a9)
6
03)
3)
ay
@
(6)
2) Patent Application Publication
United States
‘Yang et al.
INFLUENZA HEMAGGLUTININ AND.
NEURAMINIDASE VARIANTS
Inventors: Chin-Fen Yang, San Jose, CA (US)
George Kemble, Saratoga, CA (US);
Kanta Subbarao, Washington, DC
(US); Brlan Murphy. Bethesda, MD
ws)
Comespondence Address
JOHNATHAN KLEIN-E)
‘ONE. MEDIMMUNE WAY
GAITHERSBURG, MD 20878 (US)
ANS
Assignees: Medimmune Vaeeines, Ines, Gaiters:
burg, MD (US); The Government of the
United States of Ameriea National
Institutes of Health, Rockville, MD (US)
Appl. Nos 11836,413
Bile: Aug. 9, 2007
Related U.S. Application Data
Provisional application No. 6(821:832, filed on Avg.
9, 2006, Provisional application No. 60042,804, fled
oon Jun. 8, 2007,
'US 2008006982141
ao) Pub, No.: US 2008/0069821 Al
(43) Pub. Date: Mar. 20, 2008
Publication Classification
(1) Ine
AGIK 39/145 (200601)
AIK 357% (2006.01)
61K 39/00 (2006.01)
AGIP 31/16 (2006.01)
chan 119 (2006.01),
CRN 1563 (2006.01)
CRN 71 (2006.01),
CRP 2100 (200601)
CRN Sd (200601)
CRN 121 (2006.01)
COTK 1402006001)
AGIK 39395 (2006.01)
A6IK 39/12 (208601)
I 424113941; 424/186.1; 424/206.1
4424/93,2; 435/235,1; 435/252.33
435/254.2; 435/325; 435/340:
435/350; 435/352; 435/364;
435/365; 435/69.3; 530/350
on ABSTRACT
Polypeptides, polynucleotides, methods, compositions, and
vaccines comprising (avian pandemic) infliazs hemagal-
‘inin and neuraminidase variants are provided.Patent Application Publication Mar. 20,2008 Sheet 1 of 31 US 2008/0069821 AI
GGA TTA TTT
Pro Gln Arg Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe
CCT CAA AGA GAG ACT CGA GGA TTA TIT
Pro Gln Arg Glu Thr Arg Gly Leu Phe
site of cleavage of into HA1 and HA2 domains.
jues that were mutagenized are underlined.
Fig 1.Patent Application Publication Mar. 20,2008 Sheet 2 of 31 US 2008/0069821 AI
Virus isolation from swabs
a
Mortality Oro ! Cloacal
Virus: (dead/total) pharyngeal detected/
‘etal
* # Mean logo
shedding/ ,MECTUORC, shedding/ titer
total 0) total (EID)
1997,
2008 ns
a a are M5 0 ou |
PONT wt
1987,
2008 cnt
aor ore oe 09 ore 0.9 oe
HEN ca
Chickens were inoculated intranasally with 108 TCIDyq of virus,
Fig 2.