Tiempo (h)

Concentracion de
celulas, x (kgm-3)

Concentracion de
sustrato (kgm-3)

0
0.33
0.5
0.75
1
1.5
2
2.5
2.8
3
3.1
3.2
3.5
3.7

0.2
0.21
0.22
0.32
0.47
1
2.1
4.42
6.9
9.4
10.9
11.6
11.7
11.6

25
24.8
24.8
24.6
24.3
23.3
20.7
15.7
10.2
5.2
1.65
0.2
0
0

30
Concentracin
25
20
15
10

5
Siempre debo
empezar0de 0 p
los cultivos
0

Ysx global
Tiempo (h)

Ln X (kgm-3)

U (1/h)
Ln X

0
0.33
0.5
0.75
1
1.5
2
2.5
2.8
3
3.1
3.2
3.5
3.7

-1.60943791
-1.56064775
-1.51412773
-1.13943428
-0.75502258
0.00000000
0.74193734
1.48613970
1.93152141
2.24070969
2.38876279
2.45100510
2.45958884
2.45100510
Umax

0.15
0.27
1.50
1.54
1.51
1.48
1.49
1.48
1.55
1.48
0.62
0.03
-0.04

3.00
2.50
2.00
1.50
1.00
0.50
0.00

-0.50
-1.00
-1.50
-2.00

1.35
S (kg/m3)
25
24.8
24.8

U (1/)

1.8
1.6

0.15
0.27
u (1/h)

1.4
1.2
1
0.8
0.6
0.4

0.5

1.6
1.4
1.2
1
1.50
u (1/h) 0.8
1.54
0.6
1.51
0.4
1.48
0.2
1.49
0
1.48
0
-0.2
1.55
1.48
0.62
0.03 1.80
U
-0.04 1.60
1.40
1.20
1/U 1.00
0.80
0.67357950.60
0.64685510.40
0.67543330.20
1.60662420.00
34.9497854 0

24.6
24.3
23.3
20.7
15.7
10.2
5.2
1.65
0.2
0
0

1/S
0.0980392157
0.1923076923
0.6060606061
5
0

C
Ks/Umax
1/Umax

1.800 0.1987
1.600 0.6077
f(x) = 0.198688373
1.400
R = 0.9925431432
1.200
1.65
1.000
0.3269705447
0.800
0.600
0.400
0.200
0.000
0.000
1.000
2.00

Umax
Ks

YA=a*exp(-exp(b-c*TA))
Dep. Var. : YA
1.80

1/U

1.60

f(x) = 0.1986883732x + 0.6077361246

R = 0.9925431432

1.40
1.20
1.00
0.80
0.60
0.40
0.20
0.00

0.20
0.00

MODELO DE GOMPERTZ
a

2.041589

1.667028

1.053617

Umax= a*c

2.151052

CONCLUSION
Umax
Uprom
Umax Monnot
Umax Gompertz

1.55
1.35
1.65
2.15

Yxs
30
Concentracin (kgm-3)
25
0.05
200.00
0.50
150.50
0.53
10
0.42
50.46
Siempre debo
0.45
empezar0de
0 para
0.50
los cultivos
0
0.5
0.42
0.48
0.50
0.00

Concentracion
celulas / sustrato
Log (X/Xo)
YA
0
0.021189
0.041393
0.204120
0.371068
0.698970
1.021189
1.344392
1.537819
1.672098
1 1.736397
1.5
1.763428
1.767156
Concentracion de Celulas
1.763428

2.5

Concentracion de Sustrato

3.00

1/h)

Se halla Umax (velocidad

maxima) no es integral
perfecta.

2.50
2.00
1.50
1.00
0.50
0.00

0.5

1.5

2.5

3.5

-0.50
-1.00

Tiempo (h)

-1.50
-2.00

1.8

= ()/(+)

1.6
1.4
1.2
1
0.8

1/= (+ )/()

1/= / + /
()

0.6
0.4

3.5
Tiempo (h)

0.46

1/= 1/ +

1.6

1/= (+ )/()

1.4
1.2

1/= / + /
()

1/h)

0.8
0.6
0.4

1/= 1/ +
/1/

0.2
0
0
-0.2

10

15

20

25

S (kg/m3)

1.80
1.60
1.40
1.20
1.00
0.80
0.60
0.40
0.20
0.00
0

10

Chart Title

S (kg/m3

0
0
f(x) = 0.1986883732x + 0.6077361246
0
R = 0.9925431432
0
0
0
0
0
0
0
.000
1.000
2.000
3.000
4.000

5.000

361246

12

1/S

6.000

30

1/S

ato

3.5
Tiempo (h)

1/S
0.040
0.040
0.041
0.041
0.043
0.048
0.064
0.098
0.192
0.606
5.000

6.764
3.654
0.667
0.650
0.662
0.674
0.672
0.674
0.647
0.675
1.607

120
240
360
480
600
720
840
960
1080
1200
1320

Tiempo (d)

Concentracion de biomasa
x (gL-1 peso seco)

Concentracion de azucar
(gL-1)

0
5
10
15
20
25
30
35
40
45
50
55

0.64
1.95
4.21
5.54
6.98
9.5
10.3
12
12.7
13.1
13.5
13.7

30
27.4
23.6
21
18.4
14.8
13.3
9.7
8
6.8
5.7
5.1
Yxs global

Tiempo (h)

Log X (gL-1)

(1/h)

0
5
10
15
20
25
30
35
40
45
50
55

-0.19382003
0.29003461
0.62428210
0.74350976
0.84385542
0.97772361
1.01283722
1.07918125
1.10380372
1.11727130
1.13033377
1.13672057

0.0968
0.0668
0.0238
0.0201
0.0268
0.0070
0.0133
0.0049
0.0027
0.0026
0.0013

Umax

0.0968

S (gL-1)

30
27.4
23.6
21
18.4
14.8
13.3

0
0.0968
0.0668
0.0238
0.0201
0.0268
0.0070

Ks/Umax
1/Umax
Umax
Ks

9.7
8
6.8
5.7
5.1

0.0133
0.0049
0.0027
0.0026
0.0013

1/S
0.0333333
0.0364964
0.0423729
0.0476190
0.0543478
0.0675676
0.0751880
0.1030928
0.1250000
0.1470588
0.1754386
0.1960784

1/U
0.0000
10.3337
14.9590
41.9366
49.8278
37.3502
142.3949
75.3647
203.0665
371.2621
382.7759
782.8648

-4.5698
0.2585
3.8684719536

Yxs

Log (X/Xo)

0.50
0.59
0.51
0.55
0.70
0.53
0.47
0.41
0.33
0.36
0.33

YA
0.00000
0.48385
0.81810
0.93733
1.03768
1.17154
1.20666
1.27300
1.29762
1.31109
1.32415
1.33054

a) Representar U en funcion del tiempo de cultivo . Cundo se alc

b) Representar la velocidad especfica de consumo de azcar en fu
c) Cul es el rendimiento observado de biomasa a partir de sustra

Concentracion celulas/sustrato vs tiem

35
30
25
20

Concentracion gL-1

15
10
5
0

0.52

10

20

30

40

Tie
Concentracion de Celulas

Concentracion de Sustrat

Curva de Crecimiento Logaritmico

1.20
1.00
0.80

Ln X (gL-1)

0.60
0.40
0.20
0.00
14

16

18

20

22

24

Tempo (d)

Modelo de M
0.1200
0.1000
0.0800
0.0600
0.0400

0.1200
0.1000
0.0800
0.0600
0.0400
0.0200
0.0000
16

18

20

60.0000
50.0000
40.0000

f(x) =Chart
2446.753353502
Title
R = 0.9048429849

30.0000
20.0000

MODELO DE GOMPERTZ
a
b
c
1.293490

Umax

0.819133

0.17892907

0.138331

10.0000
0.0000
0.0350000

0.0400000

0.0450000

de cultivo . Cundo se alcanza la mxima velocidad de crecimiento?

de consumo de azcar en funcin del tiempo
e biomasa a partir de sustrato? Es Yxs constante?

lulas/sustrato vs tiempo

20

30

40

50

60

Tiempo (d)

ulas

Concentracion de Sustrato

iento Logaritmico

20

22

24

26

Tempo (d)

Modelo de Monnot

18

20

22

24

26

28

f(x) =Chart
2446.753353502x
- 81.351087215
Title
R = 0.9048429849

0
50000

0.0400000

0.0450000

0.0500000

0.0550000

0.0600000

MODELO DE MONOD
133

Concentracin

Tiempo (h)
0.00
2.50
24.00
30.50
48.00
78.00
96.00
168
171

Brix
23.7
23.6
23

N (*10^6)
15
25.25
35.55
29.85
37.45
63.75
110
5.9
19.4

21.5
20

15
15
15
15

Rendimiento global: Yxs global

Tiempo (h)
0.00
2.50
24.00
30.50
48.00
78.00
96.00
168
171

Ln X
2.708
3.229
3.571
3.396
3.623
4.155
4.700
1.775
2.965
Umax

S (kg/m3)
23.7
23.6
23
21.5
20

15
15

Concen
Sustrat

113
Yxs

0.0098
93
0.0583
73
-0.2632
0.1974
53
0.1901
33
0.0000
0.0000
13
0
0.0000
0.0000

Concen
Biomas

20

40

0.0916
10.91954023

U (1/h)
0.208
0.016
-0.027
0.013
0.018
0.030
-0.041
0.397
0.208

U (1/)

0.250

0.208
0.016
-0.027
0.013
0.018
0.030

0.250
0.200
0.200
0.150
0.150
0.100

f(x)
R =

0.100
0.050
0.050
0.000
14
0.000
-0.050
21
-0.050

16
21.5

33

Densidad

oncentracin

13

Concentracin de
Sustrato

93

Concentracin de
Biomasa

1012
1013
1014
1015

73

53

33

13

20

40

60

80

100

120

Tiempo (h)

Ln X

5.00
4.50
4.00
3.50
3.00
2.50

20

40

60

80

100

120

Tiempo (h)

HORRIBLE
Chart Title

0.250
0.250
0.200
0.200
0.150
0.150
0.100

f(x) = 0.0959468151x - 2.112213627

R = 0.6862862363

0.100
0.050
0.050
0.000
14
0.000
-0.050
21
-0.050

16

18
21.5

20
22

22
22.5

24
23

26
23.5

24

Baum
1.7
1.84
1.98
2.12

Brix

Alcohol

0.2
0.47
0.73
1.1

Rendimiento global: Yps global

0.11
0.23
0.43
0.59