Madrona, Vivien Alexandra C.

9/25/14
4Bio5
Genetics
THT-6

1.) A. Blomia tropicalis paramyosin allergen mRNA
B. Blomia tropicalis
C. Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
Acari; Acariformes; Sarcoptiformes; Astigmata; Glycyphagoidea;
Echimyopodidae; Blomia.
D. cDNA cloning
E. Int. Arch. Allergy Immunol. 126 (4), 286-293 (2001)
F. cDNA cloning and expression of Blo t 11, the Blomia tropicalis
allergen homologous to paramyosin
G. Ramos,J.D., Cheong,N., Lee,B.W. and Chua,K.Y.
H. 2628 bp
I. 1 atggcggctc gatcagcaaa gtatatgtat cagtccagtc gtgctggaca cggcggcgac
61 atttccatcg aatatggaac cgatttgggc gcactcaccc gacttgagga caagattcga
121 cttttgtccg aagatttgga aagcgaacgg gagcttcgac aacgtgttga acgagaaaag
181 tcggatatta ccgttcaatt gatgaacttg accgaacgtt tggaggagac cgaaggttcg
241 agcgaaagcg ttaccgagat gaacaagaaa cgagactcgg aattggctaa gcttcgaaag
301 ttgctcgaag atgtccatat ggaaagcgag gagactgctc accatttgag acaaaagcac
361 caagctgcca ttcaagagat gcaagatcaa ttggatcagg tgcaaaaggc aaagaacaag
421 tcggataagg agaagcaaaa gttccaagcc gaagttttcg aattgttggc tcaggttgag
481 actgccaaca aggacaagtt ggtcgctcag aagacggtag aaaagttgga atacaccgta
541 catgagttga acattaagat tgaagaaatc aatcgaactg ttgttgaggt taccgctcac
601 cgacaacgat tgtcgcaaga gaattcggaa ttgatcaagg aagttcatga atacaagatt
661 tcattggaca atgctaacca cttgaaggga caaattgcac aacaattgga ggacacccga
721 caccgattgg aggatgaaga acgaaaacga tcaagcttgg agaaccacgc ccacacattg
781 gaagttgaat tggaatcatt gaaggtacaa ttggaagagg aatctgaggc ccgattagaa
841 ttggaacgac aattgactaa agctaatggc gatgctgctt catggaaatc caaatacgaa
901 gccgaacttc aagctcacgt tgatgaggtt gaagaacttc gacgaaagat ggcccagaag
961 atttcggaat atggggaaca attggaagca ttgttgaaca aatgtagtgc attggaaaag
1021 caaaaggctc gactccaatc ggaggtagaa gttttgatca tggacttgga aaaggctacc
1081 gcacatgccc aggcattgga gaaacgcgtt tcccaattgg agaagatcaa cctcgacctc
1141 aagagcaagt tggaggaggt cagcatgttg ttggaacaaa ctcaaaagga tctccgtgtc
1201 aagattgcag acttgcagaa attgcaacac gagtacgaaa agttgcgaga ccaaaaggaa
1261 gcattggccc gtgagaacaa gaaactcgct gatgatttgg ctgaagctaa atctcagttg
1321 aatgacgccc accgacgaat ccatgaacaa gagatcgaga tcaaacgatt ggagaacgag
1381 cgagaagaat tggctgccgc atacaaggaa gctgaaacct tgcgaaaaca agaagaggca
1441 aagaatcaac gattgactgc cgagttggca cagacacgac acgactatga gaaacgattg
1501 gcacaaaagg aagaggagat tgaagctctt cgaaaacaat accaaattga aattgaacaa
1561 ttgaacatgc gattggctga ggctgaagct aaattgaaga ccgaggttgc acgactcaag
1621 aagaagtacc aagcacaaat cactgaattg gaattgtcgt tggatgccgc taataaggct
1681 aacatcgact tgcagaagac gatcaagaag caagctctcc aaatcactgg gcttcaagcc
1741 cattatgatg aggtacatcg tcaattgcaa caagctgttg atcaattggg tgtcactcaa
1801 cgacgatgcc aagcattgac cgccgaattg gaggagatgc gagtcaactt ggaacaagca
1861 cttcgtgcta aacgagctgc cgaacaaatg cacgaagagg cagttgtccg agtcaacgaa
1921 cttacaacca tcaatgtcaa cctcgcttcg gccaagagca aattggagac tgaattctct
1981 gctcttcaaa acgattacga tgaagtacac aaggaactcc gaatctctga cgaacgagtg
2041 caaaaactta ccattgaggt caagtcgaca aaggacttgc tcgaatcaga aacggaacgt
2101 gtcacaaaat tggaaacaat caagaaatca ttggaaaccg aagtacgaaa tctccaaatc
2161 cgaattgaag aagttgaggc taatgcattg gctggtggta aacgagtcat cgccaagttg
2221 gaatcgcgaa tccgtgatgt tgagattgag gttgaagaag aacgacgacg acacgcagaa
2281 actgaaaaga tgcttcgaaa gaaggatcat cgtgtcaagg aattgttgct tcaaaacgag
2341 gaggaccata aacaaattca attgttgcaa gaaatgtccg ataaattgaa cgaaaaggtc
2401 aaggtttaca aacgacaaat gcaagaacag gagggaatga gccaacaaaa cttgacccgt
2461 gtccgacgat tccaacgaga gttggaagcc gccgaagacc gagctgatca agctgaatcc
2521 aatttgtcgt tcatccgagc caaacatcga tcgtgggtca ccaccagtca agttcctggc
2581 ggtactcgac aagtctttgt cacggaacaa gagagctcga acttttaa

J.MAARSAKYMYQSSRAGHGGDISIEYGTDLGALTRLEDKIRLLSEDLESERELRQRVEREKSDITVQLMNLTER
LEETEGSSESVTEMNKKRDSELAKLRKLLEDVHMESEETAHHLRQKHQAAIQEMQDQLDQVQKAKNKSDKE
KQKFQAEVFELLAQVETANKDKLVAQKTVEKLEYTVHELNIKIEEINRTVVEVTAHRQRLSQENSELIKEVHEYKI
SLDNANHLKGQIAQQLEDTRHRLEDEERKRSSLENHAHTLEVELESLKVQLEEESEARLELERQLTKANGDAAS
WKSKYEAELQAHVDEVEELRRKMAQKISEYGEQLEALLNKCSALEKQKARLQSEVEVLIMDLEKATAHAQALE
KRVSQLEKINLDLKSKLEEVSMLLEQTQKDLRVKIADLQKLQHEYEKLRDQKEALARENKKLADDLAEAKSQLN
DAHRRIHEQEIEIKRLENEREELAAAYKEAETLRKQEEAKNQRLTAELAQTRHDYEKRLAQKEEEIEALRKQYQI
EIEQLNMRLAEAEAKLKTEVARLKKKYQAQITELELSLDAANKANIDLQKTIKKQALQITGLQAHYDEVHRQLQ
QAVDQLGVTQRRCQALTAELEEMRVNLEQALRAKRAAELTIEVKSTKDLLESETERVTKLETIKKSLETEVRNLQ
IRIEEVEANALAGGKRVIAKLESRIRDVEIEVEEERRRHAETEKMLRKKDHRVKELLLQNEEDHKQIQLLQEMSD
KLNEKVKVYKRQMQEQEGMSQQNLTRVRRFQRELEAAEDRADQAESNLSFIRAKHRSWVTTSQVPGGTRQ
VFVTEQESSNF

2.) A. Homo sapiens insulin (INS), transcript variant 1, mRNA
B. Homo sapiens
C. Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
D. cDNA clone encoding human preproinsuli
E. Nature
F. Nucleotide sequence of a cDNA clone encoding human preproinsulin
G. Tager,H., Given,B., Baldwin,D., Mako,M., Markese,J., Rubenstein,A.,
Olefsky,J., Kobayashi,M., Kolterman,O. and Poucher,R.
H. 469 bp
I. 1 agccctccag gacaggctgc atcagaagag gccatcaagc agatcactgt ccttctgcca
61 tggccctgtg gatgcgcctc ctgcccctgc tggcgctgct ggccctctgg ggacctgacc
121 cagccgcagc ctttgtgaac caacacctgt gcggctcaca cctggtggaa gctctctacc
181 tagtgtgcgg ggaacgaggc ttcttctaca cacccaagac ccgccgggag gcagaggacc
241 tgcaggtggg gcaggtggag ctgggcgggg gccctggtgc aggcagcctg cagcccttgg
301 ccctggaggg gtccctgcag aagcgtggca ttgtggaaca atgctgtacc agcatctgct
361 ccctctacca gctggagaac tactgcaact agacgcagcc cgcaggcagc cccacacccg
421 ccgcctcctg caccgagaga gatggaataa agcccttgaa ccagcaaaa

J. MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVEL
GGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN