A.1.

13
AOAC Official Method 942.27
Standard Solutions
of Sodium Thiosulfate
First Action 1942
Final Action
A. Preparation of Standard Solution

Dissolve ca 25 g Na2S2O3@5H2O in 1 L H2O. Boil gently 5 min and

transfer while hot to storage bottle previously cleaned with hot
chromic acid cleaning solution and rinsed with warm boiled water.
(Temper bottle, if not heat-resistant, before adding hot solution.)
Store solution in dark, cool place; do not return unused portions to
stock bottle. If solutions less concentrated than 0.1M are desired,
prepare by dilution with boiled water. (More dilute solutions are less
stable and should be prepared just before use.)
B. Standardization

Accurately weigh 0.200.23 g K2Cr2O7 (NIST SRM 136e dried

2 h at 100C) and place in glass-stoppered iodine flask (or
glass-stoppered flask). Dissolve in 80 mL chlorine-free water
containing 2 g KI. Add, with swirling, 20 mL ca 1M HCl and
immediately place in dark 10 min. Titrate with Na2S2O3 solution, A,
adding starch solution after most of I2 has been consumed.
Molarity (mol/L) = g K2Cr2O7 1000/mL Na2S2O3 49.032
References: JAOAC 25, 659(1942); 27, 557(1944); 28,
594(1945); 38, 382(1955); 47, 43, 46(1964); 48,
103(1965).

2005 AOAC INTERNATIONAL