Migration Plate Map

Experiment Details:
Cell Type:
Media:
Seeding Density (cells/well):
Chemoattractant:
Coating:
Basic Procedure (for details see separate document):
1. Trypsinize, pellet, resspend in serm !ree media (S"M)
#. To $ottom cham$er add 1%&'l o! media (/) 1&* "+S
,. -ssem$le top cham$er to $ottom cham$er and add .& ) 1&&'l cells to top cham$er.
/. 0nc$ate ,& mintes at room temperatre ) ndistr$ed
.. Cell 0n1asion monitored e1ery 1.min !or #/ hrs.
1 2
A Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
B Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
C Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
D Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
E Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
F Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
! Cell:
2m$er:
Coating: 2one
Chemoattractant:
Cell:
2m$er:
Coating: 2one
Chemoattractant:
3age 1 o! 1