Sanger Method

Use of Sequencing: ID new genes and associations w/ diseases and phenotypes

***Sanger Method
-Need DNA in single stranded form (Template DNA)
-Need DNA polymerase (to initiate DNA synthesis)
-dNTPs
-Single Primer

1. Add mixture of the above materials into four tubes each with a unique ddNTPs
(dideoxyribonucleotide) thats fluorescently stained
2. Replication continues w/ dNTPs base pairing
3. Synthesis of DNA terminated when ddNTPs pairs with template DNA
Result in each tube: multiple DNA strands

4. Run gel