Beruflich Dokumente
Kultur Dokumente
Kat Pierce
ID Number:
1461881
Section:
BX
Lab Partner: John Redfield
Chem 142 Experiment #3: Calibration Curves and an Application of Beer's Law
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report, and that all calculations and responses other than the reporting of raw data are your own independent
work. Failure to sign this declaration will result in 5 points being deducted from your report score.
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Note:
All sections of
this report must
be typed
470 nm
2 pt
Ferroin:
Concentration (M)
1.25 x 10^-5
2.50 x 10^-5
3.75 x 10^-5
5.00 x 10^-5
6.25 x 10^-5
Absorbance
0.120
0.186
0.320
0.432
0.560
Absorbance
vs.your
Concentration
Place your calibration
plot here. Make
plot big enough to cover this
instruction
box
so
that
it
is
large
enought
for someone else to read.
0.6
y = 9008x - 0.0142
R = 0.9903
This calibration plot is Abs vs. concentration of ferroin (M) (y-axis vs. xaxis)0.5
4 pts
Absorbance
Use the
0.4 online resources if you need help figuring out how to plot a
graph in Excel.
0.3
Title the graph and label the axis, including the correct units
(Absorbance data is unitless). Be sure to double check your units and
0.2
formatting
once you print the report.
0.1
Add a Trendline to show the linear fit of your data. Choose a linear line
andchoose the options that will "display the equation on the chart",
0
including the R2 value.
0
0.00001
0.00002
0.00003
0.00004
0.00005
0.00006
0.00007
2 pts
436
mg
2)
20
mg
3)
100
mL
4)
mL
5)
mL
6)
7)
100
0.051
Using the calibration equation and the absorbance you measured for the prepared sample,
calculate the ferroin concentration. Show your work and don't forget to include units.
8)
3 pts
Based on the procedural steps and the ferroin concentration you just calculated, calculate the
moles of ferroin in the final ferroin complex solution prepared in Part II, Step 9. Show your work,
including units.
mol = M X V
M of ferroin: 7.24x10^-6 M
V = 100 mL of ferroin complex solution x 10^-3 = 0.1 L
7.24x10^-6 M x 0.1 L = 7.24x10^-7 moles of ferroin
9)
3 pts
Based on the moles of ferroin in the final ferroin complex solution, calculate the moles of iron in
the crushed tablet solution prepared in Part II, Steps 2-5. Show your work, including units.
ferroin ion: Fe2+ in complex = 1:1 ratio
7.24x10^-7 moles of ferroin x 20.0 dilution factor = 1.45x10^-5 moles of iron in the crushed tablet
soln
10)
Using the "moles of iron in the crushed tablet solution" you just calculated, calculate the mass (in
mg) of iron in the crushed sample that you weighed out. Show your work, including units.
1.45x10^-5 moles of iron x (55.845 g Fe/1 mol) = 8.087x10^-4 g of Fe
8.087x10^-4 g of iron x (1000 mg/1 g) = 0.809 mg of Fe
11)
3 pts
From the mass of iron in the crushed tablet sample you weighed out, calculate the mass (in mg)
of iron in a whole tablet. Show your work, including units.
0.809 mg of Fe crushed tablet x (436 mg average mass of tablet)/27 mg of iron per tablet on
bottle= 13 mg of iron in whole tablet
12)
3 pts
27
mg
3 pts
2. If you did not wait for the complete formation of the ferroin complex in Part II, step 10, how would your Abs data be different?
Explain how would this affect your determination of the mass of iron in the tablet? (4 pts)
If you do not wait for the ferroin complex formation to complete, then the absorbance will be less. If the process of the formation
does not finish, then the concentration of ferroin will not be accurate. Without an accurate concentration, you cannot calculate an
accurate absorbance. You cannot determine the mass of iron in the tablet without knowing the ferroin concentration or absorbance.
3. You use atomic emission spectroscopy, another spectroscopic technique, to measure the Li+ concentration in 5 standard
solutions of varying concentrations of LiCl. The intensities for the standard solutions are plotted versus the concentrations and the
resulting calibration equation is:
Intensity = 83,100 M-1 * [Li+] + 1.65
If the intensity of your unknown sample is 101, what is the concentration of Li+ in the analyzed sample? (4 pts)
Intensity = 83,100 M^-1 * [Li^+] + 1.65
101 = 83,100 M^-1 * [Li^+] + 1.65
99.35 = 83,100 M^-1 * [Li^+]
99.35/ 83,100 M^-1 = 0.001195 M
[Li+] = 0.001195 M
If 15 mL of the original unknown sample was diluted to 350. mL prior to analysis, what is the concentration of Li+ in the original
solution? (4 pts)
0.001195 M x (0.35 L/0.015 L) = 0.028 M [Li+]
Concentration
0.0000125
(M) (x 10-5)
0.000025 0.0000375
Absorbance
0.12
0.186
0.32
0.00005 0.0000625
0.432
0.56
y = 9008x
R = 0.9903
Absorbance
0.5
0.4
0.3
0.2
0.1
0
0
s. Concentration
y = 9008x - 0.0142
R = 0.9903