The Urinary System

Christine Ward
Kidney Renal cortex

Renal Cortex:
Contains
nephrons,
convoluted
tubules, afferent
arterioles,
Interlobular veins,
cortical radiate
arteries.

Medulla

Kidney Leica 4x ,6.52ms

Christie Ward

Juxtamedullary Nephron

Cortical Nephron

Kidney Leica 10x 3.81ms

Christie Ward

Marty Dowers
Kidney Medullary region

Renal Cortex
Renal Medulla

Zeiss Axio, BF, 2.5x

Marty Dowers

Loop of Henle

Vasa Recta?

Zeiss Axio, BF, 40x

Marty Dowers

Collecting Ducts

Zeiss Axio, BF, 10x

Marty Dowers

Papillary Duct

Zeiss Axio, BF,63x

Marty Dowers

Angel McGee
Inner lining of the Urinary Bladder

Bladder
Transitional Epithelium

Basal Lamina

Lamina Propria
Lamina Propria
Smooth Muscle

Bladder
Dome Shaped Cell

Lamina Propria
Basal Lamina

Smooth Muscle

Sam Rodriguez
Urethra/Ureter

URETER
Adipose

} Mucosa
Smooth
Muscle

Lamina Propia

Transitional Epithelium

Function: Each kidney is connected to the urinary bladder by a ureter. The ureter
is a muscular tube. Its about 25-28cm long.

URETER

OVERLAY

King Burdett Jr.

Bladder

Umbrella Cells
Intermediate Region

Basal Cells
Lamina Propia

Submucosa
(dense irregular
connective tissue)

Smooth Muscle

Adventitia

Bladder

Name: KBBladderBF1,2,3,110414.jpg,
What: Monkey Bladder, Mag: 10x,
Phase: BF, Microscope: Leica, Exposure:
4.64ms, Gain:1, Saturation:1, Gamma:1,
Notes: The top layer of the bladder has a
columnar formatted epithelium.
Underneath this upholds a smooth
muscle with a combination of some e
sort of epithelium that clumps that look
like smooth muscle. As we scroll down
towards the bottom of this image you
may take into notice that there is a
transformation where the clumps
transform into this connective tissue
that appears to be some sort of
connective tissue. Also thus image
contains purple stained cells that have
seem to be associated with the
transformation that I have mentioned
previously in this image.

Bladder in Fluorescence

A
C

B
D

Name: KBBladderBF1,2,3,110414.jpg, What: Monkey Bladder, Mag: 20x, Phase: Cy3/DAPI/GFP, Microscope: Leica, Exposure: (Cy3: 278.8ms, DAPI:
3.5s, GFP: 2.4s) , Gain:1, Saturation:1, Gamma:1, Notes: The top layer of the bladder has a columnar formatted epithelium. Underneath this
upholds a smooth muscle with a combination of some e sort of epithelium that clumps that look like smooth muscle. As we scroll down towards the
bottom of this image you may take into notice that there is a transformation where the clumps transform into this connective tissue that appears to
be some sort of connective tissue. Also thus image contains purple stained cells that have seem to be associated with the transformation that I have
mentioned previously in this image. Except in fluorescence.

Female Reproductive System

primordial & primary follicles

Lindsay Estes

Primordial Follicles

TA
PF

Lindsay Estes
11/05/2014
Leica DM500, #2
Mammal
Graafian Follicles
Mag: 40x
Exposure: 3.3ms
Gain: 1.9x
Saturation: 1.0
Gamma: 0.63

cortex

Primordial follicles (PF): first stage of follicular development, composed of an immature

primary oocyte surrounded by a single layer of flattened follicular cells.
Tunica albuginea (TA): thick, fibrous connective tissue that encapsulates the ovary.
Cortex: the peripheral region of the ovary, below the TA, where ovarian follicles are found.
Lindsay Estes

Red

Green

Primordial
follicles

Blue (UV)

Overlay
Lindsay Estes

Primary Follicle
Lindsay Estes
11/05/2014
Leica DM500, #2
Ovary Corpora
Albicantia
Mag: 10x
Exposure: 931 s
Gain: 1.8x
Saturation: 1.0
Gamma: 0.75

Primary follicles: second stage of follicular development. Oocyte increases in size & follicular
cells develop from flat to cuboidal in shape.

Lindsay Estes

Primary Follicle

ZP
FC

Lindsay Estes
11/05/2014
Leica DM500, #2
Ovary Corpora
Albicantia
Mag: 40x
Exposure: 4.87ms
Gain: 1.8x
Saturation: 1.0
Gamma: 1.81

Oocyte (O): the egg increases in size & follicular cells develop from flat to cuboidal in shape.
Zona pellucida (ZP): the glycoprotein barrier surrounding the egg. Permits only one sperm to enter egg during
fertilization & is impenetrable to sperm after fertilization.
Follicular cells (FC): also called granulosa cells, they begin formation of stratified layers around the egg. At this
stage, they have not yet separated to form the fluid filled antrum cavity.

Lindsay Estes

Zona
pellucida

Red

Green

Blue (UV)

Overlay
Lindsay Estes

Yohannes Kidane
Vesicular follicle

Mature vesicular follicle

CR

A
SO
CO

Yohannes Kidane
11/15/2014
Leica DM500, #2
Ovary Corpora
Albicantia
Mag: 10x
Exposure: 2.97ms
Gain: 1.0x
Saturation: 1.0
Gamma: 1.0

ZP

Corona Radiata(CR): several layers of cells immediately surrounding the oocyte

Cumulus Oophorus (CO): group of cells anchored to the follicle wall that contains the oocyte.
Secondary Oocyte(SO): characterized by the formation an Antrum (A) a fluid filled space.
Zona pellucida (ZP): layer of glycoproteins between the oocyte and granulosa cells.
Yohannes Kidane

Lauri Zaker
Uterus

UTERUS

The uterus is divided into body (upper two-thirds) and cervix. The walls of theuterus are
composed of a mucosal layer, the endometrium, and a fibromuscular layer, the myometrium.
The peritoneal surface of the uterus is covered by a serosa.

Myometrium

The muscle fibres of the uterus form layers with preferred orientations of fibres (actually 4),
but this is very difficult to see in most preparations. The muscular tissue hypertrophies during
pregnancy, and GAP-junctions between cells become more frequent.

Endometrium
The endometrium consists of a simple columnar epithelium (ciliated cells and secretory cells)
and an underlying thick connective tissue stroma. The mucosa is invaginated to form many
simple tubular uterine glands. The glands extend through the entire thickness of the stroma.
The stromal cells of the endometrium are embedded in a network of reticular fibres. The
endometrium is subject to cyclic changes that result in menstruation. Only the mucosa of the
body of the uterus takes part in the menstrual cycle.

The endometrium can be divided into two zones based on their involvement in the changes
during the menstrual cycle: the basalis and the functionalis.

The basalis is not sloughed off during menstruation but functions as a regenerative zone for
the functionalis after its rejection.

The functionalis is the luminal part of the endometrium. It is sloughed off during every
menstruation and it is the site of cyclic changes in the endometrium. These cyclic changes are
divided into a number of phases: proliferative (or follicular), secretory (or luteal), and
menstrual.

Uterus

Perimetrium
Endometrium
Stratum Functionalis

Stratum Basalis

Myometrium
uterus in estrus phase 10x brightfield lauri zaker

The endometrium is simple columnar epithelium.

The myometrium is smooth muscle.

perimetrium and myometrium 10x

cropped (image j) brightfield lauri zaker

The perimetrium consists of connective tissue covered by a simple squamous

mesothelium.
endometrium 10x
cropped (image j) brightfield lauri zaker

Info from: https://web.duke.edu/histology/NormalBody/FemaleRepro/FemaleRepro.html

Uterus Estrus Fluorescents and Brightfield 10x

lauri zaker

Uterus
Progravid
(Luteal) Phase

uterus progravid phase 10x brightfield lauri zaker

Uterus
Follicular Phase

uterus follicular phase 10x brightfield lauri

Male Reproductive System

King Burdett Jr.

Testis

Testes

Leydig

Epididymis

Name: KBTestes1104144.jpg, What: Testes, Mag: 10x, Phase: BF, Microscope: Leica, Exposure: 48.2 ms, Gain:1, Saturation:1, Gamma:1, Notes: The
geography of this image consist of the testes zoomed in into the magnification of 10x. The centering of this image is this muscle epithelial looking
tissue in which I assumed that the sperm is being stored in. I also took into notice that there are some cells that are contained in these rubber bandlike epithelial formatted issue. This is also contained in the specimen that I assumed obtains the sperm.

Testes in Fluorescence

A
C

B
D

Name: KBTestes1104144.jpg, What: Testes, Mag: 10x, Phase: BF, Microscope: Leica, Exposure: 48.2 ms, Gain:1, Saturation:1, Gamma:1, Notes: The
geography of this image consist of the testes zoomed in into the magnification of 10x. He centering of this image is this muscle epithelial looking tissue
in which I assumed that the sperm is being stored in. I also took into notice that there are some cells that are contained in these rubber band-like
epithelial formatted issue. This is also contained in the specimen that I assumed obtains the sperm. Except in Fluorescence.

Seminiferous Tubules

KB.Seminmiferous110814.jpg, Mag: 10x, Phase: BF, Microscope: Leica, Exp: 2.24ms, Gain: 1, Sat: 1, Gamma: 1, Notes: The
geography of this image consist of seminiferous tubules. They contain some sort of epithelium that remains on the outside of the
tubule. In the center there are some epididymis and leydig cells.

Seminiferous Tubules in Fluorescence

A
C

B
D

KB.Seminmiferous110814.jpg, Mag: 20x, Phase: Cy3, GFP, DAPI, Microscope: Leica, Exp: 5.5s, Gain: 1, Sat: 1, Gamma: 1, Notes: The geography of this
image consist of seminiferous tubules. They contain some sort of epithelium that remains on the outside of the tubule. In the center there are some
epididymis and leydig cells.

Christine Ward
Epididymis

Spermatozoa

Smooth muscle
Pseudostratified columnar
epithelium

Epididymis Leica 10x 12.3 ms

Christie Ward

Lamina Propria

Basal cells
Principle cells

Stereocilia

Smooth muscle

Lumen of seminiferous tubules

Epididymis 40x
198.2ms

Ductus Deferens
The ductus deferens travels in a bundle of nerves
and blood vessels called the spermatic cord.It
goes up around the bladder and connects with
the seminal vesicles.Once they connect, it is called
the ejaculatory duct.

Lauri Zaker

Ductus Deferens

Pseudostratified
\pseudo ciliated
Columnar

Lamina Propria

outer
longitudinal
middle
circular

inner
longitudinal
Ductus deferens 4x lauri zaker

Ductus deferens 10 x lauri zaker

Prostate gland
Peter Werner

Prostate gland

epithelium glandular
duct
lumen

intralumenal secretion
(in glandular duct)

smooth muscle

alveolar connective tissue

2.5X objective, bright field

Prostate gland
intralumenal
secretion
lumen

glandular
duct

epithelium

alveolar connective tissue

smooth muscle

10X objective, bright field

Prostate gland

20X objective, bright field

Prostate gland

red

green

10X objective, fluorescence

Prostate gland

10X objective, fluorescence, red & green composite

Prostate gland
Yohannes Kidane

Yohannes Kidane
11/15/2014
Leica DM500, #2
Prostate gland
Mag: 10x
Exposure: 3.81ms
Gain: 1.0x
Saturation: 1.0
Gamma: 1.0

Glandular epithelium

The prostate is the largest accessory sex gland of the male reproductive system.
Mostly simple columnar and cuboidal epithelial cells but may have regions of
Pseudostratified columnar epithelium.

Yohannes Kidane

Yohannes Kidane
11/15/2014
Leica DM500, #2
Prostate gland
Mag: 10x
Exposure: 3.81ms
Gain: 1.0x
Saturation: 1.0
Gamma: 1.0

Pseudostratified epithelium

Basal Cells - small, round stem cells resting on the basement membrane.

Yohannes Kidane

Yohannes Kidane
11/15/2014
Leica DM500, #2
Prostate gland
Mag: 10x
Exposure: 3.81ms
Gain: 1.0x
Saturation: 1.0
Gamma: 1.0

Smooth muscle

Lamina Propria

Dense irregular connective tissue with interlaced bundles of smooth muscle.

Yohannes Kidane

Yohannes Kidane
11/15/2014
Leica DM500, #2
Prostate gland
Mag: 10x
Exposure: 3.81ms
Gain: 1.0x
Saturation: 1.0
Gamma: 1.0

Corpora Amalacea

Concretions of prostatic fluid in the lumen of some glands in older men.

Often have concentric lamellar appearance.
They gradually increase in number and size with age.

Yohannes Kidane