Beruflich Dokumente
Kultur Dokumente
email: lmasterson01@hamline.edu
EDUCATION
Ph.D., Chemistry (emphasis: Chemical Biology, December 2008)
University Of Minnesota, Minneapolis, MN
B.Sc., Chemistry with American Chemical Society Certification (May 2003)
University of Wisconsin- La Crosse, La Crosse, WI
PROFESSIONAL SERVICE
2014-present, Editorial Board for Medical Research Archives
2014 (July) Poster Judge for the 28th Annual Symposium for the Protein Society
2014 (June) Review Committee for International Virtual Scientific Conference
2013-present, Advisory Board for Neo-Cardial Technologies, LLC
2013-present, Ad Hoc Organizer and Founding Member of the ACTC Biophysics Club
2007
2006
Curriculum Vitae
2006
2005
2005
2005
2003
2002
2001
2000, 2001
PEER-REVIEWED PUBLICATIONS
(Undergraduates denoted with an asterisk)
1) J. Kim, L.R. Masterson, A. Cembran, R. Verardi, L. Shi, J. Gao, S.S. Taylor, and G. Veglia.
Dysfunctional Conformational Dynamics of Protein Kinase A Induced by a Lethal Mutant of
Phospholamban Hinder Phosphorylation (2015, in press) Proc. Natl. Acad. Sci.
2) A.K. Srivastava, A. Cembran, J. Kim, L.R. Masterson, and G. Veglia. Synchronous Opening
and Closing Motions are Essential for cAMP-Dependent Protein Kinase A Signaling (2014)
22 (12), 1735-1743, Structure.
3) F.A. Chao, A. Morelli, J.C. Haugner, L. Churchfield, L.N. Hagmann, L. Shi, L.R. Masterson,
R. Sarangi, G. Veglia, and B. Seelig. Structure and dynamics of a primordial catalytic fold
generated by in vitro evolution (2013) 81-83, Nat. Chem Bio.
4) A. Cembran, L.R. Masterson, C.L. McClendon, S.S. Taylor, J. Gao, G. Veglia.
Conformational equilibrium of N-myristoylated cAMP-dependent protein kinase A by
molecular dynamics simulations (2012) 51, 10186-10196, Biochemistry.
5) N.J. Traaseth, F.A. Chao, L.R. Masterson, S. Mangia, M. Garwood, S. Michaeli, B. Seelig,
and G. Veglia. "Heteronuclear Adiabatic Relaxation Dispersion (HARD) for quantitative
analysis of conformational dynamics in proteins" (2012) 219, 75-82, J. Magn. Reson.
6) F.A. Chao, L. Shi, L.R. Masterson, and G. Veglia. "FLAMEnGO: a fuzzy logic approach for
methyl group assignment using NOESY and paramagnetic relaxation enhancement data"
(2012) 214 (1), 103-110, J. Magn. Reson.
7) L.R. Masterson, T. Yu, L. Shi, Y. Wang*, M. Gustavsson, M.M. Mueller*, and G. Veglia.
"cAMP-dependent protein kinase A selects the excited state of the membrane substrate
phospholamban" (2011) 412 (4), 155-164, J. Mol. Biol.
8) L.R. Masterson*, E.C. Gaffarogullari**, E.E. Metcalfe, N.J. Traaseth, E. Balatri, M.M. Musa,
D. Mullen, M.D. Distefano, and G. Veglia. "A myristoyl/phosphoserine switch controls
cAMP-dependent protein kinase A association to membranes" (2011) 411 (4) 823-836, J.
Mol. Biol. (**equal contributions).
9) Porceilli, C. Olivieri, L.R. Masterson, Y. Wang*, and G. Veglia. "Templating -amylase
peptide inhibitors with organotin compounds" (2011) 16 (8), 1197-1204, J. Biol. Inorg.
Chem.
Larry R. Masterson, Ph.D.
Curriculum Vitae
10) L.R. Masterson, L. Shi, E.E. Metcalfe, J. Gao, S.S. Taylor, and G. Veglia. "Dynamically
committed, uncommitted, and quenched states encoded in protein kinase A revealed by
NMR spectroscopy " (2011) 108 (17), 6969-6974, Proc. Natl. Acad. Sci.
11) K.H. Ha, L.R. Masterson, Z. Hou, R. Verardi, N. Walsh*, G. Veglia, and S.L. Robia. "Lethal
Arg9Cys phospholamban mutation hinders Ca2+-ATPase regulation and phosphorylation by
protein kinase A" (2011) 108 (7) 2735-2740, Proc. Natl. Acad. Sci.
12) L. R. Masterson, C.Y. Cheng, T. Yu, M. Tonelli, A. Kornev, S. S. Taylor, and G. Veglia.
"Dynamics Connect Substrate Recognition to Catalysis in Protein Kinase A" (2010) 6 (11),
821-828, Nature Chemical Biology.
13) M. Tonelli, L.R. Masterson, G. Cornilescu, J.L Markley, and G. Veglia. One-Sample
Approach to Determine the Relative Orientations of Proteins in Ternary and Binary
Complexes from Residual Dipolar Coupling Measurements (2009) 131 (40), 14138-14139,
JACS.
14) L.R. Masterson, L. Shi, M. Tonelli, A. Mascioni, M.M. Mueller*, and G. Veglia. Backbone
NMR resonance assignment of the catalytic subunit of cAMP-dependent protein kinase A in
complex with AMP-PNP (2009) 3, 115-117, Biomolecular NMR Assignments.
15) F. Porcelli, D. Triggiani, B.A. Buck-Koehntop, L.R. Masterson, and G. Veglia.
Pseudoenzymatic dealkylation of alkyltins by biological dithiols (2009) 14, 1219-1225, J.
Biol. Inorg. Chem.
16) L.R. Masterson, N. Bortone, T. Yu, K.N. Ha, E.C. Gaffarogullari, O. Nguyen*, and G. Veglia.
Expression and purification of isotopically labeled peptide inhibitors and substrates of
cAMP-dependant protein kinase A for NMR analysis (2009) 64, 231-236, Protein
Expression and Purification.
17) L.A. Behling*, S.C. Hartsel, D.E. Lewis, A.A. DiSpirito, D.W. Choi, L.R. Masterson, G.
Veglia, W.H. NMR, mass spectroscopy and chemical evidence reveal a different chemical
structure for methanobactin that contains oxazolone rings (2008), 130, 12604-12605,
JACS.
18) L.R. Masterson, M. Tonelli, J.L. Markley, and G. Veglia. Simultaneous detection and
deconvolution of congested NMR spectra containing three isotopically labeled species
(2008) 130, 7818-7819, JACS.
19) N.J. Traaseth, K. Ha, R. Verardi, L. Shi, J.J. Buffy, L.R. Masterson, and G. Veglia.
Structural and Dynamic Basis of Phospholamban and Sarcolipin Inhibition of Ca2+-ATPase
(SERCA) (2008), 47, 3-13, Biochemistry.
20) L.R. Masterson, A. Mascioni, N.J. Traaseth, S.S Taylor and G. Veglia. Allosteric
Cooperativity in Protein Kinase A (2008), 105, 506-511, Proc. Natl. Acad. Sci.
21) M. Tonelli, L.R. Masterson, K. Hallenga, G. Veglia, and J. L. Markley. Carbonyl Carbon
Label Selective (CCLS) 1H-15N HSQC Experiment for Improved Detection of Backbone 13C15
N Cross Peaks in Larger Proteins (2007), 29 (3), 177-185, J. Biomol. NMR.
Larry R. Masterson, Ph.D.
Curriculum Vitae
22) L.R. Masterson, M. Etienne, F. Porcelli, G. Barany, R.P. Hammer, and G. Veglia. NonStereogenic -Aminoisobutryl-Glycyl Dipeptidyl Unit Nucleates Type I -Turn in Linear
Peptides in Aqueous solution (2007), 88 (5), 746-753, Biopolymers.
23) L.R. Masterson, M.A. Etienne, G. Barany, R.P. Hammer, and G. Veglia, Impact of a Single
-Disubstituted Amino Acid on -Hairpin Folding (2006) Proceedings of the 19th
American Peptide Symposium, 314.
24) S. Gazal, L.R. Masterson, and G. Barany, Facile Solid-Phase Synthesis of C-terminal
Peptide Aldehydes and Hydroxamates from a Common Backbone Amide Linked (BAL)
Intermediate (2005), 66 (6), J. Pep. Res., 324-332.
25) L.R. Masterson. Unlocking the Structure and Flexibility of a Four Residue Peptide Using 1H
NMR Spectroscopy (2002) UW-La Crosse Journal of Undergraduate Research. V, 381389.
BOOK CHAPTERS
1) R. Verardi, N.J. Traaseth, L.R. Masterson, V.V. Vostrikov, G. Veglia. Isotope labeling for
solution and solid-state NMR spectroscopy of membrane proteins (2012) 992, 35-62. Adv.
Exp. Med. Biol.
2) L.R. Masterson, A. Cembran, L. Shi, and G. Veglia. "Allostery and binding cooperativity of
the catalytic subunit of protein kinase A by NMR spectroscopy and molecular dynamics
simulations" (2012) 87, 363-389, Adv. Protein Chem. Struct. Biol.
ORAL PRESENTATIONS
1) Molecular details of abnormal heart function. Hamline University, St. Paul, MN, September,
2014.
2) Defining the molecular details of abnormal heart function. University of Wisconsin- La
Crosse, La Crosse, WI, May, 2014.
3) Defining the molecular details of abnormal heart function. University of St. Thomas, St. Paul,
MN, March, 2014.
4) Defining the molecular details of abnormal heart function. University of Wisconsin-Eau
Claire, Eau Claire, WI, September, 2013.
5) Structural dynamics of protein kinase A bound to substrate and inhibitor. L.R. Masterson
and G. Veglia. The 14th Bollum Symposium, University of Minnesota, Minneapolis, MN, May,
2012.
6) Towards the full molecular details of protein kinase A mediated catalysis by NMR
spectroscopy. Hamline University, St. Paul, MN, March, 2012.
Larry R. Masterson, Ph.D.
Curriculum Vitae
7) Towards the full molecular details of protein kinase A mediated catalysis by NMR
spectroscopy. Boyer-Peter Award Presentation, Minneapolis, MN, May, 2011.
8) Biophysics holds the keys to the future of technology. Minneapolis Rotary Club,
Minneapolis, MN, March, 2011.
9) Towards the full molecular details of protein kinase A mediated catalysis by NMR
spectroscopy. University of Minnesota, Minneapolis, MN, November, 2008.
10) Educational experiences: From a Small City University to a Large Metropolitan University.
Masterson, LR. University of Wisconsin-Eau Clare, Eau Clare, WI, November, 2008.
11) Probing Protein Kinase A Cooperativity, Allostery, and How Phosphorylation Kinetics
Correlate with Dilated Cardiomyopathy. Biochemistry, Molecular Biology, and Biophysics
Fall 2008 Retreat, Alexandria MN, October, 2008.
12) Aberrations in the Mechanism of Protein Kinase A Mediated Phosphorylation Caused by
Phospholamban Mutants. Masterson, LR, Taylor, SS, Veglia, G. Biophysical Society 52 nd
Annual Meeting, Long Beach, CA, February, 2008.
13) Intramolecular Signaling in Protein Kinase A as Probed by NMR. Masterson LR, Veglia, G.
University of Minnesota 6th Annual Graduate Student Symposium, Department of Chemistry,
Minneapolis, MN, May, 2007.
14) Unlocking the Structure and Flexibility of a Four Residue Peptide Using 1H NMR
Spectroscopy. Masterson, LR, Loh, A. Ruhr Universitt Bochum Ph.D. Candidate Seminar,
Bochum, Germany, August, 2003.
15) Unlocking the Structure and Flexibility of a Four Residue Peptide Using 1H NMR
Spectroscopy. Masterson, LR, Loh, A. University of Wisconsin-La Crosse Department of
Chemistry Undergraduate Seminar, May, 2002.
POSTER PRESENTATIONS
(Undergraduates denoted with an asterisk)
1) Recognition and conversion of flunitrazepam by Cyp3A4 is altered by caffeine. M. Volker*,
T. Larson*, L.E. Sparks*, and L.R. Masterson. Biophysical Society Regional Networking
Event, La Crosse, WI, October, 2014.
2) Spectroscopic investigation of the structural perturbation of tau microtubule binding domains
at the golgi membrane as illustrated by membrane mimics. L. Sparks* and L.R. Masterson.
Biophysical Society Regional Networking Event, La Crosse, WI, October, 2014.
3) Thermodynamic analysis of membrane interactions with tau peptides. R. Usgaard*, M.
Culp*, L.E. Sparks*, and L.R. Masterson. Biophysical Society Regional Networking Event,
La Crosse, WI, October, 2014.
Curriculum Vitae
Curriculum Vitae
17) Structural dynamics of protein kinase A bound to substrate and inhibitor. L.R. Masterson
and G. Veglia. The 14th Bollum Symposium, University of Minnesota, Minneapolis, MN, May,
2012.
18) Automated assignment of methyl-TROSY spectra using sparse NOESY and PRE data. F.
Chao, L.R. Masterson, L. Shi, and G. Veglia. Experimental Nuclear Magnetic Resonance
Conference, Pacific Grove, California, March, 2011.
19) The role of N-myistoylation of cAMP-dependent protein kinase A in recognition and
phosphorylation of membrane bound substrates. E.C. Gaffarogullari, L.R. Masterson, N.J.
Traaseth, D. Mullen, M.M. Musa, E. Balarti, M.D. Distefano, and G. Veglia. 55th Annual
Biophysical Society Meeting, Baltimore, MD, 2011.
20) NMR reveals backbone dynamics encode functional and inhibitory states in protein kinase
A. L.R. Masterson and G. Veglia. Experimental Nuclear Magnetic Resonance Conference,
Daytona Beach, Florida, April, 2010.
21) Molecular Details of the Interaction of Protein Kinase A with Phospholamban and
Phospholamban Mutants. L.R. Masterson, T. Yu, L. Shi, and G. Veglia. Protein Society
Annual Meeting, Boston, Massachusetts, July, 2009.
22) One-Sample Approach for the Structural Determination of Protein-Protein Complexes by
NMR Spectroscopy. L.R. Masterson, T. Yu, L. Shi, and G. Veglia. Experimental Nuclear
Magnetic Resonance Conference, Pacific Grove, California, March, 2009.
23) Structural and Dynamic NMR Characterization of the Endogenous Inhibitor PKI Free and
Bound to Protein Kinase A. F. Porcelli, L.R. Masterson, T. Yu, S.S. Taylor, L. Shi, and G.
Veglia. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, California,
March, 2009.
24) NMR Spectroscopic and Kinetic Investigations of the Interaction of Protein Kinase A with
Phospholamban and Phospholamban Mutants. L.R. Masterson, T. Yu, L. Shi, and G.
Veglia. Institute for Complex Adaptive Matter (ICAM) Meeting, San Diego, California,
December, 2008.
25) Simultaneous Detection and Deconvolution of Congested Spectra Containing Isotopically
Labeled Enzyme, Substrate, and Inhibitor. L.R. Masterson, M. Tonelli, J.L. Markley, and G.
Veglia. Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, California,
March, 2008.
26) Abberations in the Mechanism of Protein Kinase A Mediated Phosphorylation Caused by
Phospholamban Mutants. L.R. Masterson and G. Veglia. 52nd Annual Biophysical Society
Meeting, Long Beach, California, February 2008.
27) Structural Basis for Dilated Cardiomyopathy. L.R. Masterson, S.S. Taylor, and G. Veglia.
Keystone Symposia (Frontiers in Structural Biology), Steamboat, Colorado, January, 2008.
28) Intramolecular Signaling in Protein Kinase A as Probed by NMR. L.R. Masterson, S.S.
Taylor, and G. Veglia. Chemical Biology Interface Training Grant Summit, Bethesda,
Maryland, June, 2007.
Larry R. Masterson, Ph.D.
Curriculum Vitae
29) Intramolecular Signaling in Protein Kinase A as Probed by NMR. L.R. Masterson, S.S.
Taylor, and G. Veglia. Biophysical Society 51st Annual Meeting, Baltimore, Maryland,
March, 2007.
30) Coupled Motions in Protein Kinase A as Characterized by NMR. L.R. Masterson, S.S.
Taylor, and G. Veglia. Keystone Symposia (Frontiers in Structural Biology), Salt Lake City,
Utah, January, 2007.
31) Coupled Motions in Protein Kinase A as Characterized by NMR. L.R. Masterson, N.J.
Traaseth, A. Mascioni, S.S. Taylor, and G. Veglia. Experimental Nuclear Magnetic
Resonance Conference, Pacific Grove, California, April, 2006.
32) Defining Allosteric Regulation in Protein Kinase A. L.R. Masterson, A. Mascioni, N.J.
Traaseth, S.S. Taylor, and G. Veglia. Keystone Symposia (Frontiers in Structural Biology),
Keystone, Colorado, January, 2006.
33) Impact of a Single -Disubstituted Amino Acid on -Hairpin Folding. L.R. Masterson, M.A.
Etienne, G. Barany, R.P. Hammer, and G. Veglia. 19th American Peptide Symposium, San
Diego, California, June, 2006.
34) Studying the Structure and Flexibility of a Short 310 helical Peptide using 1H NMR
Spectroscopy. L.R. Masterson and A. Loh. UW-L Celebration of Undergraduate Research
and Creativity, La Crosse, Wisconsin, April, 2002.
35) Unlocking the Structure and Flexibility in a Series of Peptides Using 1H NMR Spectroscopy.
L.R. Masterson and A. Loh. National Conference of Undergraduate Research- Whitewater,
Wisconsin, May, 2002.
36) Unlocking the Structure and Flexibility in a Series of Peptides Using 1H NMR Spectroscopy.
L.R. Masterson and A. Loh. Biophysical Society 46th Annual Meeting, San Francisco,
California, April, 2002.
37) Studying the Structure and Flexibility of a Short 310 helical Peptide using 1H NMR
Spectroscopy. L.R. Masterson and A. Loh. UW-L Celebration of Undergraduate Research
and Creativity, La Crosse, Wisconsin, April, 2001.
Curriculum Vitae
Curriculum Vitae
UW-L Undergraduate Research Grant, Unlocking the Structure and Flexibility of a Series of
Short Peptides Using 1H NMR Spectroscopy (8/2001-6/2002)
Goals: Solution state peptide synthesis of short 310-helical peptides and use NMR to
characterize the thermodynamic stability of their secondary structure.
Role: Student Researcher
10
Curriculum