Inulin and Erythritol As Sucrose Replacers in Short-dough Cookies: Sensory, Fracture, and Acoustic Properties
Original research conducted by Laura Laguna, Cristina Primo-Martin, Ana Salvador, and Teresa Sanz and provided in the Journal of Food Science November 2013
Bonnie Green NFS 2260: Introduction to Food Science April 13, 2014
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Table sugar (also known as sucrose), about 70% of which is acquired from sugar cane worldwide, has been used since about 1000 BC for everything from medicine to cooking (4). Nowadays, contrarily, sucrose causes people all sorts of health issues. Having 4 kilocalories per gram, sucrose can cause problems with people diagnosed with obesity, Type II diabetes, or dental cavities (1). People struggling with their weight often look for the sweet treats that they crave at the supermarket, but a lower-calorie version with less guilt. Diabetics need to be careful not to eat something concentrated in carbohydrates that will cause their blood sugar to spike out of control (5). Therefore, it is important to have a snack on the market that is lower in sucrose and calories, but is still flavorful and retains other pleasing cookie attributes, such as color, smell, surface features, texture, and hardness. Two compounds used in this study to replace sucrose in short-dough cookies will be inulin and erythritol. Inulin is a carbohydrate extracted from chicory or Jerusalem artichoke, and accomplishes the requirements for being considered dietary fiber. Humans can neither digest nor absorb inulin, so it contains no calories, it is fairly water soluble, and it can form a small, spreadable gel network (3). To date, there have been no studies using inulin as a sucrose replacer in cookies (1). Erythritol is a 4-carbon sugar alcohol with a mild sweetness. It looks similar to sucrose, and has been used to replace sucrose in baked goods, such as chiffon cake or Danish cookies. It increases cookie lightness and gets satisfactory hedonic scores up to 50% substituted sucrose (1). It is noncariogenic, nonglycemic, and contains few to no calories; therefore, it is safe for diabetics. Erythritol does not give humans a laxative effect, and it also has antioxidant properties. It is found abundantly in nature in fermented food, fruits, seaweed, and mushrooms (2). 3
In the study, sucrose was replaced with 25% and 50% inulin, as well as 25% and 50% erythritol, resulting in four batches of short-dough cookies, with an additional batch of 100% sucrose as the control (named 25SI, 50SI, 25SE, 50SE, and control, respectively). Other ingredients were flour, shortening, sucrose, milk powder, salt, sodium bicarbonate, ammonium hydrogen carbonate, and water. The sucrose or inulin/sucrose or erythritol/sucrose combinations were mixed with the reconstituted milk powder, baking soda, baking powder, and shortening in an electric mixer. After the flour was added, the dough rested for 10 minutes. The dough was then rolled out 10 mm thick and cut into rectangles 50 mm long and 30 mm wide, placed on a perforated tray, and placed in a conventional oven for 4 minutes at 175 C. For even browning, the trays were then rotated 180 and baked for another 4.5 minutes. After cooling, the cookies were stored in polypropylene bags for evaluation the following day. The process was the same for each sucrose/replacer mixture (1). In order to determine descriptive attributes appropriate for the consumer test, 9 trained analysts from ages 25-45 were brought in to sample cookies and suggested adjectives they thought best fit the cookies. The final attributes agreed upon were yellowness or toast color intensity, buttery or toast odor, porosity and convexity of the surface, hardness and sound intensity when broken, and matrix aeration after being broken in half. Flavor was to be defined as buttery, sweet, or toasty. In-mouth texture characteristics included hardness and sound at breaking on first bite, quality of the sound, hardness during chewing, sound length during chewing, and moisture mouth feel (1). The cookie samples were evaluated once more before the consumer test, their sensory attributes being scored. The 50SI cookie was determined to not be included in the consumer 4
testing because they were considered sensory intolerable due to the extreme hardness on the first bite with front teeth (1). 100 untrained consumers, ages 18-65, who claimed to regularly buy short-dough cookies took part in this study. They received four cookie samples (one each of 25SI, 25SE, 50SE, and control), which were coded with a random 3-digit number. They scored their liking of the cookies with a 9-point hedonic scale; if they scored the cookie with a 1, they extremely disliked it, and a score of 9 meant they extremely liked the cookie. The consumers were also scored for their overall acceptance, appearance, color, odor, texture, hardness, crispness, dry mouth feel, taste, sweetness, and butter taste of each cookie (1). The amount of moisture in the cookies was calculated by placing the cookies in an oven at 105 C so their weight would be consistent. Water activity was verified by measuring the humidity of the air and its equilibrium with the surface of the cookies (1). The acoustics of the cookies were an important part of this study. The cookies were placed in an isolated acoustic chamber with two different microphones. Cookies were placed on a flat surface over a slot and were then crushed with a wedge, meaning to simulate biting with the front teeth (1). The trained analyst panel of 9 people evaluated the consumer test scores for the 4 different cookie samples. Of all the observed attributes, 10 were statistically different. They were toast color intensity, surface porosity, manual hardness, matrix aeration, buttery or sweet flavor, hardness upon first bite, force of sound in mouth upon first bite, sound length during chewing, and moisture mouth feel. The cookie with the darkest toast color was the 50SE, then the control cookies, though there wasnt a significant distinction (1). 5
Usually, short-dough recipes do not call for enough water to dissolve all the sucrose; therefore, sucrose-crystal size after baking is an important factor in short-dough cookie manufacturing. In the study, crystallized sugar was found on the surface of the cookies. This causes holes in the cookies, called porosity. The cookies with the highest amount of porosity were the 50SE; the more sucrose that was replaced, the higher the porosity. This is due to the fact that erythritol is not water soluble, so it remained in solid crystals on the cookies surface. The erythritol cookies were also less flat than the inulin or control cookies (1). As far as hardness goes, the control and 25SI cookies were the hardest cookies, followed by 50SE and 25SE. Matrix aeration in the cookies (or how much air gets trapped within the cookie matrix) was an interesting factor to observe. The erythritol cookies were unable to trap air in the dough as well as the control and inulin cookies (1). The sweet and butter flavors (very important factors for consumer acceptance) were significantly unalike among the different cookies. The control, naturally, had the highest sweet and butter flavor scores (1). Erythritol is 60-80% as sweet as sucrose (2), and inulin is even less sweet, about 10% as sweet as sucrose (1). All the batches had the same amount of fat, so the sucrose apparently increased the buttery flavor. After the control batch, the best flavored cookies were followed by 25SE and 25SI, with 50SE tasting the least buttery (1). Hardness upon first bite was related to hardness when broken by hand, but the two had different results; it seemed to be harder to bite the cookie than it did to break it by hand. The control cookie was the hardest, and all the sucrose-replaced cookies were softer. The crust of the cookies was also harder when more sucrose was in the recipe. Moisture mouth feel was higher with the sucrose-replaced cookies than with the control, most likely due to the fact that they were 6
less hard. Similarly, the sound upon first bite and sound length while chewing was intense for the control and inulin cookies, but the erythritol cookies did not score as high in this category (1). Consumer acceptance is vital for a given product to be successful. Consumers accepted the 25SI cookie almost as much as the control. The least liked cookie was the 50SE (1). Replacing sucrose in a baked good affects not only the flavor, but the molecular structure as well. These changes affect the color and porosity of the surface, hardness, matrix aeration, sweet and buttery flavors, how loud the cookie is when bitten and chewed, and moisture mouth feel. The 25SI cookies were closest in all of these categories to the control cookies. Consumers least accepted the 50 SE cookie, which differed from the control or 25SI cookies in every way, except for its hardness and dry mouth feel. Compared to the control batch, the 25SI cookies were softer and more delicate; the cookies containing erythritol were harder to chew (1). A study at Hungkuang University found similar results, along with erythritol causing waxy and dry textures in Danish cookies (2). Therefore, erythritol is not the ideal sucrose replacer. Inulin, however, could replace sucrose up to 25% and gain much consumer acceptance. Further studies still must be conducted in order to test inulin in other reduced-sucrose or reduced-fat products; a study by Zahn, Pepke, and Rohm experimented with using inulin as a fat replacer in muffins, due to its ability to form a gel network when hydrated. They discovered that inulin cannot entirely replace fat (just like it cannot entirely replace sucrose) due to the unacceptable characteristics of the final baked good (3). However, if food scientists can perfect the inulin/sucrose/fat ratio, while still maintaining an acceptable number of kilocalories per serving, many delicious baked goods (and perhaps even candies) will be available on the market for people concerned with their weight, diabetes, or dental carries.
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References 1. Laguna L, Primo-Martin C, Salvador A, Sanz T. Inulin and Erythritol As Sucrose Replacers in Short-dough Cookies: Sensory, Fracture, and Acoustic Properties. Journal of Food Science. November 5, 2013; 78: S777-S784. 2. Lin S-D, Lee C-C, Mau J-L, Lin L, Chiou S-Y. Effect of Erythritol On Quality Characteristics of Reduced-Calorie Danish Cookies. Journal of Food Quality. 2010; 33: 14-26. 3. Zahn S, Pepke F, Rohm H. Effect of inulin as a fat replacer on texture and sensory properties of muffins. International Journal of Food Science & Technology. 2010; 45: 2530-2537. 4. Nesbitt M, Johnson N. Saccharum officinarum (sugar cane). Kew Royal Botanic Gardens website. 2010. Available at: http://www.kew.org/science-conservation/plants- fungi/saccharum-officinarum-sugar-cane. Accessed April 1, 2014. 5. Sugar and Desserts. American Diabetes Association website. 2014. Available at: http://www.diabetes.org/food-and-fitness/food/what-can-i-eat/understanding- carbohydrates/sugar-and-desserts.html. Accessed April 13, 2014.