclusion EA may
alleviate neuropathic hypersensitivity partially by inhibiting NR2B expression in the spinal cord of rats with neuropathic pain
resulting from CCI of the sciatic nerve.
Key words: neuropathic pain; electroacupuncture; spinal cord; NMDA receptor

1-2N- -D

(NMDA)
3-5
NR2B

6-8
NR2B

2010-06-03
93048
(1966-)E-mail: tiramisu.yy@163.
com

1
1.1
SD 200~280 g
4
(CCI)(n=10)
CCI CCI n=10
CCI (n=10) 7
CCI (n=10) 7

1.2 CCI
1% 40 mg/kg

4-0 4

 12

2703

, . NR2B

1.3
7 d

CCI
P<0.05 13 dCCI

(2~100 Hz) 0.512 mA

7 d
7 d

10 min 1 30 min 1 4

7 d

1.4 CCI

P>0.05, 12

713
von Frey
8:00 am~5:00 pm
20~24

PWL 5 min 1 , 3

20 s
Von Frey
34

90
10 5

1 g, n=10

7 d

13 d

15.00.9

14.10.8

14.70.7

CCI

14.80.7

3.90.8*

5.30.9*

CCI

15.30.7

3.51.0*

10.21.1*#

CCI

14.70.5

3.31.1*

7.50.9*

*P<0.05 vs sham group #P<0.05 vs CCI group

2 s, n=10

7 d

13 d

16.12.3

15.12.8

16.73.7

CCI

15.82.1

5.91.8*

6.31.9*

CCI

16.03.0

6.52.0*

13.23.1#

14.92.8

5.32.1*

9.02.9*

CCI

*P<0.05 vs sham group #P<0.05 vs CCI group

13 d CCI

1.5 NR2B
13 d

CCI CCI NR2B

P<0.05 CCI CCI

L4-5
-80 Western blot

NR2B P<0.05
CCI NR2B

NR2B

50 g 7.5%SDS-PAGE
5% 1 h

4 NR2B (1500) TBS

(15000) 2 h ECL
NR2B -actin
NR2B
1.6

CCI NR2B
CCI
P>0.05, 3
3 13 d NR2B n=10

NR2B

25.373.89

CCI

78.656.27*

CCI

39.215.99*#

CCI

61.856.11*

*P<0.05 vs P<0.05 vs CCI

#

t
SPSS13.0 P<0.05

P>0.05 CCI

CCI CCI 7 d

2704

(J South Med Univ)

N- -D
(NMDA) NR1

30

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