Name. Anse Key Exam IIL November 3, 2014 100 pts 1.(10 pts). Last Friday during the review, what two example chemical analysis problems did I discuss, You need to have specific details for sre here. vrcussecl the dircleon!) thensue - pee ein lta Spe >» oe Dereey a ae x 2 Pucliasha noslinnc wh Por aod aud sd, Auays !) leu b heer éf lewabedf Clr. Pats aceosnewed, 2.(Spts). __In an atomic absorption experiment, researchers found that a strong transition was detected at 425 nm, What energy does this correspond , in kJ/mol. Fa be 2 Cosne™sx) oer) 108220" Sedan 425% 10 Tae (docx TH) (e0enso Rpfetiul |) — 22a 5y = ZB) fide 3.(Spts). In. molecular absorption experiment, a student measured a transmittance of 48%. ‘Assuming good background correction and correction for all other non-absorbance light loss mechanisms, what absorbance does this transmittance correspond to? As -loyT = - bos G42) = 6,32 Compare and contrast the performance characteristics of a photodiode, jer and photodiode array detector. Plctusde — tung abl litres, Plt eluiren Peeducecl at pleticattude Peepectinal atch 5) daslet Pr nuddybaton pv auplfsaton . Avigl. haul dtetr Ie" (pls. Wp aay, Lev Ceplit escticache, Aitzatid Pom — dablehauel detiote, ulus visas abtgh 1, odtcitdd deibtisienly « Sid ausogtty Jordi Ce ceeviase Shs Guabivonustily Jo Luts Hadas pom5.(S pts). In an atomic absorption measurement using a flame, name three processes that occur fo get the sample from an ion in a condensed phase (water) to neutral atoms in the gas phase. 1. Ne bulsaten —> prusiteon 5) genall pole aerosol potiles 2. Desetoaten —s It eowrnicat 9) flow caus gelowobevag, 3. Abrayatin —> fet Cxrvesnunit lase praclesss erlorar € Gea phase - Ve /forre Fin Yetuah ee The following questions pertain to this chemical analysis problem. - CHO. / - + nay + ov —e OIE ‘CHO NDA Naphthalene dialdehyde is a derivatizing agent that can react with primary amines (amino acids) to yield the product on the right. You are a bioanalytical chemist working on developing the most sensitive assay for amino acids with the lowest limits of detection. NDA has a strong absorption maximum at 456 nm, which is very near the 442 nm line of a He-Cad laser and well within the output wavelength range of a tungsten lamp. 6(10 pts). Sketch a detailed drawing of the instrument you would use for this measurement. Specifies need to be given on the detector selection and why, and the wavelength selector. Soguuatin ailed Ct Hee git devidese cstly curl Lewat £00. Pecsenseeuse itistirs wert be tuzesin Uo chmebects cdi Luze~ciahuted) Jlsosecuse ceed & Hehe : FidLbald) (sy we | ber Shalt. - aN tei Poe Ey ailleurs yr TB Grvect Ge source Pine Comeit dewratria dLotzele, Cath, Clacantam 2 thau P0AD Rewuber TemR seany chase Liv QR (Frente taacitiom Cau preduge a Fluchuehiy oudbpibseg7.(5 pts). Give an analytical expression that relates the detector signal to the analyte concentration. Explain all terms, ee C= anadts cone Fa. = ee eek T ts buat posession le €2 miner Coefeainck a ac. ttn 0 Pleoerers b= path leuk, & ae a tial ~ h oleewlen ett 8. (5 pts). Linear regression analysis of the NDA-amino acid complex produced the following equation, y = 0.065 (abs signal/M)x + 0.002, Measurement of an unknown produced an absorbance value of 0.365. What is the concentration of the unknown? Yemurb = 0.06054 +6.06% 6365s O00T% +O S82 x: O.3wS-0.00% Gia) oe “ 0.065 aE) Slee Sibu 9. (10 pts). Deseribe (show an energy level diagram) what is happening photophysically to the molecule in the measurement you propose. Give specific energy for the excitation, The following questions pertain to the following chemical problem. ‘The analysis of wastewater for trace metal contamination is an important step in ensuring human and environmental health. Wastewater is regulated differently in different countries, but the goal is to minimize the pollution introduced into natural waterways. Two automobile industry wastewater samples (treated and untreated) and one tap water sample were collected and preserved for the analysis of Cd"?, Pb", Ni? and Cu’,10. (10 pts). Sketch a detailed drawing of the instrument you would use for this measurement. ‘You need to provide some justification for the selection. He ain G, Ther ove. th tently crs) Free Aasegua: 1) Boaz cleauge Da Flac ARS on a gry bel tole fecccres ARS. . aate dereces CHOL's) eee fe Cachebecicit Sona 4 tase, Meer. cgttevgatern of uccdocreseaelt te sci edu fox Cok, ebouwit- ~ Cuglats tube flouade old d An arabfedrigle aud Ble prrcale Looe LOD: dak quate tof ft bea toresatent BB ate 2) Baa Aug) au TPES @ TEP PS — puiltecdousitt. qualypes capably eth rut At Remedetene ~ bat ddicbain fijene! Dyscsett weit Glaly Le baud werd the pei 11.(S pts). What are three adVantages of ICP over AAS or AES for elemental analysis? ee _ Sey ee 1. Muck hatte Cuvrninnacl So mock sucker buchen afiisie, vase 2. Chauiatly eusit ~0 fewer chest tiliifesesg —Ar gaa! 3. dw dell) Qudiiny Leadils mulk-elewut auabed 12.(5 pts), An internal standard method was used for quantifying Pb’? (207.2g/mol). The we signal for the unknown was 0.021. The signal for the internal standard was 0.151. The response factor, F, was 5. What is the unknown Pb” concentration in ppm? ious a Als} @.021) (jon Ae e (As Ce = Abs _ Coefinrt ise ‘4) Cal ts F . (5) (51 = ABO” eet (Pee = S80 '"g poms mah. ppt=ra] sec t= oer 3 peb> pale = 58x ppm 13. (6 pts). In this analysis, some of the Pb"? was not free and dissolved in solution but bound ‘as PbO2. Your task is to measure total Pb’? content. How might you solve this problem? Geel thin tout wock. welh dove howdy, Gin eamttl Airole abl nal eves Speess geo aus auatyn 17 Ve tel 9 tons [ebeuitth Rewuuler oll anal, eu Han case Pb , aucdds % Ge lvl! vi setukm ao PL. 414, (5 pls). During the assay development, it was decided that comparing the performance of AAS and ICP would be in order. Why was the dynamic range larger and the limit of detection significantly lower for ICP? 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