Perrin Kileen

Crime Scene 1
Biology
Nucleic DNA Analysis:
In the winter of 1868 a young Swiss doctor named Friedrich Miescher, operating
within the laboratory of Felix Hoppe-Seyler at the University of Tbingen, performed
experiments on the chemical composition of leukocytes that result in the invention of
deoxyribonucleic acid. In his experiments, Miescher detected a precipitate of associate
unknown substance, that he characterized any. Its properties throughout the isolation
procedure and its resistance to proteolytic enzyme digestion indicated that the novel
substance wasn't a macromolecule macromolecule. Analyses of its elementary
composition discovered that, in contrast to proteins, it contained giant amounts of
phosphoric and, as Miescher confirmed later, lacked sulphur. Miescher recognised that
he had discovered a completely unique molecule. Since he had isolated it from the cells'
nuclei he named it nuclein, a reputation preserved in today's designation
deoxyribonucleic acid. In ulterior work Miescher showed that nuclein was a
characteristic part of all nuclei and hypothesised that it'd sway be inextricably coupled to
the operate of this organ. He steered that its abundance in tissues may well be
associated with their physiological standing with will increase in "nuclear substances"
preceding biological process. Miescher even speculated that it would have a task within
the transmission of hereditary traits, however afterwards rejected the concept.this text
reviews the events and circumstances resulting in Miescher's discovery of
deoxyribonucleic acid and places them inside their historic context. It additionally tries to
elucidate why it absolutely was Miescher World Health Organization discovered
deoxyribonucleic acid and why his name isn't universally related to this molecule these
days.
Several basic steps are done during DNA testing which doesnt matter on the
type of test being done. This procedure includes: 1. the isolation of the DNA from an
evidence sample containing DNA of unknown origin, and usually at a later time, the
isolation of DNA from a sample from a known individual; 2. the processing of the DNA
so that test results may be obtained; 3. the determination of the DNA test results, from
specific regions of the DNA; and 4. the comparison and reading of the test results from
the unknown and known samples to determine whether the known individual is not the
source of the DNA or is included as a possible source of the DNA.
Laboratory techniques supported nucleic acid methods have increased in quality
over the last decade with clinical microbiologists. This increase in quality may be a
result of advances created in nucleic acid amplification and detection techniques.
polymerase chain reaction, the first nucleic acid amplification technique, modified the
means many people viewed and used nucleic acid techniques. Then the potential of

polymerase chain reaction became apparent, other ways of nucleic acid amplification
and detection were developed.
Based on the DNA around the victims step son we were able to conclude that
Michell Lee, the victims wife, was involved in the murder of Aaron Lee. With this
information we were able to determine her as the shooter for her blood spatter was on a
piece of evidence.

http://www.ncbi.nlm.nih.gov/pubmed/17901982