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NILAI UNIVERSITY COLLEGE

DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY


MPAD 1221
PRACTICAL 2
Name :
Lim Jiang Jun
Date :
19.02.2009
Objectie
To determine Rhesus phenotype
I!t"#$%cti#!
Besides the Rh D antigen, there are also other antigens of the Rh system are C Rh2!, "
Rh#!, $ Rh%!, e Rh&!. 'ntigen(positi)e red $ells may stimulate anti*ody produ$tion in
C, $, " and e negati)e indi)iduals, and on$e it +as formed, the anti*odies may $ause
destru$tion of $orresponding red $ells. The determination of the Rh phenotypes is,
therefore, important during pregnan$y +ith ,no+n irregular anti*odies.
Rea&e!t'
DiaClon 'nti(CD", (C, ($, (", (e
0.9- isotoni$ saline solution
Sam()e
.hole *lood
C#!t"#)'
/no+n positi)e and negati)e samples should *e in$luded in a$$ordan$e +ith the rele)ant
guidelines of 0uality assuran$e.
P"#ce$%"e
1lide2Tile Test
1. 1 drop &03L! of the respe$ti)e reagent is pipette onto the slide2tile.
2. 1 drop &03L! of +hole *lood is added.
#. The mi4ture is mi4ed +ell +ith a $lean mi4ing sti$,.
%. 'gglutination is o*ser)ed ma$ros$opi$ally +hile rotating the slide2tile.
'gglutination o$$urs in most $ases )ery rapidly. 5nterpret the rea$tions +ithin 2 minutes.
Di'c%''i#!
A!ti*
CDE
A!ti*
C
A!ti*
c
A!ti*
E
A!ti*
e
A!ti*
D
P+e!#t,(
e
P#''ib)e Ge!#t,(e
1
.
6 6 6 0 6 6 DC$e CDe2$De , CDe2$de
R
1
R7! R
1
r!
2
.
6 6 0 0 6 6 DCe CDe2CDe , CDe2Cde
R
1
R
1
! R
1
r
y
!
#
.
6 6 6 6 6 6 DC$"e CD"2$De , CD"2$de
R
8
R
o
! R
8
r
y
!
$D"2CDe , $D"2Cde
R
2
R
1
! R
2
r9!
CDe2$d" , $De2Cd"
R
1
r:! R
o
r
y
!
%
.
6 6 0 0 6 6 DCe CDe2CDe , CDe2Cde
R
1
R
1
! R
1
r
y
!
The result sho+s all are rhesus positi)e patients. 'ntigen D is present in their *lood.
There are agglutination o$$ur on the tile means the antigen is present or )i$e )ersa.
Through this method, +e $an determine a patient;s phenotype either is positi)e or
negati)e and possi*le genotype $an *e found out.
C#!c)%'i#!
Rhesus antigen test is also one of the easiest test to +hi$h $an easily $ondu$t in the la*.
The test does not re0uire many reagents and +as one of the lo+ $osting tests. the test is
simple and easy a)aila*le.