MOLECULAR

VIROLOGY

Fedik Abdul Rantam, DVM, Dr, Prof.

VIROLOGY AND IMMUNOLOGY
DEP OF MICROBIOLOGY, VET MED. AU
 MOLECULAR VIROLOGY

Virus are:
 Organisme subseluler with life cycle as
parasite in the cell
 Without metabolism activity out of cell
 Consist genetic materials RNA or DNA
 MOLECULAR VIROLOGY
 Consist some molecular to form virus
particle
 Molecular like Nucleotide, amino acid,
peptide, protein, enzyme
 Needed : transcription, translation,
assembling, virus release, epitope
 To build: structural protein and
nonstructural
 VIRUS COMPARTMENT

 Nucleic acid around protein consist more

lipid and sugar
 Virion function to transfer of genome in to
the cell for replication
 Receptor or effectors specific for
attachtment and transfer into the cell
 Protein is important role as molecule of
virus to design of their pathogenesis
 Bahan Dasar Virus

 Protein : structural protein and non

structural protein consist
nucleoprotein, capsid protein,
envelop protein and enzyme
 Amino acid: genetic code to protein
synthesis and production as new
virus (progeny)
 PROTEIN PRODUCE OF SINGLE GEN

 mRNA splicing
 Read through of stop codon
 Multiple star sites
 Overlapping genes
 Polyprotein cleavage
 Ribosomal frame shifting
 mRNA Splicing

 Proteinproduce through sequent

mechanism of part of intron in
precursor, who near with exon to
ligate again Example : Parvovirus,
adenovirus, herpesvirus (latent
infection of herpes virus)
 Readthrough of Stop Codons

 Proteinproduce of viral gene through

mechanism of reading stop codon,
who tRNA molecule to inside as
amino acid no more correlation with
polypeptide chain Example:
retrovirus
 Multiple Start Sites

 Protein produce of gene viral through

build new genetic material in some
places
 Example Herpes virus, hepadna
virus, papova virus, adeno virus
 Overlapping Genes

 Protein viral synthesis through mechanism

of single nucleotide sequence from single
strand with the function as two protein, but
needed two open reading frames. Example
AUGGCAAAUGGGCCUUUUACACAA
(AUG:MET)
 Example: hepadna virus, papova virus
 Polyprotein Cleavage

 Protein viral synthesis through

cleavage gene mechanism after
translation
 Example: Picorna virus
 Ribosomal Framshifting

 Protein viral gene synthesis through

build framshifting between upstream
and down stream
 Example : Retro virus
 Definition
 Swine Flu viral is taken
from name of influenza
cases which caused by
Orthomyxo virus which
be endemic in swine
population.

 Isolate of Swine flu were

classified by one of the
subtype virus from
influenza A genus
 PANDEMIC OF INFLUENZA

1918 - 1919 “SPANISH FLU” virus origin from

A (H1N1) avian waterfowl

STILL CIRCULATE TODAY

Virus have gen combination

1957 - 1958 “ASIAN FLU” from human dr virus influenza
A (H2N2) & avian waterfowl virus
HA, NA, PB1 = Avian influenza and swine

1968 - 1969 “HONG KONG FLU” Pandemic

A (H3N2) H1N1 ?
HA, NA
HA, PB1 = Avian
PANDEMIC PHASE OF INFLUENZA
(WHO)

H1N1 ?
 CHARACTERISTIC OF SWINE
INFLUENZA VIRUS
 Type: A, B, C
 Diameter 80 - 120 nm

 Pleomorphic, spherical,
filamentous particles

 Single-stranded RNA

 Segmented genome, 8
segments in A and B

 Hemagglutinin and
Neuraminidase on surface
of virion
 Information Genetik Changing

 Itshappen if: Increasing of segment

RNA count, so this condition cause of
low fidelity and mutation/ genetic
variation.This condition was called
with antigenic drift, if new genetic
variant was arise, so its be different
with old genetic, and this condition is
called with antigenic shift.
SEGMEN GENOM INFLUENZA VIRUS CHANGING IN MIX
INFECTION

Reassortment
(mixing vesel
in epitheleal
cells) Epitheleal cells

New Variant Virus

256 New Varian

 CRONOLOGIS OF EMERGING DISEASE
(Swine Flu)

Swine flu virus (Gripo

porcina)
Brote de H1
influenza
porcina 1976,
Ft. Dix
Avian waterfowl Influenza

H9 H7
H5 H5
H1
H3
H2
H1

1918 1957 1968 1977 1997 2003 2009

Influenza Influenza Influenza Influenza
Spanyol asia Hong Rusia 1998/9
H1N1 H2N2 Kong
H3N2
 EMERGENCY OF INFLUENZA

 Agent, that are necessary

global attention

 Influenza virus is causing

epidemy around at
1500’an, and pandemy is
knowing around at 1889.

 Pandemic accident at 1918

to1919, that caused people
dead around 20 million.
SWINE FLU DIFFERENT WITH HUMAN
FLU
 H1N1 influenza A virus or Swine flu
antigenicaly difference with H1N1
influenza A virus on human.

 No more protective if seasonal vaccine of

influenza was using for preventive of H1N1
influenza A virus infection.
Many H5N1-infected chickens

Reassort genes
Many H5N1-infected humans
Moderately efficient human-to-human
GENETIC OF INFLUENZA A VIRUS
SUBTIPE H5N1?
 GENETIC CODE
A C D E F G H I K L M AMIN
O
GCU UGU GAU GAA UUU GGU CAU AUU AAA UUA AUG
GCC UGC GAC GAG UUC GGC CAC AUC AAG UUG
ACID
GCA GGA AUA CUU CODE
GCG GGG CUC
CUA
CUG Codon

N P Q R S T V W Y STOP

AUU CCU CAA CGU UCU ACU GUU UGG UAU UAA
AAC CCC CAG CGC UCC ACC GUC UAC UAG
CCA CGA UCA ACA GUA UGA NUCLE
CCG CCG UCG ACG GUG O ACID
AGA AGU CODE
AGG AGC
EPITOP AND DESINFECTAN ?

Fedik AR. Fedik AR.

05 05
VIRUS AI ON FECES VIRUS AI AND
DESINFECTANT
 LOCALIZATION OF EPITOP
PROTEIN ?

Methode epitope
residue Analysis of Peptida
with 2-Demensi

Cristalography analysis Ag-Ab 15 - 22

complex
Sinthetic Peptida as antigenic probe 5 -8
Identification of critical residue on 3-5
peptide peptide
Peptida squence insert into 5-8
recombinan tprotein
Binding antipeptide antibody to 5-8
protein
Binding antiprotein Ab modificastion 3-5
protein mutation
Protection Ab bind residue epitope 5 - 10
 GENETIC CODE
A: Alanin M: Methionine
B: Aspargin/aspartic acid N: Aspargine
C: Cystein P: Proline
D: Aspartic acid
Q: Glutamine
E: Glutamic acid
R: Arginine
F: Phenylalanine
G: Glycine
S: Serine
H: Histidine T: Threonine
I: Isoleucine V: Valine
K: Lysine W: Tryptophane
L: Leucine Y: Tyrosine
Z: Glutamin/Glutamic acid
 GRSG MEI 2009

HA Polipasik/ FUSION PEPTIDE

Cleavage Site

#hewan ECPKYVKSNR LVLATGLRNS PQRERRRKKR GLFGAIAGFI EGGWQGMVDG [350]

#manusia .......... .......... ....S..... .......... .......... [350]

#hewan WYGYHHSNEQ GSGYAADKES TQKAIDGVTN KVNSIIDKMN TQFEAVGREF [400]

#manusia .......... .......... .......... .......... .......... [400]

#hewan NNLERRIENL NKKMEDGFLD VWTYNAELLV LMENERTLDF HDSNVKNLYD [450]

#manusia .......... .......... .......... .......... .......... [450]

#hewan KVRLQLRDNA KELGNGCFEF YHKCDNECME SIRNGTYNYP QYSEEARLKR [500]

#manusia .......... .......... .......... .......... .......... [500]

#hewan EEISGVKLES IGTYQILSIY STVASSLALA IMMAGLSLWM CSNGSLQCRICI

#manusia .......... .......... .......... ..I....... ............

Membrane domain
 I. A virus A/Indonesia/CDC1032N/2007 PB2
I. A virus A/Indonesia/CDC1032T/2007 PB2
I. A virus A/Chicken/Wajo/BBVM/2005 PB1
I. A virus A/Indonesia/CDC1032/2007 PB2
I. A virus A/duck/Parepare/BBVM/2005 PB1
I. A virus A/Indonesia/CDC938/2006 PB2
I. A virus A/chicken/Magetan/BBVW/200...
I. A virus A/Indonesia/CDC1031/2007 PB2 I. A virus (A/chicken/Gunung A/chicke...

I. A virus A/Indonesia/CDC1031T/2007 PB2 I. A virus A/chicken/Indonesia/CDC25/...

I. A virus A/chicken/Indonesia/CDC24/...
I. A virus A/Indonesia/CDC1031T2/2007...
I. A virus A/Indonesia/CDC390/2006 PB...

PB2 and PB1

I. A virus A/Indonesia/CDC1031RE2/200... I. A virus A/Indonesia/CDC370/2006 PB...
I. A virus A/Indonesia/CDC887/2006 PB2 I. A virus A/Indonesia/CDC370E/2006 P...

I. A virus A/Indonesia/CDC523E/2006 PB2 I. A virus A/Indonesia/CDC370P/2006 P...

I. A virus A/Indonesia/CDC370T/2006 P...
I. A virus A/Indonesia/CDC523T/2006 PB2
I. A virus A/Indonesia/CDC7/2005 PB1+...
I. A virus A/Indonesia/CDC523/2006 PB2 I. A virus A/Indonesia/CDC184/2005 PB...
I. A virus A/Indonesia/CDC699/2006 PB2 I. A virus A/Indonesia/CDC194P/2005 P...

I. A virus A/Indonesia/CDC287E/2005 PB2 I. A virus (A/Indonesia/5/2005(H5N1))...

I. A virus A/Indonesia/CDC623/2006 PB...
I. A virus A/Indonesia/CDC287T/2005 PB2
I. A virus A/Indonesia/CDC623E/2006 P...
I. A virus A/Indonesia/CDC582/2006 PB2 I. A virus A/Indonesia/CDC624/2006 PB...
I. A virus A/Indonesia/CDC644T/2006 PB2 I. A virus A/Indonesia/CDC624E/2006 P...
I. A virus A/Chicken/Yogjakarta/BBVet...
I. A virus A/Indonesia/CDC644/2006 PB2
I. A virus A/quail/Boyolali/BPPV4/200...
I. A virus A/Indonesia/CDC634/2006 PB2
I. A virus A/chicken/Purworejo/BBVW/2...
I. A virus A/Indonesia/CDC634P/2006 PB2 I. A virus A/chicken/Jembrana/BPPV6/2...

I. A virus A/Indonesia/CDC634T/2006 PB2 I. A virus A/quail/Tasikmalaya/BPPV4/...

I. A virus A/Ck/Indonesia/BL/2003 PB1
I. A virus A/Indonesia/CDC742/2006 PB2
I. A virus A/chicken/Ngawi/BPPV4/2004...
I. A virus A/Indonesia/CDC835/2006 PB2
I. A virus A/chicken/Pekalongan/BPPV4...
I. A virus A/Indonesia/CDC610/2006 PB2 I. A virus A/Chicken/Wonosobo/BPPV4/2...

I. A virus A/Indonesia/CDC739/2006 PB2 I. A virus A/Ck/Indonesia/PA/2003 PB1

I. A virus (A/chicken/Kulon A/Chicken...
I. A virus A/Indonesia/CDC759/2006 PB2
I. A virus A/Ck/Indonesia/5/2004 PB1
I. A virus A/Indonesia/CDC669/2006 PB2
I. A virus A/Ck/Indonesia/2A/2003 PB1
I. A virus A/Indonesia/CDC669P/2006 PB2 I. A virus A/chicken/Sragen/BPPV4/200...

I. A virus A/Indonesia/CDC940/2006 PB2 I. A virus A/Chicken/Bantul/BBVet-I/2...

I. A virus (A/chicken/Bangli A/chicke...
I. A virus A/Indonesia/CDC836/2006 PB2
I. A virus A/chicken/Mangarai-NTT/BPP...
I. A virus A/Indonesia/CDC836T/2006 PB2 I. A virus A/chicken/Kupang-2-NTT/BPP...
I. A virus A/feline/Indonesia/CDC1/20... I. A virus A/chicken/Kupang-3-NTT/BPP...

I. A virus A/Indonesia/CDC326/2006 PB2 I. A virus A/Dk/Indonesia/MS/2004 PB1

I. A virus A/chicken/Malang/BBVet-IV/...
I. A virus A/Indonesia/CDC326T/2006 PB2
I. A virus A/chicken/Purwakarta/BBVet...
I. A virus A/Indonesia/CDC329/2006 PB2 I. A virus A/chicken/Pangkalpinang/BP...
I. A virus A/Indonesia/CDC326N/2006 PB2 I. A virus A/turkey/Kedaton/BPPV3/200...

FILOGENETIK AI
I. A virus A/Chicken/Salatiga/BBVet-I...
I. A virus A/Indonesia/CDC326N2/2006 PB2
I. A virus (A/chicken/Tebing A/chicke...
I. A virus A/Indonesia/CDC357/2006 PB2
I. A virus A/chicken/Tarutung/BPPVI/2...
I. A virus A/Indonesia/CDC292N/2005 PB2 I. A virus (A/chicken/Deli A/chicken/...
I. A virus A/Indonesia/CDC292T/2005 PB2 I. A virus A/chicken/Dairi/BPPVI/2005...

ANIMAN AND HUMAN

I. A virus A/chicken/Simalanggang/BPP...
I. A virus A/Indonesia/CDC1046/2007 PB2
I. A virus A/Indonesia/CDC625/2006 PB...
I. A virus A/Indonesia/CDC1047/2007 PB2
I. A virus A/Indonesia/CDC625L/2006 P...
I. A virus A/Indonesia/CDC623/2006 PB2 I. A virus A/Indonesia/CDC594/2006 PB...

I. A virus A/Indonesia/CDC623E/2006 PB2 I. A virus A/Indonesia/CDC595/2006 PB...

I. A virus A/Indonesia/CDC596/2006 PB...
I. A virus A/Indonesia/CDC624/2006 PB2
I. A virus A/Indonesia/CDC599/2006 PB...
I. A virus A/Indonesia/CDC624E/2006 PB2
I. A virus A/Indonesia/CDC599N/2006 P...
I. A virus A/Indonesia/CDC390/2006 PB2 I. A virus A/Indonesia/CDC597/2006 PB...

I. A virus A/Indonesia/CDC370T/2006 PB2 I. A virus A/Ck/Indonesia/4/2004 PB1

I. A virus A/chicken/Kupang-1-NTT/BPP...
I. A virus A/Indonesia/CDC370E/2006 PB2
I. A virus A/Indonesia/CDC287E/2005 P...
I. A virus A/Indonesia/CDC370/2006 PB2 I. A virus A/Indonesia/CDC287T/2005 P...
I. A virus A/Indonesia/CDC370P/2006 PB2 I. A virus A/Indonesia/CDC326N/2006 P...

I. A virus A/Indonesia/CDC194P/2005 PB2 I. A virus A/Indonesia/CDC326N2/2006 ...

I. A virus A/Indonesia/CDC326/2006 PB...
I. A virus (A/Indonesia/5/2005(H5N1))...
I. A virus A/Indonesia/CDC326T/2006 P...
I. A virus A/Indonesia/CDC7/2005 PB2 I. A virus A/Indonesia/CDC329/2006 PB...
I. A virus A/Indonesia/CDC184/2005 PB2 I. A virus A/Indonesia/CDC940/2006 PB...
I. A virus A/Indonesia/CDC759/2006 PB...
I. A virus A/chicken/Magetan/BBVW/200...
I. A virus A/Indonesia/CDC836/2006 PB...
I. A virus (A/chicken/Gunung A/chicke...
I. A virus A/Indonesia/CDC836T/2006 P...
I. A virus A/duck/Parepare/BBVM/2005 PB2 I. A virus A/Indonesia/CDC292N/2005 P...
I. A virus A/chicken/Indonesia/CDC25/... I. A virus A/Indonesia/CDC292T/2005 P...
I. A virus A/Indonesia/CDC742/2006 PB...
I. A virus A/chicken/Indonesia/CDC24/...
I. A virus A/feline/Indonesia/CDC1/20...
I. A virus A/quail/Yogjakarta/BBVet-I...
I. A virus A/Indonesia/CDC357/2006 PB...
I. A virus A/chicken/Purworejo/BBVW/2... I. A virus A/Indonesia/CDC699/2006 PB...

I. A virus (A/chicken/Bangli A/chicke(2) I. A virus A/Indonesia/CDC523T/2006 P...

I. A virus A/Indonesia/CDC523/2006 PB...
I. A virus A/chicken/Kupang-3-NTT/BPP...
I. A virus A/Indonesia/CDC523E/2006 P...
I. A virus A/chicken/Kupang-2-NTT/BPP...
I. A virus A/Indonesia/CDC644/2006 PB...
I. A virus A/chicken/Mangarai-NTT/BPP... I. A virus A/Indonesia/CDC739/2006 PB...

I. A virus (A/chicken/Bangli A/chicke... I. A virus A/Indonesia/CDC669P/2006 P...

I. A virus A/Indonesia/CDC669/2006 PB...
I. A virus (A/chicken/Kulon A/chicken...
I. A virus A/Indonesia/CDC610/2006 PB...
I. A virus (A/chicken/Kulon A/chicken(2) I. A virus A/Indonesia/CDC835/2006 PB...
I. A virus A/Dk/Indonesia/MS/2004 PB2 I. A virus A/Indonesia/CDC644T/2006 P...

I. A virus A/Ck/Indonesia/BL/2003 PB2 I. A virus A/Indonesia/CDC582/2006 PB...

I. A virus A/Indonesia/CDC634/2006 PB...
I. A virus A/chicken/Purwakarta/BBVet...
I. A virus A/Indonesia/CDC634P/2006 P...
I. A virus A/Ck/Indonesia/PA/2003 PB2 I. A virus A/Indonesia/CDC634T/2006 P...
I. A virus (A/chicken/Tebing A/chicke... I. A virus A/Indonesia/CDC887/2006 PB...
I. A virus A/Indonesia/CDC938/2006 PB...
I. A virus A/chicken/Tarutung/BPPVI/2...
I. A virus A/Indonesia/CDC938E/2006 P...
I. A virus (A/chicken/Deli A/chicken/...
I. A virus A/Indonesia/CDC1031/2007 P...
I. A virus A/chicken/Simalanggang/BPP... I. A virus A/Indonesia/CDC1031RE2/200...
I. A virus A/chicken/Dairi/BPPVI/2005... I. A virus A/Indonesia/CDC1031T2/2007...
I. A virus A/Indonesia/CDC1046T/2007 ...
I. A virus A/Indonesia/CDC599N/2006 PB2
I. A virus A/Indonesia/CDC1047/2007 P...
I. A virus A/Indonesia/CDC599/2006 PB2
I. A virus A/Indonesia/CDC1047S/2007 ...
I. A virus A/Indonesia/CDC625/2006 PB2 I. A virus A/Indonesia/CDC1032/2007 P...

I. A virus A/Indonesia/CDC625L/2006 PB2 I. A virus A/Indonesia/CDC1032N/2007 ...

I. A virus A/Indonesia/CDC594/2006 PB2
I. A virus A/Indonesia/CDC595/2006 PB2
I. A virus A/Indonesia/CDC596/2006 PB2
I. A virus A/Indonesia/CDC1032T/2007 ...
I. A virus A/Indonesia/CDC1046/2007 P...
0.001

I. A virus A/Indonesia/CDC597/2006 PB2

0.002
 I. A virus A/Indonesia/CDC357/2006 PA
I. A virus A/Indonesia/CDC739/2006 HA
I. A virus A/feline/Indonesia/CDC1/20...
I. A virus A/Indonesia/CDC329/2006 PA
I. A virus A/Indonesia/CDC836/2006 HA
I. A virus A/Indonesia/CDC326N2/2006 PA I. A virus A/Indonesia/CDC759/2006 HA
I. A virus A/Indonesia/CDC287E/2005 PA
I. A virus A/Indonesia/CDC669/2006 HA
I. A virus A/Indonesia/CDC287T/2005 PA
I. A virus A/Indonesia/CDC326N/2006 PA
I. A virus A/Indonesia/CDC940/2006 HA

PA and HA
I. A virus A/Indonesia/CDC326/2006 PA I. A virus A/Indonesia/CDC835/2006 HA
I. A virus A/Indonesia/CDC326T/2006 PA
I. A virus A/Indonesia/CDC610/2006 HA
I. A virus A/Indonesia/CDC292N/2005 PA
I. A virus A/Indonesia/CDC292T/2005 PA
I. A virus A/Indonesia/CDC582/2006 HA
I. A virus A/Indonesia/CDC742/2006 PA I. A virus A/Indonesia/CDC644/2006 HA
I. A virus A/Indonesia/CDC610/2006 PA
I. A virus A/Indonesia/CDC634/2006 HA
I. A virus A/Indonesia/CDC835/2006 PA
I. A virus A/Indonesia/CDC836/2006 PA I. A virus A/Indonesia/CDC699/2006 HA
I. A virus A/Indonesia/CDC836T/2006 PA I. A virus A/Indonesia/CDC523/2006 HA
I. A virus A/Indonesia/CDC940/2006 PA
I. A virus A/Indonesia/CDC1031/2007 HA
I. A virus A/Indonesia/CDC669/2006 PA
I. A virus A/Indonesia/CDC669P/2006 PA I. A virus A/Indonesia/CDC887/2006 HA
I. A virus A/Indonesia/CDC739/2006 PA I. A virus A/Indonesia/CDC938/2006 HA
I. A virus A/Indonesia/CDC759/2006 PA
I. A virus A/Indonesia/CDC1032/2007 HA
I. A virus A/Indonesia/CDC634P/2006 PA
I. A virus A/Indonesia/CDC634T/2006 PA I. A virus A/Indonesia/CDC1047/2007 HA
I. A virus A/Indonesia/CDC634/2006 PA I. A virus A/Indonesia/CDC1046/2007 HA
I. A virus A/Indonesia/CDC644T/2006 PA
I. A virus A/Indonesia/CDC390/2006 HA
I. A virus A/Indonesia/CDC644/2006 PA
I. A virus A/Indonesia/CDC582/2006 PA I. A virus A/Indonesia/CDC370/2006 HA
I. A virus A/Indonesia/CDC523T/2006 PA I. A virus A/Indonesia/CDC623/2006 HA
I. A virus A/Indonesia/CDC523/2006 PA
I. A virus A/Indonesia/CDC624/2006 HA
I. A virus A/Indonesia/CDC699/2006 PA
I. A virus A/Indonesia/CDC523E/2006 PA I. A virus A/Indonesia/CDC292T/2005 HA
I. A virus A/Indonesia/CDC1031T/2007 PA I. A virus A/Indonesia/CDC742/2006 HA
I. A virus A/Indonesia/CDC1031RE2/200...
I. A virus A/Indonesia/CDC357/2006 HA
I. A virus A/Indonesia/CDC887/2006 PA
I. A virus A/Indonesia/CDC938/2006 PA I. A virus A/feline/Indonesia/CDC1/20...
I. A virus A/Indonesia/CDC1032N/2007 PA I. A virus A/Indonesia/CDC329/2006 HA
I. A virus A/Indonesia/CDC1032T/2007 PA
I. A virus A/Indonesia/CDC1032/2007 PA
I. A virus A/Indonesia/CDC1046/2007 PA
FILOGENETIK AI I. A virus A/Indonesia/CDC326/2006 HA
I. A virus (A/Indonesia/5/05(H5N1)) A...

ANIMAN AND HUMAN

I. A virus A/Indonesia/CDC1046T/2007 PA I. A virus A/Indonesia/CDC194P/2005 HA
I. A virus A/Indonesia/CDC1047/2007 PA
I. A virus A/Indonesia/CDC184/2005 HA
I. A virus A/Indonesia/CDC1047S/2007 PA
I. A virus A/Indonesia/CDC390/2006 PA I. A virus A/Indonesia/CDC7/2005 HA
I. A virus A/Indonesia/CDC370P/2006 PA I. A virus A/Chicken/Wajo/BBVM/2005 HA
I. A virus A/Indonesia/CDC370/2006 PA
I. A virus A/duck/Parepare/BBVM/2005 HA
I. A virus A/Indonesia/CDC370E/2006 PA
I. A virus A/Indonesia/CDC623/2006 PA I. A virus (A/chicken/Gunung A/chicke...
I. A virus A/Indonesia/CDC623E/2006 PA I. A virus A/chicken/Magetan/BBVW/200...
I. A virus A/Indonesia/CDC624/2006 PA
I. A virus A/chicken/Purworejo/BBVW/2...
I. A virus A/Indonesia/CDC624E/2006 PA
I. A virus (A/Indonesia/5/2005(H5N1))... C Q/Yogjakarta/BBVet...
I. A virus A/Indonesia/CDC7/2005 PA I. A virus A/quail/Boyolali/BPPV4/200...
I. A virus A/Indonesia/CDC184/2005 PA
I. A virus (A/chicken/Kulon A/Chicken...
I. A virus A/chicken/Magetan/BBVW/200...
I. A virus (A/chicken/Gunung A/chicke... I. A virus A/chicken/Kupang-1-NTT/BPP...
I. A virus A/duck/Parepare/BBVM/2005 PA I. A virus A/Indonesia/CDC597/2006 HA
I. A virus A/chicken/Indonesia/CDC25/...
I. A virus A/Indonesia/CDC625/2006 HA
I. A virus A/chicken/Indonesia/CDC24/...
I. A virus A/Chicken/Yogjakarta/BBVet... I. A virus A/Indonesia/CDC594/2006 HA
I. A virus A/quail/Yogjakarta/BBVet-I... I. A virus A/Chicken/Salatiga/BBVet-I...
I. A virus A/chicken/Purworejo/BBVW/2...
I. A virus A/chicken/Pangkalpinang/BP...
I. A virus A/chicken/Kupang-2-NTT/BPP...
I. A virus A/chicken/Kupang-3-NTT/BPP... I. A virus A/turkey/Kedaton/BPPV3/200...
I. A virus A/chicken/Mangarai-NTT/BPP... I. A virus A/chicken/Indonesia/R60/05...
I. A virus A/Dk/Indonesia/MS/2004 PA
I. A virus A/chicken/Ngawi/BPPV4/2004 HA
I. A virus A/Ck/Indonesia/BL/2003 PA
I. A virus (A/chicken/Kulon A/Chicken... C Bali NTT+
I. A virus (A/chicken/Bangli A/chicke... I. A virus A/Ck/Indonesia/5/2004 HA
I. A virus A/chicken/Malang/BBVet-IV/...
I. A virus A/Ck/Indonesia/2A/2003 HA
I. A virus A/chicken/Purwakarta/BBVet...
I. A virus A/Ck/Indonesia/PA/2003 PA I. A virus A/Dk/Indonesia/MS/2004 HA
I. A virus A/Chicken/Salatiga/BBVet-I... I. A virus A/chicken/Sragen/BPPV4/200...
I. A virus A/chicken/Simalanggang/BPP...
I. A virus A/Ck/Indonesia/BL/2003 HA
I. A virus (A/chicken/Deli A/chicken/...
I. A virus A/chicken/Tarutung/BPPVI/2... C Malang Purwakarta
I. A virus (A/chicken/Tebing A/chicke... I. A virus A/chicken/Pekalongan/BPPV4...
I. A virus A/chicken/Dairi/BPPVI/2005 PA
I. A virus A/quail/Tasikmalaya/BPPV4/...
I. A virus A/Indonesia/CDC595/2006 PA
I. A virus A/Indonesia/CDC625/2006 PA I. A virus A/chicken/Indonesia/7/2003 HA
I. A virus A/Indonesia/CDC625L/2006 PA I. A virus A/Chicken/Wonosobo/BPPV4/2...
I. A virus A/Indonesia/CDC599N/2006 PA
I. A virus A/chicken/Indonesia/11/200...
I. A virus A/Indonesia/CDC594/2006 PA
I. A virus A/Indonesia/CDC596/2006 PA I. A virus A/Ck/Indonesia/PA/2003 HA
I. A virus A/Indonesia/CDC597/2006 PA I. A virus A/Ck/Indonesia/4/2004 HA
I. A virus A/Indonesia/CDC599/2006 PA

0.002 0.002
 I. A virus A/Indonesia/CDC610/2006 NA
I. A virus A/Indonesia/CDC836/2006 NP I. A virus A/Indonesia/CDC835/2006 NA
I. A virus A/Indonesia/CDC836T/2006 NP I. A virus A/Indonesia/CDC287E/2005 NA
I. A virus A/Indonesia/CDC940/2006 NP I. A virus A/Indonesia/CDC292N/2005 NA
I. A virus A/Indonesia/CDC759/2006 NP I. A virus A/Indonesia/CDC292T/2005 NA

I. A virus A/Indonesia/CDC669/2006 NP I. A virus A/Indonesia/CDC326T/2006 NA

I. A virus A/Indonesia/CDC739/2006 NP I. A virus A/Indonesia/CDC329/2006 NA
I. A virus A/Indonesia/CDC326N/2006 NA

NP and NA
I. A virus A/Indonesia/CDC669P/2006 NP
I. A virus A/Indonesia/CDC582/2006 NP I. A virus A/Indonesia/CDC326/2006 NA

I. A virus A/Indonesia/CDC634/2006 NP I. A virus A/Indonesia/CDC742/2006 NA

I. A virus A/Indonesia/CDC634P/2006 NP I. A virus A/Indonesia/CDC357/2006 NA

I. A virus A/Indonesia/CDC634T/2006 NP I. A virus A/feline/Indonesia/CDC1/20...

I. A virus A/Indonesia/CDC644T/2006 NP I. A virus A/Indonesia/CDC836/2006 NA

I. A virus A/Indonesia/CDC644/2006 NP I. A virus A/Indonesia/CDC940/2006 NA

I. A virus A/Indonesia/CDC835/2006 NP I. A virus A/Indonesia/CDC836T/2006 NA

I. A virus A/Indonesia/CDC610/2006 NP I. A virus A/Indonesia/CDC669/2006 NA

I. A virus A/Indonesia/CDC1031/2007 NP I. A virus A/Indonesia/CDC669P/2006 NA

I. A virus A/Indonesia/CDC1031T2/2007 NP I. A virus A/Indonesia/CDC739/2006 NA

I. A virus A/Indonesia/CDC1031T/2007 NP I. A virus A/Indonesia/CDC759/2006 NA

I. A virus A/Indonesia/CDC1031RE2/200... I. A virus A/Indonesia/CDC1046/2007 NA

I. A virus A/Indonesia/CDC523/2006 NP I. A virus A/Indonesia/CDC1046T/2007 NA

I. A virus A/Indonesia/CDC523E/2006 NP I. A virus A/Indonesia/CDC1047/2007 NA

I. A virus A/Indonesia/CDC523T/2006 NP I. A virus A/Indonesia/CDC1047S/2007 NA

I. A virus A/Indonesia/CDC623/2006 NA
I. A virus A/Indonesia/CDC699/2006 NP
I. A virus A/Indonesia/CDC624/2006 NA
I. A virus A/Indonesia/CDC287E/2005 NP
I. A virus A/Indonesia/CDC624E/2006 NA
I. A virus A/Indonesia/CDC938/2006 NP
I. A virus A/Indonesia/CDC370/2006 NA
I. A virus A/Indonesia/CDC938E/2006 NP
I. A virus A/Indonesia/CDC370E/2006 NA
I. A virus A/Indonesia/CDC1032/2007 NP
I. A virus A/Indonesia/CDC390/2006 NA
I. A virus A/Indonesia/CDC1032N/2007 NP
I. A virus A/Indonesia/CDC634P/2006 NA
I. A virus A/Indonesia/CDC1032T/2007 NP
I. A virus A/Indonesia/CDC634T/2006 NA
I. A virus A/Indonesia/CDC887/2006 NP
I. A virus A/Indonesia/CDC634/2006 NA
I. A virus A/Indonesia/CDC292N/2005 NP
I. A virus A/Indonesia/CDC644T/2006 NA
I. A virus A/feline/Indonesia/CDC1/20...
I. A virus A/Indonesia/CDC644/2006 NA
I. A virus A/Indonesia/CDC742/2006 NP
I. A virus A/Indonesia/CDC582/2006 NA
I. A virus A/Indonesia/CDC357/2006 NP
I. A virus A/Indonesia/CDC699/2006 NA
I. A virus A/Indonesia/CDC326T/2006 NP
I. A virus A/Indonesia/CDC523E/2006 NA
I. A virus A/Indonesia/CDC329/2006 NP
I. A virus A/Indonesia/CDC523T/2006 NA

FILOGENETIK AI
I. A virus A/Indonesia/CDC326/2006 NP
I. A virus A/Indonesia/CDC523/2006 NA
I. A virus A/Indonesia/CDC326N/2006 NP
I. A virus A/Indonesia/CDC1031T2/2007 NA
I. A virus A/Indonesia/CDC292T/2005 NP
I. A virus A/Indonesia/CDC1031RE2/200...
I. A virus A/Indonesia/CDC1046/2007 NP

ANIMAN AND HUMAN

I. A virus A/Indonesia/CDC1031T/2007 NA
I. A virus A/Indonesia/CDC1046T/2007 NP
I. A virus A/Indonesia/CDC1031/2007 NA
I. A virus A/Indonesia/CDC1047/2007 NP
I. A virus A/Indonesia/CDC938/2006 NA
I. A virus A/Indonesia/CDC1047S/2007 NP
I. A virus A/Indonesia/CDC938E/2006 NA
I. A virus A/Indonesia/CDC390/2006 NP
I. A virus A/Indonesia/CDC887/2006 NA
I. A virus A/Indonesia/CDC370/2006 NP
I. A virus A/Indonesia/CDC1032/2007 NA
I. A virus A/Indonesia/CDC370E/2006 NP
I. A virus A/Indonesia/CDC1032N/2007 NA
I. A virus A/Indonesia/CDC623/2006 NP
I. A virus A/Indonesia/CDC1032T/2007 NA
I. A virus A/Indonesia/CDC623E/2006 NP
I. A virus A/Indonesia/CDC7/2005 NA
I. A virus A/Indonesia/CDC624/2006 NP
I. A virus A/Indonesia/CDC184/2005 NA
I. A virus A/Indonesia/CDC624E/2006 NP
I. A virus A/Indonesia/CDC194P/2005 NA
I. A virus A/Indonesia/CDC194P/2005 NP
I. A virus A/chicken/Indonesia/CDC25/...
I. A virus (A/Indonesia/5/2005(H5N1))...
I. A virus A/chicken/Indonesia/CDC24/...
I. A virus A/chicken/Magetan/BBVW/200...
I. A virus (A/chicken/Gunung A/chicke...
I. A virus (A/chicken/Gunung A/chicke...
I. A virus A/duck/Parepare/BBVM/2005 NA
I. A virus A/Indonesia/CDC7/2005 NP
I. A virus A/quail/Tasikmalaya/BPPV42...
I. A virus A/Indonesia/CDC184/2005 NP
I. A virus A/chicken/Indonesia/7/2003 NA
I. A virus A/chicken/Indonesia/CDC25/...
I. A virus A/quail/Yogjakarta/BBVet-I...
I. A virus A/Chicken/Wajo/BBVM/2005 NP
I. A virus A/Ck/Indonesia/PA/2003 NA
I. A virus A/duck/Parepare/BBVM/2005 NP
I. A virus A/chicken/Indonesia/11/200...
I. A virus A/chicken/Pekalongan/BPPV4...
I. A virus A/chicken/Indonesia/R134/0...
I. A virus A/Dk/Indonesia/MS/2004 NP
I. A virus A/chicken/Indonesia/R60/05...
I. A virus A/Ck/Indonesia/5/2004 NP
I. A virus A/chicken/Purwakarta/BBVet...
I. A virus A/chicken/Ngawi/BPPV4/2004 NP
I. A virus A/chicken/Purworejo/BBVW/2...
I. A virus A/Ck/Indonesia/2A/2003 NP
I. A virus A/Ck/Indonesia/BL/2003 NA
I. A virus A/chicken/Jembrana/BPPV6/2...
I. A virus (A/chicken/Kulon A/chicken...
I. A virus A/chicken/Sragen/BPPV4/200... I. A virus A/chicken/Kupang-1-NTT/BPP...
I. A virus A/Ck/Indonesia/BL/2003 NP I. A virus A/Ck/Indonesia/5/2004 NA
I. A virus A/quail/Tasikmalaya/BPPV4/... I. A virus A/Dk/Indonesia/MS/2004 NA
I. A virus A/Ck/Indonesia/4/2004 NP I. A virus A/Ck/Indonesia/2A/2003 NA
I. A virus A/Chicken/Wonosobo/BPPV4/2... I. A virus (A/chicken/Bangli A/chicke...
I. A virus A/chicken/Purworejo/BBVW/2... I. A virus A/chicken/Mangarai-NTT/BPP...
I. A virus A/Ck/Indonesia/PA/2003 NP I. A virus A/chicken/Kupang-2-NTT/BPP...
I. A virus A/quail/Boyolali/BPPV4/200... I. A virus A/chicken/Kupang-3-NTT/BPP...
I. A virus A/chicken/Pangkalpinang/BP... I. A virus A/turkey/Kedaton/BPPV3/200...
I. A virus A/chicken/Simalanggang/BPP... I. A virus A/Chicken/Salatiga/BBVet-I...
I. A virus A/chicken/Dairi/BPPVI/2005 NP I. A virus A/chicken/Indonesia/77/200...
I. A virus (A/chicken/Tebing A/chicke... I. A virus (A/chicken/Tebing A/chicke...
I. A virus A/chicken/Tarutung/BPPVI/2... I. A virus A/chicken/Tarutung/BPPVI/2...
I. A virus (A/chicken/Deli A/chicken/... I. A virus (A/chicken/Deli A/chicken/...
I. A virus A/Indonesia/CDC625L/2006 NP I. A virus A/chicken/Dairi/BPPVI/2005 NA
I. A virus A/Indonesia/CDC599/2006 NP I. A virus A/chicken/Simalanggang/BPP...
I. A virus A/Indonesia/CDC625/2006 NP I. A virus A/Indonesia/CDC595/2006 NA
I. A virus A/Indonesia/CDC599N/2006 NP I. A virus A/Indonesia/CDC596/2006 NA
I. A virus A/Indonesia/CDC594/2006 NP I. A virus A/Indonesia/CDC597/2006 NA
I. A virus A/Indonesia/CDC595/2006 NP I. A virus A/Indonesia/CDC599/2006 NA
I. A virus A/Indonesia/CDC597/2006 NP I. A virus A/Indonesia/CDC594/2006 NA
I. A virus A/Indonesia/CDC596/2006 NP I. A virus A/Indonesia/CDC625/2006 NA
I. A virus A/Indonesia/CDC625L/2006 NA
0.002

0.002
GENETICAL HOMOLOGY OF H1N1
INFLUENZA A VIRUS
GENETICALY OF H1N1 INFLUENZA A
VIRUS
GENETICALY OF H1N1
INFLUENZA A VIRUS
 PHYLOGENETIC OF ND ?

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Oct. 2008
PHYLOGENETIC ANALYSIS OF ND BASE ON NUCLEOTIDA IN THE
 HOME WORK

1. What kinds of virus components and their

function of transcription, translation and
multiplication became infectious particle ?
2. What kinds of strategy of protein synthetic
that are inducing of single gen ?
THANK YOU

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