Practical 1.

5: Retreiving Nucleotide Sequences

LOCUS
2000
DEFINITION
ACCESSION
VERSION
KEYWORDS
SOURCE
ORGANISM

AF091101

732 bp

mRNA

linear

ROD 08-JUN-

Mus musculus dUTPase mRNA, complete cds.

AF091101
AF091101.1 GI:8347091
.
Mus musculus (house mouse)
Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE
1 (bases 1 to 732)
AUTHORS
Kan,L., Varanasi,U., Zhu,Y., Qi,C. and Reddy,J.K.
TITLE
Mouse dUTPase cloning and genomic structural analysis
JOURNAL
Unpublished
REFERENCE
2 (bases 1 to 732)
AUTHORS
Kan,L., Varanasi,U., Zhu,Y., Qi,C. and Reddy,J.K.
TITLE
Direct Submission
JOURNAL
Submitted (08-SEP-1998) Pathology, Northwestern University, 303
E.Chicago Avenue, Chicago, IL 60611, USA
FEATURES
Location/Qualifiers
source
1..732
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
CDS
91..579
/codon_start=1
/product="dUTPase"
/protein_id="AAF74514.1"
/db_xref="GI:8347092"
/translation="MPCSEDAAAVSASKRARAEDGASLRFVRLSEHATTPTRGSARAA
GYDLFSAYDYTISPMEKAIVKTDIQIAVPSGCYGRVAPRSGLAVKHFIDVGAGVIDED
YRGNVGVVLFNFGKEKFEVKKGDRIAQLICERISYPDLEEVQTLDDTERGSGGFGSTG
KN"

ORIGIN
1
61
121
181
241
301
361
421
481
541
601
661
721

tcctgcgcgg
ttgctttctg
tctgcctcca
gagcacgcca
gcctatgatt
gctgtccctt
ttcatagatg
ctgtttaact
atctgtgagc
agaggctcag
ctgtttcaac
aggaaaggag
tgtaagtctg

tgggctcgtc
ctttttccac
agagggctcg
cgacgcccac
atacaatatc
ctgggtgcta
taggagctgg
ttgggaaaga
ggatttctta
gaggcttcgg
actggaaacc
acctcgatca
ca

caggcgggag
gcccgccgcc
agcggaggat
ccgcgggtcc
acccatggag
tggaagagta
tgtcatagac
gaagtttgaa
tccagactta
ctccaccggg
agaagctcta
catcacgttg

//

Ribosome Binding Site

Start Codon

Protein Coding Segment

Stop Codon

attttcggcg
atgccctgct
ggcgcttctc
gcgcgcgctg
aaagccatcg
gctccacgtt
gaggattaca
gtgaaaaaag
gaggaagtgc
aagaattaga
acttcggaag
gaacgattct

ggtaggctct
cggaagatgc
tgcgcttcgt
ccggctacga
tgaagacaga
ctggcttggc
gaggaaacgt
gtgatcggat
agaccctgga
actttgctgg
catttggtgt
gttccctggt

cgcacgctca
cgcggccgtc
gcggctctcg
cctattcagt
cattcagata
tgtaaagcac
tggggtcgtg
tgcgcagctc
tgacaccgag
aagtatctcg
tctaggatgc
tgaggtcgcc