BY

S.MATHAN KUMAR
VMKVEC, SALEM

A = b c

A absorbance (-)
molar absorbtivity with units of L mol-1
cm-1
b path length of the sample (cuvette)
c Concentration of the compound in
solution, expressed in mol L-1

Transmittance, T = I / I0,
Absorbance,

%T = 100 T
A= log10 I0 / I
A= log10 1/T
A = 2 - log10 %T

Amount of light that a sample absorbs.
Beam of monochromatic light
Measuring the intensity of light reaching a
detector

EFFECT S OF CONCENTRATION TRANSMISSION OF LIGHT SOURCE
ABS OF LIGHT
Deviations in absorptivity coefficients at
high concentrations(> 0.01M) due to
electrostatic interactions.
Scattering of light due to particulates in
the sample
Fluoresecence or phosphorescence of the
sample
Changes in refractive index at high analyte
concentration
Non-monochromatic radiation



BIO CHEMISRTY ANALYS
CELL COUNTING(FLUROSCENCE COUNTING)
Electrolyte
Like
ISOTON III
Oscilloscope
Sensing Zone
Neutrophil
Cell Counting principle
Oscilloscope
Cross section of
RBC aperture
(60x50 micron)
Oscilloscope screen
RBCs
PLT
+ -
Lymp
Mono
Eos
Neut
Baso
ADC
Signal
Processor
Lamp
CHAMBER
Sample
Sensor &
PRE-AMP
Absorbance= log
10
( VR / Vs )

Where VR = Reference voltage
Vs = Sample voltage
Derivation