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LABORATORY ACTIVITY REPORT FUNDAMENTAL OF BASIC SCIENCE II CHAPTER 2: ENZYME ACTIVITY

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Indah Permata Hasnul Nadiya Afifah Kemas Yasir Ratu Rediani Utari Ahda Febianti Aprilia Puspasari Yessi Cindy Agustin Dinda Dwi Gusdinar Fitria Nurhayati

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10. Putri Maratu Sholiha

UNIVERSITAS ISLAM BANDUNG FAKULTAS KEDOKTERAN 2013

DETERMINING FACTORS WHICH INFLUENCE AMYLASE ACTIVITY


I. AIM To find out the factors that influence the activity of amylase enzyme. II. PRINCIPLE Saliva is produced by the submaxillary, sublingual, and parotid glands, as well as by the mucouse membrane and buccal glands of the mouth, throat, and esophagus. Saliva contains about 99,5% of water. It also contains ptyalin or saliva amylase, several proteins of which mucin, a glycoprotein, is the most important, and a number of substances found in blood and urine such as ammonia, amino acids, urea, uric acid, cholesterol calcium, sodium, pottassium, magnesium, phospate, chloride, and bicarbonate. Ptyalin or saliva amylase acts on strach, producing a series of ill-defined products: amylodextrin, erythrodextrin, achrodextrin and maltose. The activity of salivary amylase is enchanced by the chloride ion. Salivary amylase acts as the 1,4-glucosidic linkages of amylase and amylopectin. The iodine serves as an indicator. A dark blue color indicates the presence of starch, the yellow iodine color means no starch remains, having been hydrolized by the amylase. III. TOOLS AND MATERIALS - Saliva (ptyalin enzyme) - Sodium chloride 0,1% - Sodium chloride 1% - Sodium chloride 5% - Aquadest - Starch: Amylum (substract) - Iodine 0,1 M (indicator) - small beaker - test tubes (6-10) - micropippete - stopwatch

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PROCEDURES

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RESULTS

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CONCLUSION 1) INFLUENCE OF COFACTOR (NaCl) 2) INFLUENCE OF TEMPERATURE 3) INFLUENCE OF pH

MEASURING ASPARTAT AMINO TRANSFERASE (AST) ACTIVITY


I. AIM To measure the activity level of AST enzyme. II. PRINCIPLE Aspartate Amino Trnsferase catalyses the transfer of Nitrogen from glutamate to oxaloacetate according to the following equation: For the quantitive AST determination the serum being tested is reacted in a buffered III. MATERIALS

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PROCEDURES

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CALCULATION AST activity (IU/L) : (A/minute) X factor Factor: 340 nm 1746

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RESULTS CONCLUSION