Bioinformatics Preliminary Lab

1. What does Amylase do?

Hint:
Give findings from Swissprot, Genecards and KEGG
Include screen snapshots of your findings
2. Obtain the sequences of two (2) yeast amylase proteins.
Hint:
Use Swissprot at expasy
Include screen snapshots of your findings
3. Give the ENA accession number of AMY2B_HUMAN.
Hint:
Use EBI ENA
Include screen snapshots of your findings
4. Obtain the AMY2B_HUMAN gene.
Hint:
Use EMBL, ACcession number from ENA
Include screen snapshots of your findings
5. Give the partial genomic DNA of a mouse and translate it into protein.
Compare to partial human protein amylase 2B.
Hint:
EBI: dbfetch
Include screen snapshots of your findings
6. List two products of Human Recombinant Protein and antibody for AMY2B respectively.
Hint:
Use Genecards.
Include screen snapshots of your findings
7. Predict the protein secondary structures of human and mouse amylase
Hint:
Expasy: GOR
Include screen snapshots of your findings
Relevant webpages:
1. Swissprot at expasy
2. NCBI (ORF Finder)
3. EBI
4. Genecards at DKFZ
5. KEGG

Send your answers in pdf format to eh222.cbe647@gmail.com by Sunday midnight 11.59 pm

21 September 2014. Write your full name and student number (eg. Johnny Depp_2011xxxxxx)
as the subject of your email.