Biotechnological approach of converting lingo cellulosic biomass is the most

important need in the industry. As the non- renewable energy sources are in the
danger of depletion alternative but economically cheap source should be replaced.
The lingo cellulosic mass is found to be an abundant as well as inexpensive resource
of renewable energy. Microbial cellulases are playing multiple roles in biomass
conversion into fuel, waste treatment and other industries also. Bacteria and fungus
are useful in production of cellulase enzymes commercially. The biological
degradation of cellulose has been studied for many years, and a number of
cellulolytic enzymes, especially cellulases produced by fungi and bacteria, have
been isolated and characterized [32]. These enzymes, which cleave the -1,4 bond
of cellulose, belong to the large family of glycosyl hydrolases. On the basis of
sequence comparisons and hydrophobic cluster analysis, the catalytic domains of all
glycosyl hydrolases have been classified into 63 families of homologous folds [8, 9,
10].When compared to bacterial cellulases, fungal cellulases are simple. Due to its
various industrial application many scientist are attracted towards cellulase and its
application. There are more than 57,000 entries in NCBI for cellulase enzymes.
Among this 39632 organisms belong to bacteria are also recorded as the cellulase
producers. In these proteo bacteria and actino bacteria are the highest producers of
these enzyme modules.
In this proposed study the cellulose degrading organism was isolated from the
tamarind kernel powder and is showing significant production of the enzyme. The
sequence analysis of 16sRNA results revealed the identity of the organism. They are
found to belong to the Pseudomonas, proteo bacteria in its nature. The phylogenetic
analysis is also explain the relationship between the cellulose degrading organism.
This study will provide more knowledge on the cellulose degrading organism and its
evolution.