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Specification Model: CS-400

Product compositionThe analytical part (host), operation part (computer system), the
result output part (printer), accessories and consumables.
Product applicable scope: used for quantitative analysis of serum, plasma, urine,
cerebrospinal fluid and other clinical chemical constituents of sample.

1-1-2 The Front of Analyzer

Machine model logo


Upper cover
Left front door
Right front door

1-1-3 Front Open Picture of Analyzer

SIP (reference), injection pump


DIL (Diluted Release) injection pump
IS (internal standard), injection pump
CS-alkaline cleaning liquid box
S injection pump
R2 injection Pump
R1 injection pump

1-1-4 The back of analyzer


Power entrance
RS232 interface
left back cover board
cooling fan
light liquid outlet
right back cover board
purified water entrance
concentrated liquid waste outlet
concentrated waste liquid level sensor interface

1-1-5 The Top of Analyzer


sampling mechanism

reaction cup cleaning mechanism


reaction disk mechanism
reaction bath liquid detection
R1 stirring mechanism
R1 reagent adding mechanism
probe cleaning reagent groove
R1 reagent disk
sample disk rotating indicator
sample disk inner refrigerated cover
sample disk
R2 reagent adding mechanism

R2 stirring mechanism
R2 reagent disk

1-1-6 The Right of Analyzer


Analytical unit switches
(not including refrigeration power supply)
refrigeration power supply indicatorgreen
main power supplybreaker
power indicatorred

1.2

Analytical Unit Composition

CS-400 auto-chemistry analyzer working speed means the one at which it reaches
constant speed 400 tests / hour of single / double-reagent item, whose working period is 9
seconds. Instrument overall structure adopts the "4 -disk + three-probe + two-stirring rod",
specifically, a sample disk, one reaction disk, two reagent disks, 2 reagent probes for
adding R1 and R2 respectively, a sample probe for sampling, 2 stirring rods for mixing
R1,R2 respectively. "Grating + diode array" approach is adopted in optical measurement
mechanism for real-time optical collection of reaction cup. The rinsing mechanism 7-stop
11-step automatically rinsing the reaction cup is carried out in test process.
1-2-1 The Structure of Analyzer

Sample disk

1-2-2 Sample Unit

Barcode window

115 sample positionsincluding


Routine sample position

50

STAT sample position

20

Standard solution position:

34

QC position

Cleaning liquid position

Barcode window

1-2-3 Reagent Unit


Reagent position452
R1diskonly for R1and R4
R2diskonly for R2 and R3
Position 45 of two reagent disk:
Only for phosphor-free cleaning liquid
Reagent bottle volume70mland 20ml

1-2-4 Reaction Unit


Reaction cup

Reaction disk

Reaction cup120optical path: 6mm


206 sets hard optical plastic
cup
Incubation bath
Digital liquid sensor
7-stop 11-step rinsing of colorimetric cup

1-2-5 Probe and Stirring Unit


Stirring mechanism

probe mechanism

Probe mechanism3
1sample probe, 2 reagent probes
High-precision digital liquid detector
Stirring mechanism2
High-speed hollow cup motor
Surface high-intensity Teflon coating

1-2-6 Precise Distributing Pump Injection Pump

ISE unit3SIPDILIS
Colorimetric unit3 samplereagent R2reagent R1
Colorimetric cup original rinsing liquid box

1-2-7 Control unit 1

circuit panel boxes: 6 panels


Order: from left to right
Reaction disk control panel
Sample disk control panel
Reagent R1 control panel
Reagent R2 control panel
ISE control panelOptional
Main control panel
Switching Power Supply Box4
Order: from left to right
12V12Vsimulation
12Vlamp
5Vdigital circuit
24Vmotor, valve
Halogen Lamp Power Interface

Circuit panel box power supply interface


24VPower Control Interface
halogen lamp power supply control interface
switching power supply (except +12 V (lamp))
communication control interface
fan interface

1-2-8 Control unit 2

Connection adaption and the status indicating board


AC electrical driver panel

1-2-9 Control unit 3

Semiconductor refrigeration systems:


Control panel (with status indication)
+5 V panel power
13.5V Power Supply
cooler
Cooler (with 4 fans
Cooler display:
E1 refrigeration temperature, E2 machine internal
temperature C1C4 4 Cooler current
AC circuit breaker: 5
Fan (4A)
4 communication pump (6A)
2 Heater (10A)
4 Switching Power Supply (10A)
Refrigeration system (10A)
Isolating transformers
Arrester panel

1.3

Function Overview

Main work flow


1. All mechanical moving parts unit initialization.
2. 4 times water blank measure is implemented after the fifth time rinsing of 7 times
automatically rinsing of reaction cup.
3. Sample assimilates quantitive sample when it descents to sample disk after the
sample disk rotates to designated sampling position.
4. After 7-stop 11-step cleansing, reaction cup stops at the sampling position, and
sample probe rotates to reaction disk and descends to reaction cup to discharge
it, and sampling finished.
5. RI reagent probe descents to R1disk to assimilate quantitive reagent when the
reagent disk rotates to designated position.
6. R1 reagent probe rotates to reaction disk and discharges reagent R1 when the
reaction cup finishing sampling rotates to R1sampling position.
7. Reaction cup finishing sampling R1 is stirred immediately when it rotates to
R1stirring position.
8. sampleR1 reagent are reacting or temperatured.
9. If it is double item test, R2 reagent disk rotates to the designated R2 reagent
position and R2 reagent probe descends to R2 reagent disk to assimilate
quantitive reagent after a set period (one minute plus 30 seconds).
10. The R2 reagent probe discharges the R2 into reaction cup when it rotates to
reaction disk after reaction cup rotates to R2 sampling position.
11. Finishing sampling R2 reagent, reaction cup is stirred after its one circle (2
patches) rotation.
12. Reaction cup carries out the collection of absorbance data when it passes the
optical unit in every period.
13. The process of sampling R3,R4 reagent and sampling R1, R2 reagent.the
same system for R1R4the same system for R2R3
14. R3 sampling position is used frequently instead of R2 sampling position for
double reagent item test so as to prolong the temperaturing time of sample and
R1 reaction liquid for 5 minutes. 10 minutes elapses in the process from
sampling to adding R4.
15. The reaction cup finishing reaction is rinsed automatically when passing the
rinsing unit, and 15minutes elapses since sampling to rinsing.

Table 3-1-1 Main Function of Each Unit

Name

main function

Sample probe unit

Execute sample assimilation and discharge of all biochemical


items and ISE items

Sample disk unit

Total 115 sample positions for carrying all test samples,


standard solution and QC liquid.

R1probe unit

Execute assimilation and discharge of all biochemical items


R1, R2 reagent.

R2 probe unit

Execute assimilation and discharge of all biochemical items


R1, R2 reagent.

Reagent
unit

R1

disk

total 115 reagent positions for carrying R1, R4 test reagent


and detergent

Reagent
unit

R2

disk

total 45 reagent positions for carrying R1, R3 test reagent and


detergent

Reaction disk unit

Total 120 reaction cups used as container of reaction and


colorimetry test.

Reagent R1 stirring
unit

Stirring when reagent R1, R4are added into reaction cup.

Reagent R2 stirring
unit

Stirring when reagent R2R3 are added into reaction cup.

Optical
groupware

Measure 12 wavelength absorbance by grating system

system

Auto-rinsing unit

Rinse reaction cup automatically by 7-stop 11-step

ISE unitoptional

Carry out ISE measurement (KNaCl

barcode

Total 3 for scanning reagent bottles in the two reagent disk


and sample cups in sample disk.

10

Chapter 2 Instrument Installation

2.1 Installation Space Requirement


To make sure the space of maintenance, operation and repair, please follow the instruction
as below:
Space between left (right) side of analyzer and the wall should 50cm
Space between rear board of analyzer and the wall should 50cm
Space in front of analyzer should100cm
Make sure there is enough space for waste device and purified water
equipment.
2.2 Power supply requirement
Power supply: ~220V, 50Hz
Power: 2000 VA
Circuit breaker: 250V, 20A
Instrument is equipped with a three core electrical wire, red wire is live line, blue wire is zero line,
and yellow green wire is ground lead as figure 1 shows.

Figure 1 i
A well grounded power supply socket is a must. (Socket at least with one 20 A and three 5A).
Large electrical appliance such as air condition, refrigerator, even cannot use the same
electrical wire as analyzer.

11


! Warning:

Incorrect earthing may cause electric shock or instrument damage.

Input voltage should conform to requirement. 6KVA-line UPS power supply is advised.

2.3 Environment requirement


Working environment: 1532
Relative humidity: 4085
Atmospheric pressure: 76kPa106kPa
Environment should be with no dust, mechanical vibration, and noise source and
power interference
Do not put the analyzer in the vicinity of brush motor, flicker fluorescent tube and
other constant on-off electrical equipment.
Hard and flat enough the ground should be to stand the instrument.
Avoid direct sunlight, do not put the analyzer in front of heat source and wind source
Keep good ventilation of the instrument.

! warning

Normal running and accuracy of result can not be guaranteed if instrument works beyond
the requirements mentioned above. Please use air conditioner if the temperature or
humidity can not meet the requirement above.

The heat generated in the work process by the instrument will be emitted the rear of the
instrument, so good ventilation should be kept well and ventilation equipment can be
adopted if necessary, but direct air current is avoided, or inaccuracy of instrument test may
be caused.

4.2 2.4 Purified water equipment Requirement


water should be obtained from tap water pipe
water conductivity should within 1uS/cm
water supply volume should reach 40L/h or more
The hydraulic pressure should within 49-343 Kpa
2.5 Instrument Installation Flow

Make sure the installation place, space, electrical environment, installation room
temperature and purified water equipment can conform to requirements

Make sure instrument installation tools needed are complete and reagent and QC liquid are
enough.

Please check the prepared items according to packing list when open the package; please
write them down on the check report if any missing.

12

Place instrument in applicable position, and mount with computer host, display and printer.

Connect water supply and waste liquid outlet equipment.

Adjust instrument level, and check whether the injection pump wires are loose or not after
open the left and right cover board of instrument.

Infuse CS-alkaline detergent into instrument rinsing box, and infuse CS-anti-bacterial
detergent into the 45th position of R1, R2 reagent disks.

Replenish cooling system water tank with purified water.


aSwitch off the main power
bDemount instrument left front cover board as figure 2 shows:

cUnplug the cork of the two hoses connecting to cooling system water tank as figure 3 shows

High water level hose

Low water level hose

13

dInfuse purified water into low water level hose till the purified water flows out of the
high level hose.
eSwitch on the main power. After several minutes,

continue to infuse purified water

into low water level hose till the purified water flows out of the high level hose again,
requiring 3L water.
fReplug the rubber cork and mount the left front cover board of the instrument.

Check whether power supply and data wires are connected.

Mount reagent probe, sample probe, reaction cup.

Check the up and down flexibility of reagent probe and sample probe.

Get through pure water machine, computer host and display and analytical unit power
supply, and enter CS auto-chemistry analyzer systematic application software. Initial user
name: 001, initial password: 001.

After enter software, follow the steps below in Maintenance interface.


aInjection pump exhaust
Execute injection pump exhaust to expel air in pipeline.
bCleaning liquid pipeline exhaust
Executing irrigation cleaning liquid pipeline exhaust is infusing cleaning liquid into
pipeline to expel air in pipeline.
cReagent probe horizontal check
Make sure reagent probe is right above reaction cup, rinsing groove and reagent bottle.
dsample probe horizontal check
Place a standard cup at position C8 in the sample disk outer track, middle track and
inner track respectively, and make sure the sample probe is above reaction cup, rinsing
groove, standard cup by implementing sample probe horizontal check.

14

eStirring rod horizontal check


In order to make sure the stirring rod is above the reaction cup, rinsing groove.
fMechanical movement check
Execute 20 times mechanical movement checks to make sure whether the washing
block of rinsing mechanism nozzle abrases the reaction cup or not and each
mechanism runs normally or not.
gRinse reaction cup+ ISE
Select rinsing reaction cup in Maintenance interface, and execute rinsing reaction cup
+ ISE if ISE equipment is collocated.
hLight quantity check
Light quantity result should be attached to installation check report with its value no
more than 18000.
iCup blank test
No. 1 cup blank value should be within 18000, and 2-120 reaction cup check value
should be within 18000 800.

2.6 Clinical item test


Edit chemical parameters; register reagent info.; testing rate assay ALT, point assay,
two-point rate BUN; calculate the difference of parameter and the result

of test should be

attached to installation check report.

2.7 Train Medical Personnel

2.8

Fill the Installation Check Report Detailedly.

15

Chapter 3 Performance and Test Flow

3.1

3.1.1

Main Performance Index

Instrument standard specification

Performance Index

characteristics

Wavelength
range

Grating
rear spectrophotometry system,
Simultaneous photometric processing of 12
wavelengths within the range340 380 405
450 480 505 546 570 600 660
700 750nm

Wavelength
precision

2nm

Reaction
temperature

370.1

Test item

Sample system

standard specification

Simultaneously testing 88 colorimetric items and 3


ISE items at most

Measuring
method

Rate assay ,end-point assay, 2-point assay.

Measuring
speed

Constant speed, 400 tests/ hour ( 640 tests/ hour


with ISE)

Sample disk
and position

Total 115 positions(routine sample: 50, calibrator:


34, STAT: 20, QC liquid: 8, cleaning liquid: 3) in
inner, middle, outer circle.

Sample type

Serum, plasma, Urine, cerebrospinal fluid, ascites


and other body fluids

16

Sample
volume

235ul0.1l Incremental
Test tube 10mm75mm 10mm100mm

Sample cup
specification

13mm75mm13mm100mm(1 mm)
Standard cup 14mm37mm(1 mm)

Sample system

Remaining
sample
volume

More than 100l

Sample
probe

Appropriative probe with liquid detector,


bump-proof function and probe clot detection
function.

Sample
probe rinsing
Sample liquid
level sensor

Inner, outer wall rinsing


Digital liquid detecting, integration with sample
probe
Type: code 128 code 39 code 93 12of5
UPC/EAN

Barcode
info.

Reagent system

Sizewidth: 812mmvalid length within 40mm,


start blank and finish blank within 3mm when
cutting.
Sticking requirement: the lower edge of barcode
should be within 15 mm 20mm away from the
test tube bottom to make sure right reading
barcode, and make sure the barcode is aligned
with sample position gap when putting the test
rack.

Reagent disk,
reagent
position

R1 and R2 reagent disks with two refrigerator,


adopting a semiconductor refrigerating, 45 reagent
positions for each reagent disk (the 45th fixed for
CS- anti-bacterial
phosphor-free cleaning
liquid.

Reagent
volume

20350ul1l

Reagent
bottle
specification
Reagent
remaining
volume

(incremental)

20mL 70mL

Reagent liquid volume: more than 3 ml

17

Reagent
probe
Reagent
probe rinsing

Inner and outer wall rinsing

Reagent
storage
temperature

All reagent should be stored at 515

Reagent liquid
level sensor
Reagent system

Digital liquid level detecting, integration with


reagent probe
typecode 128

Barcode
info.

Reaction system

R1R2: using special probe respectively with liquid


level detector and bump-proof function.

size: Sizewidth: 812mmvalid length within


40mm, start blank and finish blank within 3mm
when cutting.
Sticking requirement: the lower edge of barcode
should be within 15 mm 20mm away from the
test tube bottom to make sure right reading
barcode, and make sure the barcode is aligned
with sample position gap when putting the test
rack.

Reaction cup
mode

Discrete

Reaction cup
optical path

6mm

Reaction cup
quantity

6 sets, 20 for eachtotal 120.

Reaction time

15 minutes at most, 34510 and 15 minutes


available

Reaction
liquid
volume

150450ul

Light source

20W/12V Long-life quartz halogen

Absorbance
range

03.3ABS

QC
Automatical
rinsing

QC interval, monthly QC
Automatically rinsing reaction cup, reagent probe,
sample probe, stirring rod.

18

Stirring
system

Separately stirring after adding reagent

interface

TCP/IP network interface, standard RS-232 and


USB 2.0 interface.

Printer

Stylus printer, supporting the user-defined mode for


report sheet

Data system

Connecting
LIS/HIS
system

LIS/HIS system available

weight

Approximate

Dimensions
Instrument system

1060 mm790 mm1150mm(lengthwidthheight)

PowerVA

2000VA

Water
consumption

25L/h

Power supply

Installation
requirement

3.1.2

Using
environment

300Kg

220V/230V50Hz/60Hz2000VA
System storage temperature:0 40
volatility <2/Hstorage humidity:30%RH
80%RH non-condensing at working,
temperature:15

30

volatility<2/H

at working, relative humidity:35%RH


80%RH non-condensing not higher than
2000 meters above sea level

Testing speed

NoteDue to the different specific conditions, sometimes equipment processing capacity will be
lower than 400 tests / hour.
Test conditions
Retest
after
prediluted

sample

Use
avoiding
cross
contamination function
R1 and R4 items or R2 and
R3
items
are
used
simultaneously in testing.

Degree of reduced ability to process (estimated)


133tests/hall tests after redilution
200 tests/h at leastreaction cupsample probe
200400tests/h reagent probe
200tests/h at least

19

Test Flow
3.2

Typical test flow


3.2.1

figure 3-1 test flow

Stop automatically
Rinse reaction cup

Tested completely
stir

1min
plus
21s

13min 3os

Add reagent 4
stir
Add reagent 3

stir
Add reagent 2
stir
Add reagent 1
Add sample
Assimilate water

3min plus27s
18s
More then72s

4 times of measuring water blank


Rinse reaction cup
Initial running(Reesetting)
Start

15min
3min

9s

4 min plus 57s

9min 18s

9s for one additional sample

Total time for testing the 1st sample: 18min

20

3.2.2

Test Flow Instruction

3.2.2.1

Periodic movement sequence of sample probe


a. Internal and external wall cleaning

b.Rotate to above sample disk and assimilate 2ul air.


c.Descend till the sample probe point into liquid level more than 2mm
d. Assimilate quantitive volume + push back redundant sample
e.Rise from sample test tube and rotate to above reaction disk
f.Descend into reaction cup to discharge quantitive volume of sample
g.Rise and rotate to above rinsing bath
(next periodic movement sequence).
3.2.2.2

R1R4 reagent probe periodic movement sequence :

a. Internal and external walls rinsing


b. Rotate to above reagent disk and assimilate 5 ul air
c. Descend till the reagent probe point into liquid level more than 2mm
d. Assimilate quantitive volume +

redundant volume of R1 and

R4

e. Rise from sample test tube and rotate to above reaction disk
f. Discharge quantitive volume of R1 and R4
g. Rotate to above rinsing bath
(next periodic movement sequence)
3.2.2.3

R2R3 reagent probe periodic movement sequence:

The same to R1
3.2.2.4

Stirring rod periodic movement sequence

a. Rotate to above reaction disk


bDescend into reaction cup
cMix reaction liquid
dRise from reaction cup and rotate to rinsing bath
eDescend into rinsing bath
f Stirring rod rinsing
gRise from rinsing bath

21

3.2.2.5

Movement and time sequence of reaction disk

A track includes total 120 reaction cups in reaction disk, and rotates in a fixed way when
testing. The reaction cup always rotates and stops 3 times counterclockwise, total 2237
2=61 rotation and stop sequence 22372patches, in every working period, 9
seconds elapsed. 122 patches are passed in two working periods within 18 seconds.
figure 3-2 Position of reaction disk and probe

Rinsing mechanism

Reset point

Position No. of reaction cuvette.

Photoelectric
detection

Sop and wipe

62,R1 stirring po
63,R4 stirring pos

Sample probe

R1, R4 Probe
60 R1 Pipetting
61 R4 Pipetting

Reference position
Reagent probe 2.3

Stirring rod

number

Outer circle figure: No. of reactioninner circle figure: No. of mechanism position;
Sample position: No. 1 position; reagent 1,4 probes: No.60,61 position; stirring at
NO.62,63 position; reagent 2,3 probes: No. 31 position, stirring: No. 33 position; reaction
cup rinsing mechanism: No. 10110310510710911711 position.
Reaction cup stops at No. 101 position after reaction disk resetting. The sequence of
reaction cup rinsing and sampling:
162364566768 591209 minutes60times
612634656678 119609 minutes60times

22

3.2.3

figure 3-3

Reaction Cup Rinsing Movement Sequence

Reaction cup ri nsing probe position

Rotati
Rotationa
l
l
direction
f

4 4

times 1
cell
blank

measurement.
1 stop, 3 pass.

Above figure shows that seven steps are needed when rinsing reaction cuvette. (four times
cell blank test is added) , therefore, to finish rinsing one reaction cuvette, 11 steps are
needed

23

3.2.4

Optical measurement movement sequence

Photometry in the entire process is adopted. In 15-minute reaction time, the continuous
determination of the absorbance of reaction solution is carried out. Reaction disk rotates 1
plus 2 pitches, about 18 seconds, absorbance values are measured out when the 120
reaction cups passing optical axis of the photometer one by one.
Each reaction Cup in 3-minute reaction time was measured 10 times (10 photometric
points), 4-minute reaction time was measured 13 times (13 photometric points), 5-minute
reaction time was measured 16 times (16 photometric points), 10-minute reaction time was
measured 33 times (33 photometric points), 15-minute reaction time was measured 49
times (49 photometric points).

Detector
(340-750nm)
12
wavelength

fixed

3-4 Optical system


Light
source

Reacti
on

lamp

cuvett
e

Figure 3-4

Photometer

Light starting from the light source was focused by the lens, and passed the reaction cup
first, and then was disparted by concave grating. After spectrophotometry, each
wavelength were received by 12 fixed photoelectric sensor simultaneously, and were
amplified 12 amplifier, after Log transformation to derive the rate of change of absorbance
or absorbance. When dual-wavelength testing is used, the concentration value is
calculated by the difference of the main and sub-wavelength absorbance or that of
absorbance change rate, and therefore dual-wavelength testing can not only compensate
the blood lipid, hemolytic, jaundice sample test, but also compensate on the result
impacted by voltage changes, so that measurement is more accurate, more stable.

24

Chapter 4. Module Introduction

4.3
4.3.1

Sample / reagent probe unit


Function introduction

Sample/reagent probe unit includes sample probe, No. 1 sample probe(R1) and No.2
sample probe (R2), which are called 3-probe component.
Sample probe can realize the assimilation from the sample test tube and sampling into
reaction cup. No. 1 sample probe(R1) and No.2 sample probe (R2) can realize the
assimilation from the reagent bottle and adding reagent into reaction cup.
In addition, main function of 3-probe component: liquid level detecting and bump-proof in
movement process, sample probe block detecting function.
Other subsidiary function includes mechanical limit, power-down self-locking function.
3-probe component working position
1. sample probe component rinsing bathsample
positionreaction disk/ISE sampling position

disk

assimilating

2. reagent probe component rinsing bathreagent


positionreaction disk/ISE adding position

disk

assimilating

Probe drive mechanism plays a key role of reagents and samples adding. The way of
probe are only up-down and circular moving, so two step motors are necessary to drive.
4.3.2

Composition and configuration

Figure 4-1 probe components configuration


Probe rotating arm
Probe rotating gear
belt
Probe rotating step
motor

Probe up-down light


sensor block tablet

Probe up-down
weight block
Probe up-down
guide slider

Probe up-down step


motor
Probe up-down
gear belt

Reagent 1, reagent 2 and sample probe drive mechanism in addition to different probe
turning angles, namely the corner mechanical limit block tablets different, the other
structures are identical.

25

figure 4-2

Probe body and rotating body

Rotating motor
mounting hole

Rotating fixed light


sensor position

Guide
movements
long hole

Up-down
motor
mounting
hole

Lower rotating axle


center

Probe rotating gear

Probe
rotating
knight head

lower probe rotating


axle

Rotating probe drive ratio 12:34, using 0.9 stepper motor and 8 segment controller.
Control accuracy can achieve 0.0398.
Up-down main gear driver diameter is 19.1mm, the 60mm for the perimeter, also using 0.9
stepper motor and 8 segment controller. Up-down control accuracy can achieve
0.01875mm.

26

4.4

Rotating mechanism unit

4.4.1

Function Introduction

The main function of rotating mechanism is bearing of the sample warehouse, reagent
warehouse and reaction disk, and drive it to rotate, so that sample, reagent carried in
reaction cup rotate to the designated location to finish sampling, mixing and other work.
Reagent disk 1, reagent disk 2, the sample disk and reaction disk mechanisms are classified
as turntable mechanism. Meeting the requirements of functionality and performance
simultaneously, in order to improve the craftwork of product, the four disks are designed as the
same frame structure.

4.4.2 Rotating mechanism configuration


1, the sample turntable: The sample storehouse, disk rotating bracket, step
components

motor-driven

2, reagent turntable: The reagents storehouse, disk rotating bracket, step motor-driven
components
3, the reaction disk turntable: reaction plate, the reaction cup, incubation bath, disk rotating
bracket, step motor-driven components
Figure 4-3 Disk rotating bracket

Mandrel

Storehouse
t

fixing

S/Driving seat
Zero light sensor

Storehouse cover light sensor

Code disk light sensor

Reaction disk motor


seat

The foot of bracket

Reagent disc 1 and disc 2 are same the structure , and the sample disk drive structure and
the drive transmission ratio are the same with reagent disc.

27

Figure 4-4 sample/reagent disk motor driver configuration

Driven gear

Code disk and light


sensor

Drive gear belt

Driving step motor

System transmission ratio is 10:1.Because of using 0.9 step motor with 8 segment driver
circuit, the wheel rotation accuracy can achieve 0.01125 .

Figure 4-5 Sample/reagent disk assembly configuration

Leader of disk cover touch


switch

Connecting loop of
disk cover

Connecting loop reagent box


rack or sample tube disk rack

28

Figure 4-6 sample/reagent disk transmission code disk configuration


The only difference of drive structure of reagent and sample disk is the code disk.

45 teeth of reagent
code disk

Disk cover switch


light sensor

Original point block


and light sensor

Sample code disk


50 teeth of outer
track ,inner track
40 teeth

Original point light


sensor

4.5

4.5.1

Cooling mechanism

Function Introduction

R1 and R2 with two refrigerated reagent disk, adopting semiconductor refrigeration, the
temperature maintains at 6 degrees -10 degrees, 45 reagent positions for each reagent
disk respectively(the 45th fixed position for placing phosphor-free CS-anti-bacterial
cleaning liquid in each disk)

29

4.5.2

cooling system configuration and installation

Figure 4-7 reagent cooling storehouse reagent box rack assembly configuration

Equipped
temperature-keeping
cooling storehouse

with
reagent

Cooling water circulation


outlet
Reagent box
Barcode reader
scanning window

30

Figure 4-8 reaction disk assembly configuration

Gear drive is adopted to reaction disk due to the relatively high positioning precision.
Fixing pin
Colorimetric cup
rotating rack

Driven gear

Driving gear

motor vibrator

Figure 4-9 reaction cup installation disk assembly configuration

120 colorimetric cup


120 teeth of code disk in
colorimetric cup position

cuvette
cuvette install pin

Locking screw of
colorimetric cup rack
6 sets of colorimetric

31

Figure 4-10 Incubation bath components configuration

Liquid level sensor


rack

R1 stirring rod
rinsing bath
R1 probe rinsing
bath

Optical
window

Circular constant
temperature water
outlet

Spilling outlet of
incubation bath
Circular constant
temperature water
inlet

Figure 4-11 reaction disk with incubation bath components assembly configuration

Incubation bath

Phosphor-free
detergent inlet

Figure 4-12 reaction disk and optical system components assembly configuration
Optical system

32

4.6

Stirring unit

4-6-1

function introduction
Stir and mix reagent after adding it
Reagent 1, reagent 2 agencies are identical in addition to the mixing angle of rotation,
namely the code disk is different.

4-6-2

Stirring components configuration and installation

Figure 4-13 Stirring components configuration


Mechanism rotation
mechanical limit

Rotation code disk


and light sensor

Stirring mechanism
up-down motor
Stirring mechanism
up-down slider

Rotating mechanism of stirring and rotating arm adopts the direct drive way of
step motor output axle, using 8 segment drive circuit of 0.9 motor, and the control
accuracy can achieve 0.1125 . The largest angle is limited by the open angle of
rotatation code disk mechanical limit. And positioning is determined separately by
the left and right light sensors.
Figure 4-14 stirring up-down driver configuration

Stirring mechanism
up-down light
sensor

Stirring mechanism
up-down motor

Up-down slider axle

Curve axle and curve handle drive is adopted by stirring up-down mechanism .

33

Figure 4-15 stirring up-down slider drive configuration

Up-down linear
sliding axle
Up-down
guide axle
Up-down
slider

Because of mixing body movements are used by way of the crankshaft crank,
so the movements of the positioning accuracy at different locations different.
Landing and taking-off between the location of the speed of the highest, lowest
accuracy, and precision at both ends of the highest, the lowest speed.
Electrical axis from the axis of the slider bearings the size of 16.5mm, crank in
horizontal position, the landing position accuracy of 0.032mm.
Due to curve axle and curve handle drive is adopted by stirring up-down
mechanism, the up-down positioning precision are different at different position.
The speed is the highest and precision is lowest at the middle, however the
precision is highest and speed is lowest at the two ends.
The size of Motor axis from the axis of the slider bearings is 16.5mm, when
curve handle locates horizontally; the up-down position precision is 0.032mm.
Figure 4-16

stirring mechanism up and down positions

Up-down
slider
Up-down
slider

34

4.7

Colorimetric cup rinsing mechanism

4.8

Figure 4-17

Colorimetric cup rinsing mechanism

Rinsing probe
rack of
colorimetric cup

Up-down
step motor

Rinsing probe of
colorimetric cup

Up-down slider

Only one-dimensional movement available to colorimetric cup rinsing


mechanism, and driving mechanism adopts the up-down driving mechanism of
stirring rod.

35

4.9

Rack
Rack includes the main rack, electrical, gas liquid valve and water tanks and
other racks.

Figure 4-18 Main rack configuration


Supporting board of upper
cover

Standing
supporting board

Main worktable
board

Cable leading
groove
Isolating
standing
board

Adjusting foot

All-directional
caster

Figure 4-19 electrical rack configuration

Water-proof cover
board

Electrical
box

control

Cooling controller

Isolated
transformer

Power switch
rack

Electric rack is mainly consisted of control panel box, power rack and
waterproof board and such parts.

36

Figure 4-20 gas liquid valve rack configuration

Gear pump pressure gauge

Waste liquid separating tank

Water inlet

Waste liquid outlet

Vacuum tank

Vacuum pressure switch


Vacuum pump

Figure 4-21 Valve rack, gear pump rack and the main rack assembly.

Gas liquid valve main rack

Gear pump rack

37

figure 4-22

Water-in tank unit configuration

Heating water tank

Pressure gauge
Vacuum exhausting
tank
Magnetic pump

4.10

Optical system
CS-400 adopts advanced flat field grating photometer. Concave holographic grating
photometer is today's domestic and foreign advanced optical system with simple
structure, high optical efficiency, and signal to noise ratio and measurement speed
of machine have greatly been improved, compact photometer dimension, stable
performance, long life, and such highlighting advantage, achieving the requirements
such as multi-wavelength, multi-item simultaneously testing at high speed,
multi-wavelength (12) simultaneously collecting signals, a relatively large aperture,
good imaging quality and post-spectrophotometry.

Figure 4-23 Optical assembly


Bunching lens set

Photoelectirc
cable array

Water-cooled
light source
Diaphragm

Flat field grating

Log applifier

38

Chapter 5. Instrument liquid and Gas Line.

5.1

Main function of liquid line


The CS400 liquid line system can be divided into five parts: water inlet tank,
refrigeration, 37 degrees centigrade temperature, colorimetric cup cleaning, the
inner and outer arms and stirring rod cleaning.
1.

CS400 liquid line system includes sampling subsystem and cleaning


subsystem.

2.

Sampling subsystem uses three probes plus two stirring rods and three
injectors. The sampling injector uses 100uL, and the two reagent
injectors use 500uL.

3.

The inner and outer wall cleaning of three probes and two stirring rods
uses barotropic driving.

4.

Cleaning bath: five cleaning bathes plus waste liquor in the reagent
storehouse, together with the flooding waste liquor in the reaction disk
are seven kinds of routine waste liquor.

5.

The reaction cup cleaning uses the way named 7-stop 11-step.

6.

Water supply: uses the special outboard water-supply equipment and


the special water-supplying machine.

7.

Waste liquor: use plastic hollow main pipe whose inside diameter is 20
to 30 mm, and the wall thickness is 3 to 5 mm. The installation of the
main waste liquor pipe is height limited, and it requires the height is
helpful to the waste liquor entering the low concentration liquid buffer
vessel by its self-weight. As to the high concentration waste liquid, it is
providing liquid level sensor interface and outboard high concentration
waste liquid barrel.

8.

Source of power: the power of cleaning comes from the magnetic


pump..

9.

The cleaning of reaction cup should use two kinds of detergent..

10.

The vacuum degree for vacuum pump assimilating is between -28k and
-35kPa.

11.

The inner and outer wall cleaning of three probes uses independent
solenoid valves while the two stirring rods use one solenoid valve
together.

39

5.2

Liquid Line Principle

Figure 5-1

CS400 liquid line sketch map

40

Figure 5-2

liquid line of water inlet tank

1, when the low water level float detects out the signal, open the inlet valves
SV8, and water tank begins to be infused water until the high water level float
detects out signal, then turn off SV8 to stop the water. Influent flow is as follows:
Alarm of low water
level check

influent water

Alarm of

high water level


check

Stop influent
water

2, when the low water level float did not detect out signal, the water tank heater
began to work, and temperature control started. Magnetic pump began to work
simultaneously.
3, Output water pressure of water tank is controlled by the magnetic pump and fixed
damper regulator. Magnetic pump head is 8 / 11 m. Control water pressure is around
0.8kgf/cm2.
4, The outlet of tank water leads directly to the cleaning and incubation bath; the
other output water through gear pump supercharger to the pressure 1kgf/cm2 to 3
line probe mechanisms to rinse inner wall and pipeline, with exhausting device of
probe liquid line.
5, SV13 valve remains closed status so as not to affect water pressure during the
normal use of instrument. Only in the implementation of the maintenance of water
tanks, open SV13 valve, at the same time, time switch of SV8 inlet valve to empty
impurities in a water tank.
6, when abnormality occurs to the high and low water level floats, possibly water
tank remains the status of inputting water. When the water tank is full of water, SV8
not shut down, tank outflow water spills out from the overflow pipe into the waste
liquid barrel through waste liquid pipe.

41

Figure 5-3 Semiconductor cooling refrigeration liquid line

1.In the system, two reagent disks as well as standard and QC sample need to be
cooled in the environment from four to ten degrees centigrade. Water is used to
exchange temperature and transfer heat.
2. the system also adopts the semiconductor refrigeration mode which is better for
environmental protection and convenient for maintenance than the traditional.
3.each refrigeration unit is made of four groups of same semiconductor refrigeration
module string by parallel connection. Each module is constituted by one piece of
Peltier (semiconductor refrigeration), one water cooling block and one radiator.
4.the refrigeration power of each module is about 80W, the total 320W. Radiation
adopts pyrotub wind-cooled heat pipe to radiate. The single power is 150W.
5.the refrigeration warehouse shell with two reagent disks adopts stainless steel
structure, and the cold water flows into it from the bottom and flows out from the top
through circumvolution circulation, thus the warehouse interlayer is full of cold water
and its temperature is even.

42

6.the refrigeration volume of sample disk is small and the refrigeration area is
formed by aluminum cylinder, so the aim of refrigeration can be achieved by
circulating stainless steel tube. The whole circulation process is finished by magnetic
pump with a head 2.7m.
7. sluice water tank can check temperature and water level. The scope of
temperature is from 5 to 15 degrees centigrade above zero.
8.the refrigeration water tank is complete closed.
When adding water, it should be added from the inlet tube at the bottom by filler.
About 1.5 litter is added into water tank or flowing-out water from the top of water
tank observed (the two water-changing nozzle should be above the water tank,
water-changing tube is mainly used to exhaust). Here, turn on the main power
supply and the refrigeration system starts to work. Then the water level will drop,
water should be added continuously until the exhausting tube reflows out water.
When working stably, there is no change with the water level of exhausting tube, and
the two water-changing tube plugs should be installed. If there is no leakage, the
system water changing has been finished.
When spouting the water, please turn off the main power supply, pull out the two
water-changing tube plugs and contain water with container. The total water volume
is approximately four litters.

Use the fresh water to do the refrigeration circulation, check it annually.

43

Figure 5-4 constant temperature system liquid line


This system offers precise constant 37 degrees water to the incubation bath of reaction disk,
and cools the high temperature light source simultaneously. This system consists of magnetic
pumps, inlet valves, release valves, liquid level detector and temperature controller which
consists of the heater temperature

1, Open the inlet valve SV10 and turn off outlet valves SV16 to infuse water into
incubation bath, simultaneously with reagent R1 and R2 probes adding
phosphor-free anti-bacterial rinsing liquid to the incubation bath, liquid level detector
determining whether to stop water.
2, Turn off outlet valve and inlet valve, and start the water circulation magnetic
pump and temperature controller. In order to improve the adjusting performance of
the PID temperature controller in high temperature environment, the system is
added the cooling device through the water tank to get a small amount of
temperature cooled.
3, Turn off magnetic pump and temperature controller, open the drain valve SV16,
time to turn off the drain valve when the incubation bath is draining.

44

Figure 5-5 probe internal and external walls rinsing and sample probe block check liquid line.

1, Opening the valves SV1 and SV41 simultaneously can get the inner wall of
sample probe rinsed; open valve SV2 or SV3 to carry out reagent probe R1 or R2
internal wall cleaning. Probe position should be at the top of the corresponding
cleaning trough when cleaning so that waste liquid can get out of the instrument.
2Open the valve SV4SV5SV9 or SV6 to rinse the external walls of sample,
reagent R1, R2 or 2 stirring rods. Each stirring rod has one corresponding valve
respectively. Fixing pressure adjusting piston is adopted to every external wall
rinsing pipeline to avoid rinsing water spilling out of rinsing bath.
3, valve SV1 pressure testing and the valve SV41 constitutes series structure, not
only completing the sample probe cleaning of the inner wall, but also completing the
block detection of probe, which does not affect the accuracy of assimilating and
discharging liquid volume of a small amount of sample.
4. The block detection must be implemented when probe is cleaned. Turn off valve
SV1 after the cleaning, Detect pressure, and probe is completely blocked or partially
blocked if the pressure value change is the same or smaller than the range value.
And previous added sample should be deserted and alarm is issued. Turn off SV1
and SV41 when probe assimilates and discharges sample.

45

Figure 5-6 Colorimetric cup rinsing liquid line


In order to achieve the cleaning effectively, colorimetric cup rinsing adopts warm
water. In order to improve cleaning speed, colorimetric cup adopts hydraulic valve
switch and vacuum liquid exhaust.

1, warm water provided by heating tank of inlet water is about 35 . Water


pressure is produced by magnetic pump from the water tank and adjusted by
voltage regulator to the stable pressure, about 0.6kgf/cm2.
2, vacuum pump, vacuum tank and pressure detector composes vacuum source
with pressure value about -0.8kgf/cm2. Vacuum tank has some fluid, gas
separating role in order to guarantee the safety of vacuum pump besides having
stable vacuum pressure.

46

3, There is a device on the vacuum tank to eliminate air bubbles with liquid, gas
separating bottle.
4, vacuum liquid discharging, having concentrated and diluted liquid passages,
consists of the valves SV21, SV30, SV31 and concentrated, diluted liquid
separating bottles. Concentrated liquid means reaction liquid, including relatively
concentrated sample and reagent of patients, requiring separate collection.
5, when the colorimetric cup cleaning mechanism descends, turn off valves SV30
and SV31 first, and then open the valve SV21. It tarts assimilating sample under the
vacuum pressure, and the liquid of colorimetric cup will be discharged after a short
period of time when the rinsing mechanism arrives at the bottom of Colorimetric
Cup.
6, cleaning mechanism begins to add cleaning solution and ionized water, turn off
valve SV21 after the addition is completed, and then open valves SV30 and SV31.
At this time, the waste liquid discharged into isolating bottle outflows by its gravity.
7, cleaning liquid and ionized water adding is completed by the five valves SV42,
SV43, SV14, SV15 and SV11 which are timed. Because the vacuum liquid
discharging begins to work simultaneously when adding liquid to discharge
redundant liquid, the liquid will not spill outside colorimetric cup.
8, SV12 and SV14 valves are responsible for adding cleaning liquid. Before adding
or after the previous adding, open the valve SV12, but SV14 valve is at COM and
NO conduction status (power off status). Because there is a one-way valve in the
3-way top pipeline, cleaning fluid flows into the middle pipeline of the SV12 and
SV14 valves, and time valve SV12, cleaning liquid will remain in pipeline. Open
SV14 valve when adding, cleaning liquid will be added into colorimetric cup through
single way valve under the pressure. About 70ul cleaning liquid is consumed every
time.
Figure 5-7 ISE liquid line

47

Chapter 6 Instrument Hardware Circuit

Hardware configuration
6.1

Figure 6-1 Hardware main frame

PC

SOPC main control board


including the control of AC
and pressure temperature
monitoring

ISE
module
A/D
module
R2
module
R1
module
Sample
module
Reation
module

ISE A/D collection


ISE valve sets
ISE pump sets
A/D
convertion
collection

and

Photoelectricity conversion and


amplification

R2 adding mechanism
R2 reagent disk

R1 adding mechanism
R1 reagent disk

Sampling mechanism
Sample disk

stirring mechanism
Rinsing mechanism
Reaction disk

48

6.2

Security Note:
At working, touching hardware panel with hand or any other objects is forbidden.
In order to dismount panels, operation is only allowed when cut off power (220V, AC).

6.3

Electrical panel list


Electrical panel No. and function list

Figure 6-1 CS400Circuit board list


PCB Name

Description

Electrical
panel No.

1carrying on the communications between upper


and lower machine, and that with cooling board.
Main controller
board

2Solenoid valve controlSV8SV10SV13SV16


SV41
3Monitoring of high and low temperature water

tanks

4 Monitoring of incubation bath and vacuum tank


positions,
5AD board data processing
6Solid-state relay board control
1, communications with the main control board
stirring mechanisms control
Reaction disk
Circuit board

2, two

3, the reaction disk rotation mechanism control


4, rinsing mechanism control

5Solenoid valveSV6SV11SV12SV14SV15
SV21SV30SV31SV42SV43
6Alkaline cleaning liquid level monitoring
1, communication with the main control board
2, reagent 1 probe mechanism control
Reagent 1 disk
Circuit board

3, reagent 1 disk control


4, solenoid valve control: SV2, SV5

49

5, buzzer control

Reagent 2 disk
Circuit board

1, communication with the main control board


2, reagent 2 probe mechanism control
3, reagent 2 disk control
4, solenoid valve control: SV3, SV9

Sample disk
Circuit board

1, communication with the main control board


2, sample probe mechanism control
3,

sample disk control

4, solenoid valve control: SV3, SV9


5, the sample disk rotating lamp control

ISE

1, communication with the main control board

Circuit board

2, ISE pump motor control (internal standard, dilution,


reference)
3, solenoid control: MAGNET1, MAGNET2
4, solenoid valve control: ISV1, ISV2, ISV3, ISV4,
ISV5, ISV6, ISV7, ISV8, ISV11, ISV12, ISV13
5, ISE preamp board data collection

1,200 W Heater Control


Solid relay board

2,450 W Heater Control


3, gear pump control
4, vacuum pump control
5, magnetic pump control
6, water circulation pump
7, halogen control

AD board

12 AD-wavelength data collection

Cooling board

1, semiconductor refrigeration control and temperature


display

50

2, semiconductor current monitoring and current value


display
3, fan control system

Circuit connecting
Board

1, circuit board and the external circuit or device


connection
2, part of the status indication (Indicator)

Level

detecting

board

1, sample probe liquid level detection


2, reagent 1,2 liquid level detection
3, incubation bath liquid level detection
4, alkaline cleaning liquid level detection
5, vacuum liquid level detection

ISE

Preamp

K, Na, Cl electrode three-way preamp

board
Mother board

1, providing reaction disk board, sample disk board,


sample disk, reagent disk, reagent 1,2 disk boards,
main control board, ISE board with connection and
power supply.
2communications among circuit panels

51

6.4

Instrument electrical principle wiring

Figure 6-4-1 Control wiring of communication system

52

Figure 6-4-2 Power switch wiring

Figure 6-4-3 Cooling board wiring

53

Figure 6-4-4 Main control board wiring

54

Figure 6-4-5 Solid relay panel wiring

Solid
board

relay

12V switch
NES-35-12

55

Figure 6-4-6 Circuit panel control wiring of reagent R1 disk

56

Figure 6-4-7 Circuit panel control wiring of reagent R2 disk

57

Figure 6-4-8 Circuit panel control wiring of reaction disk

58

Figure 6-4-9 Circuit panel control wiring of sample disk

59

Figure 6-4-10 ISE panel control wiring

60

6.5

Circuit panel dismounting


When dismounting, pull out the connector on circuit panel first, and then loosen the set
screws to take out the circuit panel from circuit box.

6.6 Circuit function


6.6.1 Control configuration
CS-400 auto-chemistry analyzer structure consists of analysis part (host), operation part
(computer) and the result output part (printer).
Analysis part (host) mainly consists of the temperature control system, the reaction
system (including the ISE module), optical detection system, sample and reagent
processing system, mixing system, liquid line system and the reaction cup cleansing
mechanism.
Overall function of instrument control structure hardware system:

Achieve serial communication with the PC and the completion of command,


response and data transceipt
control data acquisition of optical system;
control the movement and
implementation mechanism;

status signal collection of the movement

control temperature control system as well as temperature control signal


collection;
control ISE electrode data collection;
Control the cooling system.

6.6.2 Main control board function


Main function of main control board

communicate through the serial port with the PC to realize transmission


of data, instruction and warning information
communicate through the motherboard with the reaction disk board, the
sample disk board, reagent 1 disk board , reagent 2 disk board, ISE board, cooling
board, AD board to transmit data and instruction;
monitoring water tank water level and temperature
control water tank temperature

61

6.6.3 Reaction disk/ sample disk / reagent 1 disk / reagent 2 disk/ ISE board function
The main function of circuit boards of three disks are to receive the main control
board's instruction to complete the reaction disk, the sample disk, reagent 1 disk, reagent
2 disk, ISE circuit board work, the specific functions as follows:
Each CPU communicates with the main control board to receive instruction;
Each CPU outputs control order of each executive unit;
to receive the sensor signals and other status of implementation unit;
sent to the main control board If alarm occurs,
6.6.4

AD collecting panel function


AD collecting consists of two parts: AD data preamp board and AD data collecting
board
AD data preamp board
Preamp board circuit realizes the photoelectric conversion function of discrete
photodiode array whose 12 pixels convert the multiwavelength homochromous light
signal after transmitting through reaction cup into electric signal, converted by
preamp circuit and sending the converted voltage signal to AD collecting board to be
processed.
AD data collecting board
AD data collecting board circuit realizes signal adjustment of photoelectric signal
output by preamp board and AD collecting function. Photodiode array whose 12
pixels convert the multiwavelength homochromous light signal after transmitting
through reaction cup into electric signal, and the 12 photoelectric colorimetric
signal output by photoelectric check board is filtrated and amplified by AD collecting
board, sent to the input terminal of AD convertor by multi-choosing switch, and at
the same time, receive the control signal of main control board to sample one by one
from the processed 12 photoelectric signals, and send the AD value of reflecting
light intensity to the main control board to process. Besides, AD data collecting
board is responsible for the power supply of preamp board.

6.6.5 Cooling board function


Cooling system consists of three parts: semiconductor cooling model, radiator, and fan.
The main function of cooling board is to control semiconductor refrigeration module,
keeping the water of cold water tank at 6 degrees -10 degrees, to meet the needs of
reagent refrigerating warehouse refrigeration function, maintaining the temperature of
reagent refrigerated storehouse at the regulated range.
Main function:
control module semiconductor refrigeration
cooling system fan
control

62

6.6.6 Power supply system

List of power supply used by circuit panel



Series

NE series small-size switching power supply

type

NET-50B

Output voltage

DC 5V,0.6-5A;12V0.2-2.5A;-12V,0.1-0.7A

Output wattage

50W

Output set

3sets

Temperature
range
Input voltage

-20+60
85-264VAC/120-370VDC

size

129*98*38mm

Warranty period

2years

manufacturing
location

Guangzhou


Series

NE series small-size switching power supply

type

NES-15-12

Output voltage

DC 12V,0-1.3A

Output wattage

15W

Output set

1set

Temperature range

-20+60

Input voltage

85-264VAC(120-370VDC)

size

79*51*28mm
Warranty
period

manufacturing location

2 years
Guangzhou

63


Series

NE series small-size switching power supply

type

NES-15-5

Output voltage

DC 5V,0-3A

Output wattage

15W

Output set

1set

Temperature range

-20+60

Input voltage

85-264VAC(120-370VDC)

size

79*51*28mm
Warranty
period

manufacturing location

2 years
Guangzhou


Series

PFC series

switching power supply

type

SP-500-24

Output voltage

DC 24V,020A

Output wattage

500W

Output set

1set

Temperature range

0+40

Input voltage

88264VAC

size

170*120*93mm
Warranty
period

manufacturing location

2 years
Guangzhou

64


Series

NE series small-size switching power supply

type

NES-35-12

Output voltage

DC 12V,0-3A

Output wattage

35W

Output set

1set

Temperature range

-20+60

Input voltage

85-264VAC(120-370VDC)

size

99*97*36mm
Warranty
period

manufacturing location

2 years
Guangzhou

65

Chapter 7 Maintenance and Overhaul


In order to ensure reliable system performance, excellent working status and span,
please conduct system operation and regular maintenance strictly in accordance
with the requirements in the repair manual. Learning maintenance and overhaul of
this chapter is also very important and in-depth study will enable the instrument to
achieve the best running status and exert the best performance.
Warning
Do not carry out maintenance this chapter doesnt mention.
Otherwise, it could lead to system damage and personal injury.
Do not touch any other parts except user self-operation and
maintenance which are clear recorded.
Unauthorized repair of the system may lead to system damage
and personal injury, and commitment term of the repair contract is
no longer valid.
Upon completion of maintenance work, make sure the system is
working normally.
Do not splash water, reagent and other liquid onto the system's
mechanical or electrical parts.
Biological contamination danger

In the process of maintenance work, be sure to wear gloves, put


on work clothes to prevent them from being infected and, if
necessary, wear protective glasses.
7.1Maintenace preparation
Tools, intensified cleaning liquid and alcohol maybe used in the process of working.
7.1.1

Tools

1. One set of hexagon wrench


2. Cruciform Screwdriver (large, medium and small)
3. Injection needle hose
4. Small tweezers
5. Clean gauze
7.1.2 Intensified cleaning liquid
1. Acid cleaning agent, 0.1mol / L hydrochloric acid
2. Alkaline cleaning agent, 0.5% (V / V) sodium hypochlorite
Warning
Intensified acidic and alkaline cleaning liquid mixed generate
poisonous gas. Do not mix the intensified acidic and intensified
alkaline cleaning liquid.
Caution
Following cleaning liquid designated by Dirui Co., Ltd.
Intensified acidic cleaning liquid: 0.1mol / l hydrochloric acid;
Intensified alkaline cleaning liquid: 0.5% (V / V) sodium
hypochlorite.
Please use the intensified cleaning liquid designated by Dirui. If
outside of designated types of intensified cleaning liquid are used, it
may not be able to receive appropriate the results of the analysis.
Dirui recommends the use of alternating acidic and alkaline
cleaning liquid, for example, use intensified acidic cleaning fluid
after power on, then use of intensified alkaline cleaning liquid next
time after power on.

66

7.2 Daily maintenance item


7.2.1 Check injection pump
The purpose of checking the injection pump is check whether the leakage exists.
1, Make sure that Power of analysis part has been switched off.
2 open the front door and it is shown as Figure 6-1
If with ISE system, the left are the three ISE unit injection pumps, the middle are
injection pumps of sample, reagent 2, reagent 1 respectively.

Figure 6-1
3. To observe whether the injection pump is leaking,
If so, check the leakage causes, and check the pipeline and connector timely.

7.2.2 Check/rinse sample, reagent probe


1In online status, click Instrument resetting in Maintenance, and instrument
executes resetting.
2When cleaning ample and reagent probes, carefully observe whether the
outflow of sample probe internal wall is continuous, whether the direction of flow is
consistent with the sample probe and the outflow of external wall is continuous, ,
and whether water volume is normal.
If not normal, clean sample probe
If still not normal, check the corresponding liquid line channel; check whether
water supply of water tank and water pressure is normal.
7.2.3 Rinsing stirring rod
1In online status, click Instrument resetting in Maintenance, and instrument
executes resetting.
2, when cleaning, carefully observe whether the stirring rod works normally, If not
normal, check the corresponding liquid line channel, check whether water
supply of water tank and water pressure is normal.

67

7.2.4 Rinse rinsing mechanism


1In online status, click Instrument resetting in Maintenance, and instrument
executes resetting.
2When rinsing, carefully observe rinsing probe working and whether probe
infusing is normal and assimilating is completely.
If infusing abnormal, check pressure value of water infusing pressure gauge
If assimilating abnormal, check pressure value of assimilating vacuum

7.2.5 Check waste connection and discharging

Biological contamination danger:


During waste water operation, please put on gloves, put
on work clothes and if necessary, wear protective glasses.
Check whether liquid waste disposal system is normal every day, and maintain
waste liquid pipe is not bent and discharges smoothly and high and low
concentration waste liquid are disposed properly (refer to local standards of dispose
waste liquid).

Caution:
Make sure liquid flow catheter was not bent and flows smoothly.
Otherwise, it may result in that poor waste water spills from the cover
panel of analysis part, even the serious damage of analysis part.

7.2.6 Rinse instrument surface


Rinse instrument surface daily to keep the clearness of instrument surface.
7.2.7 Check printer and printing paper
Check printer power supply indicator, preparation indicator and printing paper daily.

68

7.3 weekly maintenance items

7.3.1 Rinse sample probe/reagent 1/ reagent 2 probe

Warning:
Please be careful to avoid hands from being scratched
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean sample probe at your own will,
please follow the relevant provisions for proper disposal.

Make sure the analysis part power supply is switched off.

Lift the rotating arms of sample and reagent probes by hands to the top
position, and rotate them to the top of sample or reagent storehouse for
convenient operation.

Caution:
When cleaning, do not touch directly the sample
surface to prevent probe scratch; avoid too much hand
force to prevent deformation of the sample probe.

69

Note:
Acidic and alkaline cleaning liquid can be used
alternatively, for instance, acidic cleaning liquid is used
at previous time maintenance, use alkaline cleaning
liquid at this time maintenance.

Wipe the external walls of sample and reagent probes lightly with cotton
stick moisturized with alcohol, especially the point of probe, until no
impurities left at all.

Wipe sample and reagent probes with the gauze dipped with deionized
water

After cleaning, lift the rotating arms of sample and reagent probes to the top
position, and rotate the rotating arm of sample probe to locate the sample
probe above the rinsing bath of sample and reagent probes.
Caution:
After cleaning the surface of a sample probe, please
make sure sample probe must be rotated to the top of
sample probe rinsing bath.

Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.

70

7.3.2 Rinse stirring rod


Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean sample probe at your own will,
please follow the relevant provisions for proper disposal.

Make sure the analysis part power supply is switched off.

Lift the stirring rod by hands to the its top position, and rotate its rotating
arm to the above a position for convenient operation.

Caution:
When cleaning, do not touch the sample surface directly to
prevent probe scratch; avoid too much hand force to prevent
deformation of the sample probe.
Note:
Acidic and alkaline cleaning liquid can be used alternatively,
for instance, acidic cleaning liquid is used at previous time
maintenance, use alkaline cleaning liquid at this time
maintenance.

Wipe the surface of stirring rod lightly with cotton stick moisturized with
alcohol, especially the point of probe, until no impurities left at all.

Wipe stirring rod with the gauze dipped with deionized water

After cleaning, lift the rotating arms stirring rod to the top position, and
rotate the rotating arm of stirring rod to locate the stirring rod to the top of
the rinsing bath of stirring rod.

Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.

71

7.3.3 Rinse sample/reagent barcode window

Caution:
Do not gaze scanning laser light, or it may cause eyes injury

Make sure the analysis part power is switched off.

Remove the reagent and sample disk covers, and then remove the sample
and reagent disks.

Wipe the scanning glass window lightly with gauze dipped with deionized
water.

Remount the sample and reagent disks and cover them.

Switch on the analysis part and wait 30 seconds, the system will reset
automatically.

7.3.4 Rinse sample storehouse


Warning:
Please be careful to avoid being scratched by sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean sample probe at your own will,
please follow the relevant provisions for proper disposal.
1

Make sure the analysis part power is switched off.

Remove the sample diskfigure

Rinse sample disk with water and wipe it with gauze

Wipe sample storehouse internally with clear gauze. If necessary, wipe it


with gauze dipped with a little pure water or disinfector.

Mount sample disk, tighten disk fixing screws clockwise and cover it.

72

7.3.5 Rinse reagent refrigeration storehouse

Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.

Make sure the analysis part power is switched off.

Remove reagent disk cover and reagent disk

Rinse sample disk with water and wipe it with gauze

Wipe sample storehouse internally with clear gauze. If necessary, wipe it


with gauze dipped with a little pure water or disinfector.

Mount sample disk, tighten disk fixing screws clockwise and cover it.

7.3.6 Rinse reaction cup


The contamination of sample probe, reagent probe, stirring rod and reaction cup will
affect the accuracy of measurement. The reaction cup is required intensive cleaning.

Place 70 ml detergent (1mol of hydrochloric acid or 0.5% NaOH solution,


use the two types of solution alternatively, one week one solution) at the
45th detergent position of reagent disk.

Click Maintenance functional key to enter System maintenance menu,


and select Rinse reaction cup to execute.

73

7.4 Monthly maintenance item

7.4.1 Rinse sample,

reagent 1, reagent 2 probe rinsing bath.

Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.

Make sure the analysis part power is switched off.

Lift the rotating arm of sample probe by hands to the its top position, and
rotate its rotating arm to keep sample probe away from rinsing bath for
convenient operation.

Clean the inside and appearance of sample probe rinsing bath with clean
cotton stick.

After cleaning, lift the rotating arm of sample probe to the top position, and
rotate the rotating arm of stirring rod to locate the sample probe to the top
of the rinsing bath of sample probe.

Caution
After the work of sample probe surface rinsing, please
make sure to rotate the sample probe to the top of
sample probe rinsing bath.
5

Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.

74

7.4.2 Rinse stirring rod rinsing bath

Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.

Make sure the analysis part power is switched off.

Lift the stirring arm to the top position by hand to one side of rinsing bath.

Wipe stirring rod with clean soft gauze.

7.5 Every 46 months maintenance item


7.5.1 Dust-proof disposal of cooling unit
1

Make sure the analysis part power is switched off.

Open right back cover board

Remove the three screws of cooling unit and remove the cooling unit.

Remove the dustproof from the cooling unit and rinse it with water and dry
it.
figure

Remount dustproof net cleaned already.

Mount back cover board

75

7.6 Every half a year maintenance item

7.6.1 Check light source lamp


Lamp light source of optical system will gradually be aged in use, and will cause an
increase in noise measurements. If the cup blank and light source intensity
attenuation is out of range or the working time of light source lamp accumulates over
2000 hours, the light source lamp should be checked.

Caution
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.
Do not touch the light source lamp shell surface and lens in front of the
light source lamp by hand, because it may change the characteristics
of the light source. If you accidentally make noodle stained with filth,
absorbent cotton dipped by absolute alcohol can be used to clean it.

Turn off the system main power, so that the light source box and light
source lamp will be cooled for at least 15 minutes.
Warning:
High-temperature light source lamp and light box will
cause burn. Operation is carried out only after the light
source and light source lamp are cooled.

Loosen the fixing screws of rinsing mechanism; remove the rinsing


mechanism of reaction cup. Loosen the setscrews of reaction disk and
remove the reaction disk. Place the reaction cup at dry and clean position.
Loosen the two fixing wire connecting poles of halogen and remove
down-lead.

76

Loosen the two screws fixing light source seat to remove halogen lamp.

Mount a new halogen lamp according to the above opposite steps; pay
attention to tighten the screws. The cooling rubber hose in the lamp room
can not be twisted and down-lead can not be loosed or cocked.

Remount the reaction disk, the reaction cup and rinsing mechanism;
switch on the power supply of analysis part. After standby mode,
single-click Next in System maintenance window; infuse purified water
into reaction groove. After instrument standby mode, execute light quantity
check function. Check the back of halogen lamp if the light quantity
conforms to the requirement to start test.

7.6.2 Check all air filter net


After long-term use, the ventilation performance of air filter, which is not good,
needs to be checked.

7.6.3 Check and clean container and floater switches.


7.6.4 Check water liquid pipe
After long-term use, if any unsmooth discharging occurs, the waste liquid pipe of
designated specification can be used to check former waste liquid pipe.
7.7 Every 1 year maintenance item

7.7.1 Check water of cooling system

77

7.8 Irregular check item

7.8.1 Rinse sample probe and reagent probe


If the water flow is not normal when cleaning sample probe, sample probe and reagent
probe may have been blocked and cleaning is needed to sample probe.
Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.
1

Make sure the analysis part power is switched off.

Remove sample disk cover and then sample disk

Lift the rotating arm of sample probe by hands to its top position, and rotate
its rotating arm to keep sample probe away from rinsing bath for
convenient operation.

Hold shell claw of probe rotating arm with fingers and lift to remove.

Loosen pipeline interface

78

warning
Carefully place dismounted sample probe and prevent it
scratching human body and sample probe damage.
Note
Take out sample probe from the rotating arm and be careful
to operate to avoid the damage of probe point caused by
touching rotating arm.

Note
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision. Please
refer to "Error! Reference source not found. Error! Reference source
not found." for specific check of sample probe.
7.8.2 Clean sample probe/reagent probe
Warning
Please be careful to avoid being scratched by sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean sample probe at
your own will, please follow the relevant provisions for
proper disposal.

impurity in the probe.

Caution
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision.
Please refer to "Error! Reference source not found. Error! Reference
source not found." for specific check of sample probe.

79

7.8.3 Install sample/reagent probe


Warning
Please be careful to avoid being scratched by sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best
Dismounting sequence is opposite to that of sample probe/reagent probe.
Caution
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision.
Please refer to "Error! Reference source not found. Error! Reference
source not found." for specific check of sample probe.
7.8.4 Rinse sample probe/reagent
When it is found that the water level of rinsing bath is too high when rinsing sample
probe because of no discharging available, which may be caused by the blocked
leaking hole. Cleaning sample rinsing bath is necessary.
Warning
Please be careful to avoid being scratched by sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best
1

Make sure the analysis part power is switched off.

Lift the rotating arm of sample probe by hands to its top position, and rotate
its rotating arm to keep sample probe away from rinsing bath for
convenient operation.
Infuse about 1ml alkaline detergent of 0.5% (V / V) sodium hypochlorite or
84 disinfectant into rinsing bath for 10 minutes.

3
4

Switch on the power supply of analysis part

Lift the rotating arm of sample probe by hands to its top position, and rotate
its rotating arm to keep sample probe above the rinsing bath of sample
probe.
Caution
Please rotate the sample probe to the top of sample probe
rinsing bath after clean rinsing bath of sample probe.

Select and execute Instrument resetting after enter Maintenance-routine


maintenance, and the system will reset sample probe and sample probe
and rinsing bath will be rinsed with deionized water automatically. Observe
the outflow of sample probe rinsing bath.

80

7.8.5 Rinse stirring rod


If the stirring rod is damaged, please check stirring rod in accordance with following
steps strictly.

Warning
Please be careful to avoid being scratched by sample probe.
Any touch is forbidden except at the knurling of it only by hand when
checking, and prevent any scratch on the flat part of stirring part.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best
Please deal with removed stirring rod properly.
Caution
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.

Make sure the analysis part power is switched off.

Prepare a new stirring rod and wipe the flat part of it with gauze or cotton
stick dipped with cleaning liquid, and then wipe it with gauze dipped with
deionized water.

Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm for convenient operation.

81

Caution
When pulling out stirring rod, make sure the direction of
force is vertical to that of axis of rotating arm. Lateral force
may damage the axis or stirring rod.
4

Remove stirring rod after loosen the two fixing screws.

Prepare a new stirring rod, and wipe the front of the stirring rod with gauze
dipped with 2% CS-antibacterial cleaning liquid.

When mounting new stirring rod, insert stirring rod till the bottom of motor
axis and tighten it with M2 screw.
Caution
When inserting stirring rod, make sure the direction of force is
vertical to that of axis of rotating arm. Lateral force may
damage the axis or stirring rod.
Pushing the stirring rod completely

After stirring rod check, visually check whether stirring rod and its rotating
arm are vertical with each other.
If not vertical, return to step 5 and remount the stirring rod.
If vertical, continue to next.

Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm to the top of its rinsing bath.
Caution
Please make sure to rotate stirring rod to its rinsing bath top
after mount it.

Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement instrument
resetting", the system will automatically reset the sample probe and rinse it
with deionized water. Observe the outflow of sample probe.

82

7.8.6 Check reaction cup

Warning
Please be careful to avoid being scratched by sample probe.
Place each probe and pole into proper position for convenient.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best
Please deal with removed reaction cup properly which is broken.
Caution
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.

Switch

off

instrument

2 Put on protective gloves to remove fixing screws.

83

Rotate reaction disk by hand and remove the reaction cup sequently. Take out
reaction cup while rotating it.

Rinse the new reaction cup dipped in with water; rinse inside and outside of
reaction and no scratch is allowed.

Rotate reaction disk by hands, and mount new reaction cup on reaction disk
and check the six sets reaction cups simultaneously.

Mount reaction disk with the opposite steps and make sure the fixing screw of
reaction disk is tight.

Switch on the power of analysis part


Select and execute cup blank test after click System maintenance, and
observe execution result and reaction status.

84

Chapter 8 Assay Method

CS-400 adopting three analytical methods as follows:


point assay
two-point assay
rate assay
8.1

Analysis principle

The assay mode of Auto-Chemistry Analyzer is based on the Beer-Lambert law that the
material selective absorption light
The main principle is: When monochromatic light with specific wavelength passes
through the cuvette with sample, the monochromatic light absorbency and sample liquid
concentration are varies directly as the distance which is passed through sample liquid by
light:

I0
= b c
It
A Absorbency of the light when passing through liquid
A = lg 1/T = lg

T Transmitted intensity and incident intensity ratio: transmittance It/I0


I0 Incident intensity
It Transmitted intensity
Molar absorption coefficient of solutionmlmmol 1cm 1

c Mol concentration of the solutionmmol/ml

b Solution layer thicknesscm

Solution layer thickness (b): Optical path, which is fixed by instrument. Molar absorption
coefficient () is the correlation coefficient of the wavelength, solution and solution
temperature. Linear relationship is displayed between solution thickness and absorbency
when in stable temperature and single wavelength value is given on the reagent bottle by
factory
If the sample liquid adequate distribution, interaction between liquid and incidence
monochromatic light only happens during absorbing process. No fluorescence, disperse and
photochemical appear. No interaction between substances in the solution while absorbing
process. The absorbency possess conducts nature, and this condition conforms to the
Beer-Lambert law

85

8.2

Assay mode variety

Method

Photometry point
L 0 0 0

1-point
Assay

AL + AL 1
2

1LM49

B1 + B 2 + B 3
3

( AM + AM 1 ) k ( AL + AL 1 )
2

L M 0 0

B1 + B 2 + B 3
3

AM + AM 1 AL + AL 1

2
2
t

L M 0- 0

2-point
Rate Assay

Formula

B1 + B 2 + B 3
3

1L49

2-point
assay

Cell
blank

Remark

L
M

1LM49
L M 0 - 0
Rate
Assay

1LM49

B1 + B 2 + B 3
3

AM-L

L +2M
Fist half
1-point
&
Rate Assay

L 0 0 0
1 M NL P
Q49

B1 + B 2 + B 3
3

AL + AL 1
2

86

Second half
MNPQ
1 M NL P
Q49

B1 + B 2 + B 3
3

AQ- P-kAN -M

M+2N P+2Q
Fist half
L 0 0 0
3-point dual
item

B1 + B 2 + B 3
3

AL + AL 1
2

B1 + B 2 + B 3
3

( AN + AN 1 ) k ( AM + AM 1 )
2

1LMN49
Second half
MN00
1LMN49
Fist half
L M 0 - 0
3LMNP49

Rate
B
Assay
(mode 1 )

B1 + B 2 + B 3
3

AM-L

L +2M

An p
wavelength

Second half
NP00
3LMNP49

B1 + B 2 + B 3
3

N+2P

diffe

from the first Half

Two conditions

AnpkALmsame
wavelength as the second
half

Fist half
Rate
B
Assay
(mode 2 )

L M 0 0
3LMNPQ
R49

B1 + B 2 + B 3
3

AM-L

B1 + B 2 + B 3
3

A(R-Q)kA(P-N)

L +2M
Second half
N P Q R
3LMNPQ
R49
N+2P Q+2R

87

L,m,n,p,q,r

: Photometric points

Rn

Volume of nth reagent ,n=1 to 4

B1B 2B 3

Stopped cell blank

(B1,B2,B3 )/3

Passed cell blanks

Ax

Absorbance at photometric point x


Change in absorbance per minute between photometric

A(m-L)
points L and M

Liquid volume correction factor

k
a

k=

S + Rj
j =1
b

S + Ri
i =1

Sample volume
Rj Ri

a: No. of reagents with correction (at Al measurement)

b: No. of reagents without correction(at Am measurement)

Note 1: The 5 th Photometric point wont be Stirred after adding reagent 2. Stirring
when the reaction disk pauses after rotates one circle plus 2 pitches
Note 2: liquid in the reaction cuvette should be more than or equal to 150 ul, less
than or equal to 450ul.
Note 3: Do input 0 if the photometric point is not used.

88

8.3

Endpoint assay

Endpoint assay means the reaction takes a period time. Due to reaction balance constants
are big, all substrates (tested) are transformed into product when reaction reaches balance,
and no increase (decrease) of reaction solution absorbance will occur, and the degree of
absorbance increase (decrease) and the concentration of tested substance is directly
proportional. This assay is call endpoint assay or balance assay more accurately, which is
the ideal assay mode.
The endpoint assay is not sensitive to small changes (such as enzyme amount, pH,
temperature, etc.) as long as this change does not affect the balance in certain time.

A
b

figure 8-3 endpoint assay reaction curve

Cell blank

Time

89

Example 1TBILTotal bilirubin reagent kit


wavelength
test mode
reagent
Single
reagent
temperature
reaction
sensitivity
calibrator
Reference
value

Absorbance 02A
range
endpoint assay
Optical path 10mm
sample
10uL
1250uL
Mixing
reagent A:B50:1
storage
37
incubation 10min
10min
0
550nmblank pipe
5mA equals to 1umol/L
Linearity
300umol/L18mg/dL
range
Unit
1umol/L0.0585mg/dL
89.6umol/L0.425A
conversion
Adult5.119umol/L0.31.1mg/dL
Baby20200umol/L1.212mg/dL
Main 550nmsub 660nm

Example 2UAuric acidUric acid liquid reagent kit


wavelength
Test mode
Reagent
Single
reagent
temperature
reaction
sensitivity
calibration
Reference
value

Absorbance
02A
range
Optical path
10mm
sample
4uL
Mixing
285days stable
storage
3730, 25
incubation
5min6min , 8min
5min
0
520nmblank pipe
0.42mA equals to1umol/L
Linearity
1.5mmol/L25mg/dL
range
0.72mmol/L0.302A
Unit
1mmol/L16.8mg/dL
conversion

child0.120.33mmol/L2.05.5mg/dL

Male0.210.43mmol/L3.57.2mg/dL

female0.150.36mmol/L2.56.0mg/dL
urine 14.944.6mmol/L250750mg/dL

Main 520nm 500,550


optional
Endpoint assay
1200uL250uL
4 reagent 1and 1reagent 2

90

8.4

2-point assay

2-point assay (fixed time assay) is also called first class dynamics assay, which means the
reaction speed is in direct proportion to the simple power of substrate concentration in
specified time, namely v=k[S]. Due to the reduction of substrate, the whole reaction speed
is decreasing gradually, which reflects the decrease (increase) of absorbance and
decrease of speed. Because reaction time to reach balance is very long, , it can be
monitored at any time theoretically, but because of the complexity of serum ingredient and
much reaction, therefore, it takes a certain period of time to enter in stable reaction phase.

Figure 8-2

2-point assay reaction curve


Absorbance

Absorbance

Reaction limit level

Cell blank

Time

Time

2-point assay
8.5

2-point rate assay

Rate assay

Rate assay, also known as zero-class dynamics assay, refers to the reaction rate is directly
proportional to the zero power of substrate concentration, which has nothing to do with the
substrate concentration. Hence, the reactants can generate a certain product at constant
speed throughout the reaction process, resulting in even decrease or increase of
absorbance of measured solution at a wavelength, and the decrease or increase speed (
A / min) is directly proportional to the activity or concentration of the tested substance
(catalytic material). Dynamics assay is also called as the continuous monitoring assay,
mainly used for the measurement of enzyme activity.
In fact, because substrate concentration can not be big enough, with the reaction
proceeding, the reaction is no longer zero class when substrate is consumed to a certain
extent, Therefore, zero-class dynamics assay is targeted at a specific period in terms of
time; Because reaction time to reach balance is very long, , it can be monitored at any time
theoretically, but because of the complexity of serum ingredient and much reaction,
therefore, it takes a certain period of time to enter in stable reaction phase. So all reagent
manufactures have strict requirements to the two periods
Dynamics assay is based on the changes between specified photometric points to obtain
the absorbance concentration or activity value.
Metering point in accordance with the input form, dynamics method can be divided into
single-band and dual-band dynamics assay.

91

Figure 8-3 Rate assay reaction curve

Absorbance
Time
Example 3ALT/GPT Alanine aminotransferase IFCC

wavelength

Main 340nm

Test mode
Reagent
Single
reagent
temperature
reaction

Rate assay
1200uL250uL
4 reagent 1and 1reagent 2

sensitivity
calibration
Reference
value

3730, 25
60s delaymeasure 60
120s
0.30mA/min equals to
1.0U/L

Absorbance
range
Optical path
sample
Mixing
storage
incubation
0

02A
10mm
15uL
285days stable
5min
340nmblank pipe

Linearity
450U/L7.5ukat/L
range
Unit
1U/L16.6710-3ukat/L
conversion
Female<31U/L<0.52ukat/L
37Male<40U/L<0.67ukat/L

Calculating method


A/min * TV *1000
ALTU/L
6.22 * SV * P
TVThe total reaction volume (mL
SVsample volumemL
Poptical path of colorimetric cupcm
6.22NADH position mmol extinction coefficient at 340nm 334nm6.18365nm3.40

92

8.6

Principle of electrolyte measurement

Principle
Internal standard solution is firstly added in diluting trough by instrument, and assimilates it
and discharges it into the Na, K, and Cl electrode solution line through the SIP injection
pump to measure its electrode potential which is relative to reference electrode potential.
At this time, SIP injection pump first assimilates reference electrode solution and
discharges it into the reference electrode solution line, and then switch to liquid line to
assimilate the internal standard. And redundant internal solution is assimilated by vacuum
nozzle to vacate diluting trough.
Diluent will be mixed after the sample probe assimilated sample and discharged it into
diluting trough. Diluted sample is as same as the internal standard solution and will be
taken out by SIP syringe pump and the internal standard solution is add into diluting trough
to rinse it. Used for cleaning, the internal standard solution will be pumped through the SIP
pump. After vacate diluting trough, assimilate the internal standard solution for the next
round of testing.

93

Figure 8-4 ISE Work flow

Infuse sample

Assimilate internal solution to


rinse diluent bath

Measure sample concentration

Assimilate diluted sample from


diluent bath

Diluting sample

Discharge
solution

internal

standard

Measure and calibrate the concentration of


internal standard solution

Assimilate reference liquid

Assimilate
internal
standard
solution from diluent bath

Infuse internal standard solution


into diluent bath

94

Principle of generating electrode potential

8.6.1

Electrode potential is obtained by Nernst's formula.

E = E 0 + 2.303

RT
l og(ai )
nF
1

ai = f Ci 2

E0

: The standard potential of the measured system

R: gas constant (8.314510 J mol-1 K-1)


T: absolute temperature (t +273.15) (K)
F: Faraday constant (9.6485309 104 C mol-1)
: Ion (i) activity
f: activity coefficient
Ci: concentration
n: a given ion (i) the charge number (Cation is positive, anion is negative)
8.6.2

Test method

Working curve preparation, the internal standard solution concentration measurement,


concentration calculation, and result modification are explained as follow.
8.6.3.1 Working curve preparation
Measure low concentration slope of liquid (S1) and high concentration slope liquid (S2),
and determine slope value (sensitivity) of K, Na, Cl the electrode.

SL =

E ( H ) E ( L)
C(H )
log
C ( L) (3)

SL: slope value (slope)


E (H): the potential of high concentration slope solution
E (L): the potential of low concentration slope solution
C (H): high concentration of the concentration slope solution (input value)
C (L): low concentration of the concentration slope solution (input value)

95

8.6.3.2 The measurement of internal liquid concentration

C ( IS ) = C ( L) 10

E ( IS ) E ( L )
SL

(4)

C (IS): the concentration of internal standard solution


E (IS): the potential of internal standard solution

8.6.3.3 concentration calculation


The calculation of routine sample, STAT sample, and concentration of quality control liquid
is based on the concentration of internal standard solution. Internal standard solution is
different with the different sample.

C ( S ) = C ( IS ) 10

E ( S ) E ( IS )
SL

(5)

C(S)Sample concentration
E(S): Sample potential

8.6.3.4 result modification


Test calibrator (calibrator S3)of serum category after calibration to calculate its
concentration, and the difference between the tested concentration and input value is used
as compensation value to increase or decrease sample quantitative value.

C (VALUE ) = C (C ) C ( X ) (6)
C (VALUE): compensation value (compensation value)
C (C): Concentration input value of the serum calibrator
C (X): Concentration tested value of the serum calibrator

C ' ( S ) = IF {C ( S ) + C (VALUE )}

.. ... ... ... ... ... ... ... ... ... ... ... ... ... (7)

C '(S): modified sample concentration


IF: Instrument constant

(usually 1.0)

96

8.6.3.5 Standard specification of electrolyte

Item
Sample
volume
Diluent volume
Processing
ability
Measuring
range

Reagent
consumption
volume

specification
15ul
450ul
80sample/honly measuring electrolyte
Na + 20 ~ 200mmol / L (when only serum )
10 ~ 400mmol / L (when measuring urine)
K + 1.0 ~ 15.0mmol / L (when only serum)
1 ~ 200mmol / L (when measuring urine)
Cl-20 ~ 200mmol / L (when only serum)
10 ~ 400mmol / L (when measuring urine)
Internal standard solution 1050ul / samples (only for
continuous
determination
of
electrolyte )
Diluent 450 ul / sample
Reference Electrode Solution 130 ul / sample

Note:
Internal standard solution will be added if there is no electrolyte analysis for more than 10
minutes in order to activate the electrode.

97

Chapter 9 Malfunction Analysis

9.1

Stirring mechanism 1

Alarm
Code

Description

Detailed
description

Solution

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status

Malfunction 1stirring mechanism stops


Solution:

1-1

R1stirring
mechanism
abnormal

R1stirring
mechanism
fails to
reach the
top of
rinsing bath
side.

1, Check stirring mechanism up-down movements,


mechanical repair is required if resistance is big.
2, check whether both ends of connector of the motor
are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
Malfunction 2stirring mechanism can reach the top,
but can not check the zero position.
solutionManually make stirring mechanism
repeatedly rise to peak, and observe ud_st1 adapter
indicator status.
1sparkling of ud_st1 indicator means the wiring of
light sensor and adaptor is normal.

98

(1) Check whether the conductivity from adapter to


reaction disk circuit board is good and both ends of
connector are connected well.
(2) Check input part circuit of reaction disk circuit
board light sensor signal.
2no sparkling of ud_st1 indicator, check ud_st1

1check lead
Check whether the conductivity from adapter J273 to
light sensor lead P273-P403789 is good and both
ends of connector are good.

2check light sensor signal


check voltage between P273 plug pin 7,9, if the
voltage is not 5v, see (4); if voltage is 5v, check the
potential of 8-pin socket of the P273. The potential is
high when rose to peak, otherwise the potential is low.
If the potential is normal, check the adapter board; not
normal, check the light sensor

3check voltage between P285 plug pin 46, if the


voltage is not 5v, check adapter board; if voltage is 5v,
check conductivity from adapter board J285 to
reaction disk leadP285-P075is good and both ends
of connector are connected well.
Malfunction 3Stirring mechanism can reach the zero
position.

solutionMechanical repair

1-2

R1 Stirring
mechanism
abnormal

R1stirring
mechanism
fails to
reach the
top of
reaction
side.

R1stirring mechanism fails to reach the topthe


solution is the same as 1-1

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1stirring mechanism stops
solution

1-3

R1 Stirring
mechanism
abnormal

R1tirring
mechanism
can leave
the top
when it
descents

1, Check stirring mechanism up-down movements,

99

mechanical repair is required if resistance is big.


2, check whether both ends of connector of the motor
are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally

Malfunction 2stirring mechanism can reach the top


but not leave
Solution the same to 11 Malfunction 2
In the CS-400 upper instrument software, enter
into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1stirring mechanism stops

Solution
1, Check stirring mechanism up-down movements,
mechanical repair is required if resistance is big.

1-4

R1 Stirring
mechanism
abnormal

R1stirring
mechanism
fails to
reach the
rinsing bath
when it
moves to
rinsing
bath.

2, check whether both ends of connector of the motor


are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
5Check whether the it is at top position, check
whether up-down zero light sensor works normally at
top position.

Malfunction 2 stirring mechanism can sway, but can


not reach rinsing bath.
Solution
1check whether the installation of left and right limit
light sensors is correct.
2in boot-strap status, manually sway stirring
mechanism and observe the right_st1 indicator status

100

of right limit light sensor.


(1sparkling of ud_st1 indicator means the light
sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead P285-P075is good
and both ends of connector are good.

2Check input part circuit of reaction disk circuit board


light sensor signal.
(2if no sparkling of right_st1,check right_st1
1check lead
Check whether the conductivity from adapter J273
to light sensor lead P285-P075pin 456 is good
and both ends of connector are connected well.
2check light sensor signal
check voltage between P273 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P273. The potential is
low when sway to rinsing bath, otherwise the potential
is high. If the potential is normal, indicator remains,
check the adapter board; not normal, check the light
sensor
3check voltage between P285 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is 5v,
check conductivity from adapter board J285 to
reaction disk leadP285-P075is good and both ends
of connector are connected well.

101

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1stirring mechanism stops

Solution
1, Check stirring mechanism up-down movements,
mechanical repair is required if resistance is big.
2, check whether both ends of connector of the motor
are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally

1-5

R1 Stirring
mechanism
abnormal

R1stirring
mechanism
fails to
reach the
reaction
cup when it
moves to
reaction
cup.

5Check whether the it is at top position, check


whether up-down zero light sensor works normally at
top position.
Malfunction 2 Stirring mechanism can realize its
sway movement, but can not reach the reaction cup.
Solution
1check whether the installation of left and right limit
light sensors is correct.
2in boot-strap status,manually sway stirring
mechanismobserve the left_st1 indicator status of
right limit light sensor.

1sparkling of left_st1 indicator means the light


sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead P285-P075is good
and both ends of connector are good.
2Check input part circuit of reaction disk circuit
board light sensor signal.
(2if no sparkling of left_st1,check left_st1
1Check whether the ud_st1 indicator has
malfunction

102

2check lead
Check whether the conductivity from adapter
J273 to light sensor lead P285-P075pin 12
3 is good and both ends of connector are
connected well.
3check light sensor signal
check voltage between P273 plug pin 13, if the
voltage is not 5v, see3; if voltage is 5v, check
the potential of socket pin 2 of the P273. The
potential is low when sway to reaction cup,
otherwise the potential is high. If the potential is
normal, indicator remains, check the adapter
board; not normal, check the light sensor.
4check voltage between P285 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J285 to reaction disk leadP285-P075
is good and both ends of connector are
connected well.

1-6

R1 Stirring
mechanism
abnormal

When
resetting,
R1 Stirring
mechanism
failed to
reach
rinsing
bath.

The solution is the same as 1-4

Enter into system maintenance window and


execute Mechanical movement check. Observe
the running status of stirring rod.
Malfunction 1stirring mechanism stops

1-7

R1 Stirring
mechanism
abnormal

When
resetting,
R1 Stirring
mechanism
failed to
leave
rinsing
bath.

The solution is the same as 1-4


Malfunction 2 Stirring mechanism can realize its
sway movement to rinsing bath, but can not leave it.
The solution is the same as 1-4

1-8

R1 Stirring
mechanism
abnormal

R1 Stirring
mechanism
failed to
reach the
top when
rotating.

If stirring mechanism failed to reach the top when


rotatingthe solution is the same as 1-1

103

9.2
Alarm
Code

Stirring mechanism
Descriptio
n

Detailed
description

Solution

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status

Malfunction 1stirring mechanism stops


Solution:
1, Check stirring mechanism up-down movements,
mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.

2-1

R2 Stirring
mechanis
m
abnormal

4, check the motor drive module of circuit


board is working normally
R2 Stirring
Malfunction 2stirring mechanism can reach the top,
mechanism can
not reach the top
but can not check the zero position.
at rinsing bath
solutionManually make stirring mechanism
side.
repeatedly rise to peak, and observe ud_ st2 adapter
indicator status.
1sparkling of ud_ st2 indicator means the wiring of
light sensor and adaptor is normal.
(1) Check whether the conductivity from adapter to
reaction disk circuit board is good and both ends of
connector are connected well.
(2) Check input part circuit of reaction disk
circuit board light sensor signal
2no sparkling of ud_ st2indicator, check ud_ st2

1check lead
Check whether the conductivity from adapter J272 to
light sensor lead P272-P402789 is good and
both ends of connector are good.

104

2check light sensor signal


check voltage between P272 plug pin 7,9, if the
voltage is not 5v, see (3); if voltage is 5v, check the
potential of socket pin 8 of the P272. The potential is
high when rose to peak, otherwise the potential is
low. If the potential is normal, check the adapter
board; not normal, check the light sensor

3check voltage between P285 plug pin 46, if the


voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J285 to
reaction disk leadP285-P075is good and both ends
of connector are connected well.
Malfunction 3Stirring mechanism can reach the
zero position.
solutionMechanical repair

2-2

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism can
not reach the top
at reaction cup
side.

R2 stirring mechanism fails to reach the topthe


solution is the same as 2-1

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1stirring mechanism stops
solution

2-3

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism can
not leave the top
when
descending.

1, Check stirring mechanism up-down movements,


mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
Malfunction 2stirring mechanism can reach the top
but not leave
Solution the same to 21 Malfunction 2

105

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1stirring mechanism stops

Solution
1, Check stirring mechanism up-down movements,
mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
5Check whether the it is at top position, check
whether up-down zero light sensor works normally at
top position.

2-4

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism can
not reach the
when moving to
rinsing bath
side.

Malfunction 2 stirring mechanism can sway, but


can not reach rinsing bath.
Solution
1check whether the installation of left and right limit
light sensors is correct.
2in boot-strap status, manually sway stirring
mechanism and observe the right_ st2indicator
status of right limit light sensor.
(1sparkling of ud_ st2 indicator means the light
sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead P285-P075is
good and both ends of connector are good.
2Check input part circuit of reaction disk circuit
board light sensor signal.
(2if no sparkling of right_ st2,check right_ st2
1check lead
Check whether the conductivity from adapter J272

106

to light sensor leadP272-P402pin 456 is good


and both ends of connector are connected well.
2check light sensor signal
check voltage between P272 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P272. The potential is
low when sway to rinsing bath, otherwise the
potential is high. If the potential is normal, indicator
remains, check the adapter board; not normal, check
the light sensor
3check voltage between P285 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J285 to reaction disk leadP285-P075
is good and both ends of connector are
connected well.

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1stirring mechanism stops

Solution
1, Check stirring mechanism up-down movements,
mechanical repair is required if resistance is big.

2-5

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism can
not reach the
reaction cup
when moving to
reaction cup
side.

2, check whether both ends of connector of the


motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
5Check whether the it is at top position, check
whether up-down zero light sensor works normally at
top position.
Malfunction 2 Stirring mechanism can realize its
sway movement, but can not reach the reaction cup.
Solution
1check whether the installation of left and right limit
light sensors is correct.

107

2in boot-strap status,manually sway stirring


mechanismobserve the left_ st2indicator status of
right limit light sensor.
1sparkling of left_ st2 indicator means the light
sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead P285-P075is
good and both ends of connector are good.
2Check input part circuit of reaction disk circuit
board light sensor signal.
(2if no sparkling of left_ st2,check left_ st2
1Check whether the ud_ st2 indicator has
malfunction
2check lead
Check whether the conductivity from adapter
J273 to light sensor lead P285-P075pin 1
23 is good and both ends of connector are
connected well.
3check light sensor signal
check voltage between P272 plug pin 13, if
the voltage is not 5v, see3; if voltage is 5v,
check the potential of socket pin 2 of the P272.
The potential is low when sway to reaction cup,
otherwise the potential is high. If the potential
is normal, indicator remains, check the adapter
board; not normal, check the light sensor.
4 check voltage between P285 plug pin 46,
if the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J285 to reaction disk leadP285-P075
is good and both ends of connector are
connected well.

2-6

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism can
not reach
rinsing bath
when resetting.

The solution is the same as

2-4

Enter into system maintenance window and


execute Mechanical movement check.
Observe the running status of stirring rod.
Malfunction 1stirring mechanism stops

2-7

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism can
not leave rinsing
bath when
resetting.

The solution is the same as 2-4


Malfunction 2 Stirring mechanism can realize its
sway movement to rinsing bath, but can not leave it.
The solution is the same as 2-4

2-8

R2 Stirring
mechanis
m
abnormal

R2 Stirring
mechanism fails
to rotate at top.

If stirring mechanism failed to reach the top


when rotatingthe solution is the same as 2-1

108

9.3
Alarm
Code

Rinsing mechanism of reaction cup


Descriptio
n

Detailed
description

Solution
Enter into system maintenance window and
execute Mechanical movement check.
Observe the running status of stirring rod.
Malfunction 1stirring mechanism stops

Solution:
1, Check stirring mechanism sway movements,
mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
Malfunction 2stirring mechanism can reach the
top, but can not check the zero position.

3-1

Rinsing
mechanis
m of
reaction
cup
abnormal

Rinsing
mechanism of
reaction cup fails
to reach the top

solutionManually make stirring mechanism


repeatedly rise to peak, and observe ud_wash
adapter indicator status.
1sparkling of ud_wash indicator means the wiring
of light sensor and adaptor is normal.
(1) check lead
Check whether the conductivity from adapter J285
to light sensor leadP285-P075 is good and both
ends of connector are connected well.
(2) Check input part circuit of reaction disk circuit
board light sensor signal.
(2if no sparkling of left_ wash, check left_ wash
1check lead
2check light sensor signal
check voltage between P271 plug pin 13, if the
voltage is not 5v, see3; if voltage is 5v, check
the potential of socket pin 2 of the P273. The
potential is low when sway to reaction cup,
otherwise the potential is high. If the potential is

109

normal, indicator remains, check the adapter


board; not normal, check the light sensor.

3check voltage between P285 plug pin 4


6, if the voltage is not 5v, check adapter board;
if voltage is 5v, check conductivity from adapter
board J285 to reaction disk leadP285-P075
is good and both ends of connector are
connected well.
Malfunction 3Stirring mechanism can reach the
zero position.
solutionMechanical repair
Enter into system maintenance window and
execute Mechanical movement check.
Observe the running status of stirring rod.
Malfunction 1stirring mechanism stops

Solution:

3-2

Rinsing
mechanis
m of
reaction
cup
abnormal

Rinsing
mechanism of
reaction cup fails
to leave the top
when
descending.

1, Check stirring mechanism up-down movements,


mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of circuit board is
working normally
Malfunction 2Rinsing mechanism of reaction cup
fails to leave the top
solutionthe same to that of malfunction 2 in 3-1

110

9.4
Alarm
Code

Reaction disk
Descriptio
n

Detailed
description

Solution
MalfunctionThe quantity counted by reaction cup
light sensor does not conform to real quantity of cup
rotated.

4-1

Reaction
disk
abnormal

Reaction disk
fails to rotate to
the designated
position.

solutionCheck whether the signal status of counting


light sensor is normal.

1check whether the conductivity from counting


light sensor 1 to lead wire is good.
2check whether the counting light sensor 1 is ok.
3Check input part circuit of reaction disk circuit
board light sensor signal.

Reaction
disk
abnormal

Reaction disk
fails to stop at
the designated
position

MalfunctionReaction disk fails to stop at the


position matching with light sensor.
solutionthe same as 41
MalfunctionReaction disk fails to stop at the zero
position after resetting or the light sensor of zero
position did not check resetting point.

4-3

Reaction
disk
abnormal

Reaction disk
fails to stop at
the zero position
when resetting.

solutionCheck whether the signal status of counting


light sensor is normal.

1check whether the conductivity from zero light


sensor to lead wire is good and the both ends of
connector are connected well.
2check whether the zero light sensor 1 is ok
3Check input part circuit of reaction disk circuit
board light sensor signal.

46

cleaning
liquid level
low

Liquid level of
alkaline
cleaning liquid
kit low.

Add cleaning liquid into cleaning liquid kit.

111

9.5
Alarm
Code

Sample probe
Description

Detailed
description

Solution

Enter into system maintenance window and


execute Mechanical movement check.
Observe the running status of probe arm.

Malfunction 1probe arm stops


Solution
1, move probe mechanism, and mechanical repair is
required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.

5-1

Sample probe
abnormal.

4, check the circuit board of sample is working


normally
Sample
probe fails to
reach the top
(except
Malfunction 2Probe arm can rise to zero position.
reaction cup
side) after it
SolutionManually make sample probe mechanism
rose.
repeatedly rise to peak, and observe ud_s adapter
indicator status.
1sparkling of ud_s indicator means the wiring of
light sensor and adaptor are normal.

1check lead
Check whether the conductivity from adapter J282 to
sample circuit board lead P282-P037 is good and
both ends of connector are good.
(2) check sample circuit board
2no sparkling of ud_s indicator, check ud_s

1check lead
Check whether the conductivity from adapter J281 to
circuit board lead P281-P411 is good and both

112

ends of connector are good.

2check light sensor signal


check voltage between P281plug pin 46, if the
voltage is not 5v, see (4); if voltage is 5v, check the
potential of socket pin 5 of the P281. The potential is
high when rose to peak, otherwise the potential is
low. If the potential is normal, check the adapter
board; not normal, check the light sensor

3check voltage between P282 plug pin 46, if the


voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J282 to
reaction disk leadP282-P037is good and both ends
of connector are connected well.
Malfunction 3probe arm mechanism can not rise
to the zero position.
solutionMechanical repair

Enter into system maintenance window and


execute Mechanical movement check.
Observe the running status of probe arm.

Malfunction 1probe arm stops


Solution
1, manually move probe mechanism, and mechanical
repair is required if resistance is big.

5-5

Sample probe
abnormal.

Sample
probe fails to
leave the
top(sample
side) when it
descending.

2, check whether the flexible cable of liquid level


detecting board is connected well.
3, check whether both ends of connector of the motor
are connected well.
4, check whether the conductivity of motor lead wire
is good.
5 ,check sample circuit board
6, check whether liquid level detector has
malfunction
Malfunction 2 probe arm leaves zero position
SolutionManually make sample probe mechanism
repeatedly rise to peak, and observe ud_s adapter
indicator status.

113

1sparkling of ud_s indicator means the wiring of


light sensor , lead and adaptor is normal.

1check lead
Check whether the conductivity from adapter J282 to
sample circuit board lead P282-P037 is good and
both ends of connector are good.
(2) check sample circuit board
2. if no sparkling of ud_s indicator, check ud_s

1check lead
Check whether the conductivity from adapter J281 to
circuit board lead P281-P411 is good and both
ends of connector are good.

2check light sensor signal


check voltage between P281plug pin 46, if the
voltage is not 5v, see (3); if voltage is 5v, check the
potential of socket pin 5 of the P281. The potential is
high when light sensor is sheltered, otherwise the
potential is low. If the potential is normal, check the
adapter board; not normal, check the light sensor

3check voltage between P282 plug pin 4


6, if the voltage is not 5v, check adapter board;
if voltage is 5v, check conductivity from adapter
board J282 to reaction disk leadP282-P037
is good and both ends of connector are
connected well.

5-6

5-7

Sample probe
abnormal.

Sample probe
abnormal.

Sample
probe fails to
leave the top
(reaction cup
side) when it
descending.
Sample
probe stays
in a valid
status of
touching or
liquid level
detecting.

The solution is as same as 5-5

MalfunctionSample probe stays in a valid status of


touching or liquid level detecting.
SolutionSample probe stops .Observe the indicator
status when raising probe(Analog touch) manually
and repeatedly and touch probe point (Analog liquid
level detection)repeatedly.
Initial statussurface_stouch_s : the touch when it
is bright; touch_s : put outliquid level detection

114

surface_s put out.


1Indicator sparkles normally after repetition of its
movement.

1check lead
Check whether the conductivity from adapter J282 to
circuit board lead P282-P037 is good and both
ends of connector are good.
(2) check sample circuit board
2Indicator not sparkle

1check whether the flexible cable of liquid


level detecting board is connected well.
2check whether the signal lead of liquid level
detection is connected well and its
conductivity is good.
3check whether the connector of liquid level
detection is connected well
4test the potential of P281 pin 8,9.
P281-8surface is high level, and on-board
indicator goes out when detecting liquid level.
P281-8surface is low level, and on-board
indicator goes out when not detecting liquid
level.
P281-9touch is high level, and on-board
indicator goes out and light sensor is sheltered if
there is any touch.
P281-9touch is low level, and on-board indicator
goes out and light sensor is not sheltered if
there is no touch.
Check adapter if the pin potential of P281 8
surface9touchis normal.

5check foot 8surface9touchof P281


are short circuit with food 7+5v, which
makes potential high.
6check whether liquid detection board works
well, if not, and please continue to check wire
line board.
Unplug the flexible cables of J6, J7 on liquid
level detection board, measure the feet J6,

115

J7-level status from right to left (J7: 1 empty,


2touch, 3surface) (J6: 1 earth, 2 empty, 3 +5 v,
4 blank)
When detecting the liquid level, J7-3surface is
high level, and on-board indicator is bight;
When not detecting the liquid level,
J7-3surface is low level, and on-board indicator
goes out;
When there is touch, the light sensor is
sheltered, and J7-2touch is high level;
When there is no touch, the light sensor is not
sheltered, and J7-2touch is low level.
Enter into system maintenance window and
execute Mechanical movement check.
Observe the running status of probe arm.
Malfunction 1probe arm stops

Solution
1, move probe mechanism, and mechanical repair is
required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.

5-8

Sample probe
abnormal.

Sample
probe fails to
reaction cup
when it
rotates to
reaction cup.

4, check the circuit board of sample is working


normally
5check the zero light sensor of up-down mechanism
is working normally
Malfunction 2Rotating arm stops at reaction cup
position.
SolutionManually make sample probe mechanism
repeatedly rise to peak, and observe swing_s
adapter indicator status.
1sparkling of swing_s indicator means the wiring of
light sensor and adaptor is normal.

1check lead
Check whether the conductivity from adapter J282 to
sample circuit board lead P282-P037 is good and
both ends of connector are good.

116

(2) check sample circuit board


2if no sparkling of swing_s indicator, check
swing_s

1check lead
Check whether the conductivity from adapter J281 to
light sensor lead P281-P411123 is good and
both ends of connector are good.

2check light sensor signal


check voltage between P281plug pin 13, if the
voltage is not 5v, see (3); if voltage is 5v, check the
potential of socket pin 2 of the P281. The potential is
high when it stops to the top of reaction cup,
otherwise the potential is low. If the potential is
normal, check the adapter board; not normal, check
the light sensor

3 check voltage between P282 plug pin 4


6, if the voltage is 5v, check adapter board; if
voltage is not 5v, check conductivity from
adapter board J282 to sample circuit board lead
P282-P037is good and both ends of
connector are connected well. If all are right,
check sample circuit board.
Malfunction 3sample probe can not reach reaction
cup
solutionMechanical repair
Enter into system maintenance window and
execute Mechanical movement check.
Observe the running status of sample probe
arm.
Malfunction 1rotating arm stops

5-9

Sample probe
abnormal.

Sample
probe fails to
leave
reaction cup
when it
moves from
reaction cup
to other
positions.

solution
as 5-8
Malfunction 2rotating arm leaves reaction cup
Malfunction
as 5-8

117

5-12

Sample probe
abnormal.

Sample
probe
deviates from
top when it
rotates.

Sample probe fails to reach the top and the


solution is as 5-1

MalfunctionProbe arm fails to descend to sample


liquid level.
solution
1, move probe mechanism, and mechanical repair is
required if resistance is big.

5-14

Sample probe
abnormal.

Sample fails
to reach
sample
liquid level.

2, check whether the flexible cable of liquid level


detecting board is connected well.
3, check whether both ends of connector of the
motor are connected well.
4 check whether the conductivity of motor lead wire
is good.
5, check liquid level detection
6, check the circuit board of sample is working
normally

9.6
Alarm
Code

Sample disk
Description

Detailed
description

Solution
Malfunction 1sample disk stops
solution

6-2

Sample disk
abnormal

Sample disk
fails to stop
at the
designated
position of
outer track.

1, rotate reagent disk, and mechanical repair is required


if resistance is big.
2, check whether both ends of connector of the motor
are connected well.
3, check whether the conductivity of motor lead wire is
good.
4, check the motor driving unit of sample disk circuit
board is working normally
Malfunction 2
When sample disk rotates to the designated position,

118

sample disk fails to stop at the designated position or


the light sensor detects it after it reaches the designated
position.
solution
Manually rotate reagent disk and observe adapter cnt_s
indicator status.
1sparkling of cnt_s indicator means the light sensor,
lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead is good and both ends
of connector are good.
2Check input part circuit of reagent disk circuit board
light sensor signal.
2if no sparkling of cnt_s, check cnt_s
1check lead
Check whether the conductivity from adapter J278 to
light sensor lead P277-P408is good and both ends
of connector are connected well.
2check light sensor signal
check voltage between P278 plug pin 79, if the voltage
is not 5v, see3; if voltage is 5v, check the potential of
socket pin 8 of the P278. The potential is high when
rotating to the zero position, otherwise the potential is
low. If the potential is normal, check the adapter board;
not normal, check the light sensor
3check voltage between P282 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is 5v,
check conductivity from adapter board J282 to reaction
disk leadP282-P048is good and both ends of
connector are connected well. If all are right, check
sample disk circuit board.

6-7

Sample disk
abnormal

Sample disk
fails to find
the zero
position.

Malfunction 1sample disk stops


solution
1, rotate reagent disk, and mechanical repair is required
if resistance is big.
2, check whether both ends of connector of the motor
are connected well.
3, check whether the conductivity of motor lead wire is
good.
4, check the motor driving unit of sample disk circuit
board is working normally

119

Malfunction 2
When sample disk rotates to the designated position,
sample disk fails to stop at the designated position or
the light sensor detects it after it reaches the designated
position.
solution
Manually rotate reagent disk and observe adapter
zero_sdisk indicator status.

1sparkling of zero_sdisk indicator means the light


sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J282 to
reaction disk circuit board lead is good and both ends of
connector are good.
2Check input part circuit of reagent disk circuit board
light sensor signal.
2if no sparkling of zero_sdisk, check zero_sdisk
1check lead
Check whether the conductivity from adapter J278 to
light sensor lead P277-P408is good and both ends
of connector are connected well.
2check light sensor signal
check voltage between P278 plug pin 13, if the voltage
is not 5v, see3; if voltage is 5v, check the potential of
socket pin 2 of the P278. The potential is high when
rotating to the zero position, otherwise the potential is
low. If the potential is normal, check the adapter board;
not normal, check the light sensor
3check voltage between P282 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J282 to
reaction disk leadP282-P048is good and both
ends of connector are connected well. If all are
right, check sample disk circuit board
Malfunctionsample fails to find sample barcode
reader.
Solution:
1, make sure that the barcode device is connected.

6-10

Sample
barcode
device
abnormal

It fails to find
sample
barcode
reader.

2 Switch R1 at the line board side and sample serial


data cable, if the bar code reader can not be found,
please check the sample disk serial circuit; if it can be
found, which means that there is no problem with the
sample disk and data cable and barcode reader may
exist problem.
3, check the bar code data cable, if the bar code
reader can not be found , which means data cable is
damaged; If can be found , which means barcode
reader is damaged and check the barcode reader.

120

9.7
Alarm
Code

Sample injection pump


Description

Detailed
description

Solution
Malfunction 1the pump stops or can not reach
the top
solution
Observe the sample pump running status:
1, When working, the sample pump is not running:
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3) check the motor drive circuit of circuit board

7-1

Sample
injection pump
abnormal

Sample
injection
pump fails to
reach the top

2When working, the sample pump is running:

(1) check whether light sensor is good


(2) Check whether the conductivity of light sensor
from adapter, reaction disk circuit board lead is
good and both ends of connector are good.
(3) check light sensor signal
check voltage between P274 plug pin 13, if the
voltage is not 5v, see3; If voltage is 5v, check the
potential of socket pin 2 of the P274. The potential is
high when the pump reaches the zero position
(discharging liquid), otherwise the potential is low. If
the potential is normal, check the adapter board; not
normal, check the light sensor
(4check voltage between P282 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J282 to reaction disk leadP282-P037
is good and both ends of connector are
connected well. If all are right, check input part

121

circuit of sample disk circuit board

Malfunction 1the pump stops or fails to leave the


top
Observe the sample pump running status:
1, When working, the motor is not running:
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3) check the motor drive circuit of circuit board
2When working, the motor is running:

(1) check whether light sensor is good

7-2

Sample
injection pump
abnormal

Sample
injection
pump fails to
leave the top

(2) Check whether the conductivity of light sensor


from adapter, reaction disk circuit board lead is
good and both ends of connector are good.
(3) check light sensor signal
3. check light sensor signal
check voltage between P274 plug pin 13, if the
voltage is not 5v, see3; If voltage is 5v, check the
potential of socket pin 2 of the P274. The potential is
high when the pump reaches the zero position
(discharging liquid), otherwise the potential is low. If
the potential is normal, check the adapter board; not
normal, check the light sensor
4. check voltage between P282 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J282 to reaction disk leadP282-P037
is good and both ends of connector are
connected well. If all are right, check input part
circuit of sample disk circuit board

122

9.8
Alarm
Code

R1 reagent probe
Description

Detailed
description

Solution
In the CS-400 upper instrument software, enter
into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the reagent probe running
status
Malfunction 1probe arm stops

solution
1, pull probe mechanism by hand, mechanical repair
is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.

8-1

R1 reagent
probe
abnormal

R1 reagent
probe fails
to reach
the top
when it
rises

4, check the motor drive module of R1 circuit board


is working normally
Malfunction 2probe arm can reach the light sensor,
but light sensor can not detect the signal
solutionManually make R1 probe mechanism
repeatedly rise to top, and observe ud_r1 adapter
indicator status.
1sparkling of ud_r1 indicator means the wiring of
light sensor, lead and adaptor is normal.
(1) check lead
Check whether the conductivity from adapter J283 to
reagent 1 circuit board is good and both ends of
connector are connected well.
(2) Check light sensor input circuit of R1 circuit board
2if no sparkling of ud_r1,check ud_r1
1check lead
Check whether the conductivity from adapter J280
to light sensor lead P282-P048is good and both
ends of connector are connected well.

123

2check light sensor signal


check voltage between P280 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P280. The potential
is high when light sensor is sheltered, otherwise the
potential is low. If the potential is normal, check the
adapter board; not normal, check the light sensor
3check voltage between P283 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J283 to
reagent 1 circuit board leadP283-P047is good and
both ends of connector are connected well. If all are
right, check reagent 1 light sensor position of circuit
board.
MalfunctionReagent probe collides with the wall or
bottom of battle when it running.
solution
Observe the reagent probe running status:
Reagent probe collides with the wall or bottom of
battle when it running
82

R1 reagent
probe
abnormal

Probe
detects level
collision

1the position of reagent probe and bottle need


to be adjusted if the probe collides with bottle wall
when running.
2If reagent probe plunges into the bottom of
battle when it is running, check whether there is
reagent in the bottle. If there is no reagent, adding
reagent to test is needed. It there is reagent,
check the circuit board of liquid level detection
and test whether the sensitivity of reagent probe is
normal.

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "Resetting", and observe the
reagent probe running status
Malfunction 1probe arm stops

8-3

R1 reagent
probe
abnormal

R1 reagent
probe fails to
leave the top
when it
descends

solution
1, pull probe mechanism by hand, mechanical repair
is required if resistance is big.
2, check whether both ends of connector of the

124

motor are connected well.


3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of R1 circuit board
is working normally
Malfunction 2probe arm can reach the light sensor,
but light sensor can not detect the signal
solutionManually make R1 probe mechanism
repeatedly rise to top, and observe ud_r1 adapter
indicator status.
1sparkling of ud_r1 indicator means the light
sensor, lead and adaptor are normal.
(1) check lead
Check whether the conductivity from adapter J283 to
reagent 1circuit board P283-P047is good and
both ends of connector are connected well.
(2) Check light sensor input circuit of R1 circuit board
2if no sparkling of ud_r1,check ud_r1
1check lead
Check whether the conductivity from adapter J280
to light sensor lead P282-P048is good and both
ends of connector are connected well.
2check light sensor signal
check voltage between P280 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P280. The potential
is high when light sensor is sheltered, otherwise the
potential is low. If the potential is normal, check the
adapter board; not normal, check the light sensor
3check voltage between P283 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J283 to reagent 1 circuit board lead
P283-P047is good and both ends of
connector are connected well. If all are right,
check reagent 1 light sensor position of circuit
board

125

Malfunction stays the valid status of collision and


liquid level detection
solutionreagent probe arm stops, and pull probe by
hand repeatedlyAnalog touchand repeatedly
touch probe topAnalog liquid level detectionand
observe adapter indicator status.
Initial statussurface_r1touch_r1: the touch when
it is bright; touch_r1 : put outliquid level detection
surface_r1: put out.
1Indicator sparkles normally after repetition of its
movement.

1check lead
Check whether the conductivity from adapter J282 to
circuit board lead P283-P047 is good and both
ends of connector are good.
(2) check sample circuit board or reagent 1

8-4

R1 reagent
probe
abnormal

R1 reagent
probe stays
the valid
status of
collision and
liquid level
detection

2Indicator not sparkle

1check whether the flexible cable of liquid


level detecting board is connected well.
2check whether the signal leadP280-P410
of liquid level detection is connected well and
its conductivity is good.
3check whether the connector of liquid level
detection is connected well
4test the potential of P280 pin 8surface
9touch
P281-8surface is high level, and on-board
indicator goes out when detecting liquid level.
P281-8surface is low level, and on-board
indicator goes out when not detecting liquid
level.
P280-9touch is high level, and on-board
indicator goes out and light sensor is sheltered if
there is any touch.
P280-9touch is low level, and on-board indicator
goes out and light sensor is not sheltered if
there is no touch.
Check adapter if the pin potential of P280 pin 8

126

surface9touchis normal.
5check foot 8surface9touchof P280
are short circuit with food 7+5v, which
makes potential high.
6check whether liquid detection board works
well, if not, and please continue to check wire
line board.
Unplug the flexible cables of J6, J7 on liquid
level detection board, measure the feet J6,
J7-level status from right to left (J7: 1 empty,
2touch, 3surface) (J6: 1 earth, 2 empty, 3 +5 v,
4 blank)
When detecting the liquid level, J7-3surface is
high level, and on-board indicator is bight;
When not detecting the liquid level,
J7-3surface is low level, and on-board indicator
goes out;
When there is touch, the light sensor is
sheltered, and J7-2touch is high level;
When there is no touch, the light sensor is not
sheltered, and J7-2touch is low level.

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the reagent probe running
status
Malfunction 1probe arm stops

8-5

R1 reagent
probe
abnormal

R1 reagent
probe can
not find the
reaction cup
when it
rotates to
reaction cup.
( reagent
probe can
not reach
zero position)

solution
1, move rotating arm mechanism by hand,
mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive circuit of reagent disk
circuit board is working normally
5, check whether up-down zero light sensor is
working normally.
Malfunction 2probe arm can reach the light sensor,

127

but light sensor can not detect the signal


solutionManually make R1 probe mechanism
repeatedly rise to the top of reaction cup, and
observe ud_r1 adapter indicator status.
1sparkling of ud_r1 indicator means the light
sensor, lead and adaptor are normal.
(1) check lead
Check whether the conductivity from adapter J283 to
reagent 1 circuit board P283-P047is good and
both ends of connector are connected well.
(2) Check R1 circuit board
2if no sparkling of swing_r1
swing_r1

indicator, check

1check lead
Check whether the conductivity from adapter J280 to
light sensor lead P280-P410123 is good and
both ends of connector are good.

2check light sensor signal


check voltage between P280 plug pin 13, if the
voltage is not 5v, see (3); if voltage is 5v, check the
potential of socket pin 2 of the P280. The potential is
high when it stops to the top of reaction cup,
otherwise the potential is low. If the potential is
normal, check the adapter board; not normal, check
the light sensor

3check voltage between P283 plug pin 46, if the


voltage is 5v, check adapter board; if voltage is not
5v, check conductivity from adapter board J282 to
reagent 1 circuit board leadP283-P047is good
and both ends of connector are connected well. If all
are right, check sample circuit board

Malfunction 3R1 reagent probe fails to leave


reaction cup
solutionmechanical repair

8-6

R1 reagent
probe
abnormal

R1 reagent
probe fails to
leave
reaction cup
when it

Enter into the "system maintenance" interface


after on-line, implement "mechanical movement
check", and observe the R1 running status
Malfunction 1reagent probe can not sway

128

rotates from
reaction cup

solutionAs 8-5
Malfunction 2rotating arm can leave reaction
cup, but light sensor can not detect the signal
solutionAs 8-5

8-7

R1 reagent
probe
abnormal

R1 reagent
probe
deflects
when
rotating

Probe fails to reach the top and solution is the


same as 8-1

MalfunctionReagent probe does not detect when


reagent bottle has liquid.
solution

88

R1 reagent
probe
abnormal

Liquid level is
not
detected at
R1 reagent
position

1check whether the wiring conductivity of liquid level


detection board and reagent circuit board is good,
and both ends of connector is connected well.
2check the circuit board of liquid level detection and
test whether the sensitivity of reagent probe is
normal
3check input circuit of reagent circuit board signal
MalfunctionR1 reagent probe fails to reach liquid
level when it descends
solution
1, move probe mechanism by hand, mechanical
repair is required if resistance is big.

8-9

R1 reagent
probe
abnormal

R1 reagent
probe fails to 2, check whether the flexible cable of liquid level
reach liquid
detecting board is connected well.
level when it
3. check whether both ends of connector of the
descends
motor are connected well.
4. check whether the conductivity of motor lead
wire is good.
5, check the liquid level detection
6. check reagent 1 circuit board

129

9.9
Alarm
Code

R2 reagent probe
Description

Detailed
description

Solution
In the CS-400 upper instrument software, enter
into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the reagent probe running
status
Malfunction 1probe arm stops

solution
1, pull probe mechanism by hand, mechanical repair
is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.

9-1

R2 reagent
probe
abnormal

R1 reagent
probe fails to
reach the top
when it rises

4, check the motor drive module of R1 circuit board


is working normally
Malfunction 2probe arm can reach the light sensor,
but light sensor can not detect the signal
solutionManually make R2 probe mechanism
repeatedly rise to top, and observe ud_r2 adapter
indicator status.
1sparkling of ud_r2 indicator means the wiring of
light sensor, lead and adaptor is normal.
(1) check lead
Check whether the conductivity from adapter J284 to
reagent 2 circuit board is good and both ends of
connector are connected well.
(2) Check light sensor input circuit of R2 circuit board
2if no sparkling of ud_ r2,check ud_ r2
1check lead
Check whether the conductivity from adapter J279
to light sensor lead is good and both ends of
connector are connected well.

130

2check light sensor signal


check voltage between P279 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P279. The potential
is high when light sensor is sheltered, otherwise the
potential is low. If the potential is normal, check the
adapter board; not normal, check the light sensor
3check voltage between P284 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J284 to reagent 2 circuit board lead
P283-P047is good and both ends of
connector are connected well. If all are right,
check reagent 2 light sensor position of circuit
board.
MalfunctionReagent probe collides with the wall or
bottom of battle when it running.
solution
Observe the reagent probe running status:
Reagent probe collides with the wall or bottom of
battle when it running
92

R2 reagent
probe
abnormal

Probe
detects level
collision

1the position of reagent probe and bottle need


to be adjusted if the probe collides with bottle wall
when running.
2If reagent probe plunges into the bottom of
battle when it is running, check whether there is
reagent in the bottle. If there is no reagent, adding
reagent to test is needed. It there is reagent,
check the circuit board of liquid level detection
and test whether the sensitivity of reagent probe is
normal.

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "Resetting", and observe the
reagent probe running status
Malfunction 1probe arm stops

9-3

R2 reagent
probe
abnormal

R2 fails to
leave the
top when
descending

solution
1, pull probe mechanism by hand, mechanical repair
is required if resistance is big.
2, check whether both ends of connector of the

131

motor are connected well.


3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of R1 circuit board
is working normally
Malfunction 2probe arm can reach the light sensor,
but light sensor can not detect the signal
solutionManually make R1 probe mechanism
repeatedly rise to top, and observe ud_ r2 adapter
indicator status.
1sparkling of ud_ r2 indicator means the light
sensor, lead and adaptor are normal.
(1) check lead
Check whether the conductivity from adapter J284 to
reagent 2 circuit board is good and both ends of
connector are connected well.
(2) Check light sensor input circuit of R1 circuit
board
2if no sparkling of ud_ r2,check ud_ r2
1check lead
Check whether the conductivity from adapter J279
to light sensor lead P280-P410 is good and
both ends of connector are connected well.
2check light sensor signal

check voltage between P279 plug pin 46, if the


voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P279. The potential
is high when light sensor is sheltered, otherwise the
potential is low. If the potential is normal, check the
adapter board; not normal, check the light sensor
3check voltage between P284 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J284 to reagent 2 circuit board lead is
good and both ends of connector are connected
well. If all are right, check reagent 2 light sensor
position of circuit board

132

Malfunction stays the valid status of collision and


liquid level detection
solutionreagent probe arm stops, and pull probe by
hand repeatedlyAnalog touchand repeatedly
touch probe topAnalog liquid level detectionand
observe adapter indicator status.
Initial statussurface_r2touch_r2: the touch when
it is bright; touch_ r2: put outliquid level detection
surface_ r2: put out.
1Indicator sparkles normally after repetition of its
movement.

1check lead
Check whether the conductivity from adapter J284 to
reagent 2 circuit board lead is good and both ends of
connector are good.
(2) check sample circuit board or reagent 2

9-4

R2 reagent
probe
abnormal

R2 reagent
probe stays
the valid
status of
collision and
liquid level
detection

2Indicator not sparkles

1check whether the flexible cable of liquid


level detecting board is connected well.
2check whether the signal lead of liquid level
detection is connected well and its
conductivity is good.
3check whether the connector of liquid level
detection is connected well
4test the potential of P279 8surface9
touch
P279-8surface is high level, and on-board
indicator goes out when detecting liquid level.
P279-8surface is low level, and on-board
indicator goes out when not detecting liquid
level.
P279-9touch is high level, and on-board
indicator goes out and light sensor is sheltered if
there is any touch.
P279-9touch is low level, and on-board indicator
goes out and light sensor is not sheltered if
there is no touch.
Check adapter if the pin potential of P279 pin 8

133

surface9touchis normal.
5check foot 8surface9touchof P280
are short circuit with food 7+5v, which
makes potential high.
6check whether liquid detection board works
well, if not, and please continue to check wire
line board.
Unplug the flexible cables of J6, J7 on liquid
level detection board, measure the feet J6,
J7-level status from right to left (J7: 1 empty,
2touch, 3surface) (J6: 1 earth, 2 empty, 3 +5 v,
4 blank)
When detecting the liquid level, J7-3surface is
high level, and on-board indicator is bright;
When not detecting the liquid level,
J7-3surface is low level, and on-board indicator
goes out;
When there is touch, the light sensor is
sheltered, and J7-2touch is high level;
When there is no touch, the light sensor is not
sheltered, and J7-2touch is low level.

In the CS-400 upper instrument software, enter


into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the reagent probe running
status
Malfunction 1probe arm stops

9-5

R2 reagent
probe
abnormal

R2 reagent
probe can
not find the
reaction cup
when it
rotates to
reaction cup.
( reagent
probe can
not reach
zero position)

solution
1, move rotating arm mechanism by hand,
mechanical repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive circuit of reagent disk
circuit board is working normally
5, check whether up-down zero light sensor is
working normally.
Malfunction 2probe arm can reach the light sensor,

134

but light sensor can not detect the signal


solutionManually make R1 probe mechanism
repeatedly rise to the top of reaction cup, and
observe ud_ r2 adapter indicator status.
1sparkling of ud_ r2 indicator means the light
sensor, lead and adaptor are normal.
(1) check lead
Check whether the conductivity from adapter J284 to
reagent 1 circuit board is good and both ends of
connector are connected well.
(2) Check R1 circuit board
2if no sparkling of swing_ r2 indicator, check
swing_ r2

1check lead
Check whether the conductivity from adapter J279 to
light sensor leads 123 is good and both ends of
connector are good.

2check light sensor signal


check voltage between P280 plug pin 13, if the
voltage is not 5v, see (3); if voltage is 5v, check the
potential of socket pin 2 of the P280. The potential is
high when it stops to the top of reaction cup,
otherwise the potential is low. If the potential is
normal, check the adapter board; not normal, check
the light sensor

3check voltage between P284 plug pin 46, if the


voltage is 5v, check adapter board; if voltage is not
5v, check conductivity from adapter board P284 to
reagent 1 circuit board lead is good and both ends of
connector are connected well. If all are right, check
sample circuit board

Malfunction 3R2 reagent probe fails to leave


reaction cup
solutionmechanical repair

9-6

R21 reagent
probe
abnormal

R2 reagent
probe fails to
leave
reaction cup
when it

Enter into the "system maintenance" interface


after on-line, implement "mechanical movement
check", and observe the reagent probe running
status

135

rotates from
reaction cup

Malfunction 1reagent probe can not sway


solutionAs 8-5
Malfunction 2rotating arm can leave reaction
cup, but light sensor can not detect the signal
solutionAs 8-5

9-7

R2 reagent
probe
abnormal

R2 reagent
probe
deflects
when rotating

Probe fails to reach the top and solution is the


same as 8-1

MalfunctionReagent probe does not detect when


reagent bottle has liquid.
solution

98

R2 reagent
probe
abnormal

Liquid level is
not detected
at R2
reagent
position

1check whether the wiring conductivity of liquid level


detection board and reagent circuit board is good,
and both ends of connector is connected well.
2check the circuit board of liquid level detection and
test whether the sensitivity of reagent probe is
normal
3check input circuit of reagent circuit board signal
MalfunctionR2 reagent probe fails to reach liquid
level when it descends
solution

9-9

R1 reagent
probe
abnormal

R2 reagent
probe fails to
reach liquid
level when it
descends

1, move probe mechanism by hand, mechanical


repair is required if resistance is big.
2, check whether the flexible cable of liquid level
detecting board is connected well.
3. check whether both ends of connector of the
motor are connected well.
4. check whether the conductivity of motor lead
wire is good.
5, check the liquid level detection
6. check reagent 2 circuit board

136

9.10

R1 reagent disk

Alarm
Code

Description

Detailed
description

10-1

No

No

Solution

No
Malfunction 1reagent disk stops
solution
1, rotate probe mechanism by hand, mechanical
repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of R1 circuit board
is working normally
Malfunction 2

10-2

R1 reagent
disk abnormal

R1 reagent
disk fails to
stop the
designated
position

When R1 reagent disk rotates to the designated


position, R1 reagent disk fails to stop at the
designated position or the light sensor detects it after
it reaches the designated position.
solution
Manually rotate reagent disk and observe adapter
cnt_r1 indicator status.

1sparkling of cnt_r1 indicator means the light


sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J283 to
reagent 1 circuit board lead is good and both ends of
connector are good.
2Check input part circuit of reagent disk circuit
board light sensor signal.
2if no sparkling of cnt_r1,check cnt_r1
1check lead

137

Check whether the conductivity from adapter J275


to light sensor lead is good and both ends of
connector are connected well.
2check light sensor signal
check voltage between P275 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 8 of the P277. The potential
is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor
3check voltage between P283 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board P283 to
reagent 1 leadP283-P047is good and both ends of
connector are connected well. If all are right, check
reagent 1 circuit board.

Enter into maintenance window and execute


Mechanical movement check, and observe its
running status.
Malfunction 1reagent disk stops

solution
1, rotate reagent disk by hand, and mechanical repair
is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.

10-3

R1 reagent
disk abnormal

R1 reagent
disk fails to
find the zero
position.

3, check whether the conductivity of motor lead wire


is good.
4, check the circuit board of reagent 1

Malfunction 2
When reagent disk rotates but can not reaches the
designated position.
solution
Manually rotate reagent disk and observe adapter
zero_r1_disk indicator status.

1sparkling zero_r1_disk indicator means the light


sensor, lead and adaptor are normal.

138

1check lead
Check whether the conductivity from adapter J283 to
reagent 1 circuit board lead is good and both ends of
connector are good.
2Check it circuit board of reagent 1
2if no sparkling of zero_r1_disk, check zero_r1_disk
1check lead
Check whether the conductivity from adapter J275
to light sensor lead P275-P405 is good and
both ends of connector are connected well.
2check light sensor signal
check voltage between P275 plug pin 13, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 2 of the P275. The potential
is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor
3check voltage between P283 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board P283 to reagent 1 leadP282-P048is
good and both ends of connector are connected
well. If all are right, check reagent 1 circuit
board
MalfunctionIt fails to find R1 reagent disk barcode
reader.
Solution:
1, make sure that the barcode device is connected.

10-4

R1 reagent
disk barcode
device
abnormal

It fails to find
R1 reagent
disk barcode
reader.

2Check whether the barcode reader is damaged


Plug R2 data cable in R1 barcode reader and
dont touch circuit board side. Please reset
barcode if R1 doesnt work. If it still doesnt
work, check barcode reader. Barcode reader
has no problem if R1 works normally, and
problem may occurs on data cable or circuit
board.
3Check data cable
Plug R2 serial cable into R1 board. Normal
working of barcode means R1 cable is
damaged and check data cable; check reagent
1 circuit board if barcode can not working
normally.

139

9.11
Alarm
Code

R2 reagent disk
Description

Detailed
description

Solution
Malfunction 1reagent disk stops
solution
1, rotate probe mechanism by hand, mechanical
repair is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
3, check whether the conductivity of motor lead wire
is good.
4, check the motor drive module of R2 circuit board
is working normally
Malfunction 2

11-2

R2 reagent
disk abnormal

R2 reagent
disk fails to
stop the
designated
position

When R2 reagent disk rotates to the designated


position, R2 reagent disk fails to stop at the
designated position or the light sensor detects it after
it reaches the designated position.
solution
Manually rotate reagent disk and observe adapter
cnt_R2 indicator status.

1sparkling of cnt_R2 indicator means the light


sensor, lead and adapter are normal.
1check lead
Check whether the conductivity from adapter J284 to
reagent 2 circuit board lead is good and both ends of
connector are good.
2Check input part circuit of reagent disk circuit
board light sensor signal.
2if no sparkling of cnt_R2,check cnt_R2

140

1check lead
Check whether the conductivity from adapter J277
to light sensor lead P277-P407 is good and
both ends of connector are connected well.
2check light sensor signal
check voltage between P277 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 8 of the P277. The potential
is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor
3check voltage between P284 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J284 to
reagent 2 lead P283-P047is good and both ends
of connector are connected well. If all are right, check
reagent 2 circuit board.

Enter into maintenance window and execute


Mechanical movement check, and observe its
running status.
Malfunction 1reagent disk stops

solution
1, rotate reagent disk by hand, and mechanical repair
is required if resistance is big.
2, check whether both ends of connector of the
motor are connected well.
11-3

R2 reagent
disk abnormal

R2 reagent
disk fails to
find the zero
position.

3, check whether the conductivity of motor lead wire


is good.
4, check the circuit board of reagent 2

Malfunction 2
When reagent disk rotates but can not reach the
designated position.
solution
Manually rotate reagent disk and observe adapter

141

zero_R2_disk indicator status.


1sparkling zero_R2_disk indicator means the light
sensor, lead and adaptor are normal.
1check lead
Check whether the conductivity from adapter J284 to
reagent 2 circuit board lead is good and both ends of
connector are good.
2Check it circuit board of reagent 2
2if no sparkling of zero_R2_disk, check
zero_R2_disk
1check lead
Check whether the conductivity from adapter J277 to
light sensor lead is good and both ends of connector
are connected well.
2check light sensor signal
check voltage between P275 plug pin 13, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 2 of the P277. The potential
is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor
3check voltage between P284 plug pin 46, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board P284 to
reagent 2 lead is good and both ends of connector
are connected well. If all are right, check reagent 2
circuit board

11-4

R2 reagent
disk abnormal

It fails to find
R2 reagent
disk barcode
reader.

MalfunctionIt fails to find R2 reagent disk barcode


reader.
Solution:
1, make sure that the barcode device is connected.
2Check whether the barcode reader is damaged
Plug R2 data cable in R1 barcode reader and
dont touch circuit board side. Please reset
barcode if R2 doesnt work. If it still doesnt
work, check barcode reader. Barcode reader
has no problem if R2 works normally, and
problem may occurs on data cable or circuit
board.
3Check data cable
Plug R1 serial cable into R2 board. Normal
working of barcode means R2 cable is
damaged and check data cable; check reagent
2 circuit board if barcode can not working
normally.

142

9.12

Alarm
Code

R1 injection pump

Description

Detailed
description

Solution
MalfunctionR1injection pump stops or fails to reach
the top
solution
observe R1 pump running status
1when working, R1 pump is not running.
(1) check whether both ends of connector of the
motor are connected well.
(2) check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working, R1 pump is running
(1) check light sensor
(2) Check whether the conductivity of light sensor
to adapter and R1 reagent disk circuit board is
good and both ends of connector are connected
well.

14-1

R1injection
pump
abnormal

R1injection
pump fails to
reach the top

3) check light sensor signal


check voltage between P274 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P274. The potential
is high when reaching to the zero
position(discharging liquid), otherwise the potential is
low. If the potential is normal, check the adapter
board; not normal, check the light sensor
4check voltage between P283 plug pin 46, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board P283 to R1 reagent circuit board lead is
good and both ends of connector are connected
well. If all are right, check the reagent input
circuit of R1 reagent circuit board.

143

MalfunctionR1injection pump stops or fails to leave


the top
solution
observe R1 pump running status
1when working, R1 pump is not running.
(1) check whether both ends of connector of the
motor are connected well.
(2) check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working, motor works normally and pump
can leave light sensor
14-2

R1injection
pump
abnormal

R1injection
pump fails to
leave the top

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and R1 reagent disk circuit board is
good and both ends of connector are connected
well.

3) check light sensor signal


check voltage between P274 plug pin 46, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 5 of the P274. The potential
is high when reaching to the zero
position(discharging liquid), otherwise the potential is
low. If the potential is normal, check the adapter
board; not normal, check the light sensor

4check voltage between P282 plug pin 46, if the


voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board P282 to R1
reagent circuit board lead is good and both ends of
connector are connected well. If all are right, check
the reagent input circuit of R1 reagent circuit board.

144

9.13
Alarm
Code

R2 injection pump
Description

Detailed
description

Solution
MalfunctionR2 injection pump stops or fails to
leave the top
solution
observe R2 pump running status
1when working, R2 pump is not running.
(1) check whether both ends of connector of the
motor are connected well.
(2) check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working, motor works normally and pump
can leave light sensor

15-1

R2 injection
pump
abnormal

R2 injection
pump fails to
reach the top

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and R2 reagent disk circuit board is
good and both ends of connector are connected
well.

3) check light sensor signal


check voltage between P274 plug pin 89, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 8 of the P274. The potential
is high when rotating to the zero position(discharging
liquid), otherwise the potential is low. If the potential
is normal, check the adapter board; not normal,
check the light sensor

4check voltage between P282 plug pin 46, if the


voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board P282 to R1
reagent circuit board lead P282-P037 is good and
both ends of connector are connected well. If all are
right, check the reagent input circuit of R2 reagent
circuit board.

145

MalfunctionR2 injection pump stops or fails to


leave the top
solution
observe R2 pump running status
1when working, R2 pump is not running.
(1) check whether both ends of connector of the
motor are connected well.
(2) check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working, motor works normally and pump
can leave light sensor

15-2

R2 injection
pump
abnormal

R2 injection
pump fails to
leave the top

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and R2 reagent disk circuit board is
good and both ends of connector are connected
well.

3) check light sensor signal


check voltage between P274 plug pin 89, if the
voltage is not 5v, see3; if voltage is 5v, check the
potential of socket pin 8 of the P274. The potential
is high when reaching to the zero position
(discharging liquid), otherwise the potential is low. If
the potential is normal, check the adapter board; not
normal, check the light sensor

4check voltage between P282 plug pin 46, if


the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board P282 to R1 reagent circuit board lead
P282-P037 is good and both ends of
connector are connected well. If all are right,
check the reagent input circuit of R2 reagent
circuit board.

146

9.14
Alarm
Code

Reaction bath
Description

Detailed
description

Solution
(1)Make sure the cooling fan is working normally

20-1

20-2

Water
temperature
of reaction
bath abnormal

Water
temperature
of reaction
bath abnormal

Water
temperature
of reaction
bath is above
45 degrees

Water
temperature
of reaction
bath is over
the rang
370.5
degrees
Note: check
only when
operating

(2) check whether temperature sensor is working


normally
(3) check whether the interface of temperature
sensor on main board
(4) replace control board or temperature sensor

(1) Make sure whether the room temperature is within


15-32 degrees.
(1)Make sure the cooling fan is working normally

(3) Make sure whether the water is circulated in


reaction bath
(4) replace control board or temperature sensor

147

9.15

0-31

0-32

ISE

Note

ISE is
over
measuring
lower
limit

Note

ISE is
over
measuring
lower
limit

ISE is over
measuring
lower limit, and
there is no
result of
current QC
ISE is over
measuring
lower limit, and
there is no
result of
current QC

1Please check whether the sample


volume, reagent volume is adequate,
and whether the position is correct

1Please check whether the sample


volume, reagent volume is adequate,
and whether the position is correct

ISE calibration

0-33

ISE is
over
Note measuring
lower
limit

is over
measuring
lower limit, and
there is no
result of
current QC

1Please check whether the sample


volume, reagent volume is adequate,
and whether the position is correct

ISE calibration

0-34

ISE is
over
Note measuring
lower
limit

is over
measuring
lower limit, and
there is no
result of
current QC

1Please check whether the sample


volume, reagent volume is adequate,
and whether the position is correct

ISE sample

0-35

Note

ISE is
over
measuring
lower
limit

is over
measuring
lower limit, and
there is no
result of
current QC

1Please check whether the sample


volume, reagent volume is adequate,
and whether the position is correct

148

19-1

37-1

38-1

39-1

60-1

Note

Electrolyte
system stops
working due to
Electrolyte the issued
alarm. Note:
function
the status of
stops
adding sample
means to
restart.

remaining
volume of
reference
ISE
referenc e liquid is
Note inadequate inadequate
(less then the
volume user
designed)

remaining
volume of
ISE
internal
internal
standard liquid
standard is inadequate
Note
liquid is
(less then the
inadequate volume user
designed)

remaining
volume of
diluent
ISE
liquid is
Note diluent is
inadequate
inadequate (less then the
volume user
designed)

Note

ISE
LEVEL
error

1Enter into system maintenance,


and execute "Resetting, and then
execute Mechanical movement
check

1add ISE reference liquid


2Enter into system maintenance,
and execute "ISE reference liquid
reagent pipeline rinsing
3Execute ISE calibration

1add ISE internal standard liquid


2Enter into system maintenance,
and execute "ISE internal standard
liquid reagent pipeline rinsing
3Execute ISE calibration

1add ISE diluent


2Enter into system maintenance,
and execute "ISE diluent reagent
pipeline rinsing
3Execute ISE calibration

In the
5-measuring
point

(1) Enter into system maintenance,


and execute "ISE check."

potential of
internal
standard
solution, the

(2) please refer to " electrolyte device


maintenance " in the "user manual" for
the detailed

149

average value
(EAV)of three
potentials is
over the
following
range

internal
standard
solutionNa
-90.0mvEAV
-10mv
In the
5-measuring
point
potential of
internal
standard
solution, the
average value
60-2

Note

ISE
LEVEL
error

(EAV)of three
potentials is
over the
following
range

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed

internal
standard
solutionK
-90.0mvEAV
-10mv
In the
5-measuring
point

60-3

Note

ISE
LEVEL
error

potential of
internal
standard
solution, the
average value
(EAV)of three
potentials is
over the
following
range

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed

internal
standard

150

solutionCl
80.0mvEAV
160mv
In the
5-measuring
point

61-1

Note

ISE
Noise
error

potential of
internal
standard
solution, the
differenceFI
of the
maximum and
minimum is
within
following
range

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed

Na
0.7mv|FIV2
-FIV4|
In the
5-measuring
point

61-2

Note

ISE
Noise
error

potential of
internal
standard
solution, the
difference
FIV of the
maximum and
minimum is
within
following
range (internal
standard,
sample)

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed

K
1.0mv|FIV2
-FIV4|

61-3

Note

ISE
Noise
error

In the
5-measuring
point
potential of
internal
standard

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed

151

solution, the
difference
FIV of the
maximum and
minimum is
within
following
range (internal
standard,
sample)
Cl0.8mv|FIV
2-FIV4|

62-1

62-2

The slope
value of
calibration
result is within
following
range or the
impact of
(1) Enter into system maintenance,
electrode is
ISE
and execute "ISE check."
Preparation low
Note abnorma l (cross-contami
nation rate (A) (2) please refer to " electrolyte device
is as following maintenance " in the "user manual" for
the detailed
Na1
32.0mvSLOPE
37mv or
68.1mvSLOPE

The slope
value of
calibration
result is within
(1) Enter into system maintenance,
following
ISE
and execute "ISE check."
Preparation range or the
Note abnorma l impact of
electrode is low (2) please refer to " electrolyte device
(cross-contami maintenance " in the "user manual" for
nation rate (A) the detailed
is as following
K1
32.0mvSLOPE
37mv
or68.1mvSLOPE

152

62-3

The slope
value of
calibration
result is within
following
range or the
impact of
ISE
electrode is
Preparation
low
Note abnorma l
(cross-contami
nation rate (A)
is as following

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed

Cl1
-30mvSLOPE
-25mv
or-68.1mvSL
OPE

63-1

Note

1The slope
value of
calibration
result is within
following
range or the
impact of
electrode is
low
ISE
(cross-contami
SLOPE
nation rate (A)
abnorma l is as following:
Na
1SLOPE
32.0mv

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed
(3) Please re-execute ISE calibration.

2current IS
calibration
result is not
updated

63-2

Note

ISE
SLOPE
abnorma l

1The slope (1) Enter into system maintenance,


value of
and execute "ISE check."
calibration
result is within
(2) please refer to " electrolyte device
following
maintenance " in the "user manual" for
the detailed
range or the

153

impact of
electrode is
low
(cross-contami
nation rate (A)
is as
following:K
1SLOPE
32mv

(3) Please re-execute ISE calibration.

2current IS
calibration
result is not
updated

63-3

Note

ISE
SLOPE
abnormal

1The slope
value of
calibration
result is within
following
range or the
impact of
electrode is
low
(cross-contami
nation rate (A)
is as
following:Cl
1SLOPE
-25.0mv

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed
(3) Please re-execute ISE calibration.

2current IS
calibration
result is not
updated

64-1

Note

1the
concentration
(1) Enter into system maintenance,
of internal
liquidCIS and execute "ISE check."
ISE the
is within
concentr following
(2) please refer to " electrolyte device
ation of
range :NaC
maintenance
" in the "user manual" for
internal
IS
the
detailed
liquid
abnorma l 120.0mmol/l or
190.0mmol/
(3) Please re-execute ISE calibration.
CIS
2current IS
calibration
result is not
updated

154

64-2

Note

1the
concentration
of internal
liquidCIS
is within
following
ISE the
concentr range .KC
IS
ation of
3.0mmol/l
or
internal

8.0mmol/
C
liquid
abnorma l IS
2current IS
calibration
result is not
updated

64-3

Note

(1) Enter into system maintenance,


and execute "ISE check."
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
the detailed
(3) Please re-execute ISE calibration.

1the
concentration
of internal
(1) Enter into system maintenance,
liquidCIS
and execute "ISE check."
is within
ISE the
following
concentr range ClC
(2) please refer to " electrolyte device
ation of
IS
maintenance " in the "user manual" for
internal
the detailed

80.0mmol/l
liquid
140.0mmol/

abnorma l
CIS
(3) Please re-execute ISE calibration.
2current IS
calibration
result is not
updated
Because of the
implementatio
n of the ISE
maintenance
(ISE cleaning,
complete
cleaning), it is
necessary to
implement ISE
calibration

Executive ISE calibration

155

Alarm
Code

Description

Detailed
description

Solution
Malfunction 1 pump stops or fails to reach the top
solution
observe pump running status
1when working, injection pump is not running.
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board

18-1

2when working,
SIP injection
pump
abnormal

SIP injection
pump fails to
reach the top

injection pump is running

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and ISE circuit board is good and both
ends of connector are connected well.

3) check light sensor signal


check voltage between P901A plug pin 13
(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P901A plug pin 23; The potential is high
when reaching to the zero position(discharging liquid),
otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 13; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

156

Alarm
Code

Description

Detailed
description

Solution
Malfunction 1

injection pump stops or fails to


leave the top

solution
observe pump running status
1when working, injection pump is not running.
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working,
18-2

SIP injection
pump
abnormal

SIP injection
pump fails to
leave the top

injection pump is running

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and ISE circuit board is good and
both ends of connector are connected well.

3) check light sensor signal


check voltage between P901A plug pin 23
(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P901A plug pin 23; The potential is high
when reaching to the zero position(discharging
liquid), otherwise the potential is low. If the potential
is normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 13; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

157

Alarm
Code
18-3

Description

Detailed
description

DIL injection
pump
abnormal

DIL injection
pump fails to
reach the top

Solution

Malfunction 1

injection pump stops or fails to


reach the top

solution
observe pump running status
1when working, injection pump is not running.
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working,

injection pump is running

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and ISE circuit board is good and both
ends of connector are connected well.
3) check light sensor signal
check voltage between P903A plug pin 13
(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P903Aplug pin 23; The potential is high
when reaching to the zero position(discharging liquid),
otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 7,9; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

158

Alarm
Code

Description

Detailed
description

Solution
Malfunction 1

18-4

DIL injection
pump
abnormal

DIL injection
pump fails to
leave the top

injection pump stops or fails to


leave the top

solution
observe pump running status
1when working, injection pump is not running.
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working,

injection pump is running

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and ISE circuit board is good and
both ends of connector are connected well.

3) check light sensor signal


check voltage between P903A plug pin 13
(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P903Aplug pin 23; The potential is high
when reaching to the zero position (discharging
liquid), otherwise the potential is low. If the potential
is normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 7,9; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

159

Alarm
Code

Description

Detailed
description

Solution
Malfunction 1 injection pump stops or fails to
reach the top

IS injection
pump
abnormal

IS injection
pump fails to
reach the top

solution
observe pump running status
1when working, injection pump is not running.
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working,

injection pump is running

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and ISE circuit board is good and both
ends of connector are connected well.

3) check light sensor signal


check voltage between P902A plug pin 13
(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P903Aplug pin 23; The potential is high
when reaching to the zero position (discharging
liquid), otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 4,6; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

160

Alarm
Code

Description

Detailed
description

Solution

Malfunction 1 injection pump stops or fails to


leave the top
solution
18-6

IS injection
pump
abnormal

IS injection
pump fails to
leave the top

observe pump running status


1when working, injection pump is not running.
(1) Check whether both ends of connector of the
motor are connected well.
(2) Check whether the conductivity of motor lead
wire is good.
(3)check motor drive circuit of circuit board
2when working,

injection pump is running

(1) check light sensor


(2) Check whether the conductivity of light sensor
to adapter and ISE circuit board is good and
both ends of connector are connected well.

3) check light sensor signal


check voltage between P902A plug pin 13
(measured when plug it into plug seat), if the voltage
is 5v, check potential (measured when plug it into
plug seat)of P902A plug pin 23; The potential is high
when reaching to the zero position (discharging
liquid), otherwise the potential is low. If the potential
is normal, Check whether the conductivity of light
sensor to adapter and ISE circuit board is good; if not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 4,6; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

161

Alarm
Code

Description

Detailed
description

Solution

Malfunction 1 Electromagnet stops or fails to


reach the bottom
solution

16-1

observe Electromagnet running status


The nozzle of
electrolyte
SIP
abnormal

SIP nozzle
fails to reach
the bottom

1when working, Electromagnet is not running.


(1) Check whether both ends of connector of the
Electromagnet are connected well.
(2) Check whether the conductivity of
Electromagnet lead wire is good.
(3)check Electromagnet drive circuit of circuit board
2when working,

Electromagnet is running

1check whether the block separates the light


sensor when the electromagnet has descended.
If not separated, adjust block till separate the
light sensor.
(2) check light sensor
(3) Check whether the conductivity of light sensor
ISE circuit board is good and both ends of
connector are connected well.
(4) check light sensor signal
check voltage (measured when plug it into plug seat)
between P904 plug pin 46, if the voltage is 5v, check
voltage (measured when plug it into plug seat)of P904
plug pin 56; The potential is high when solenoid
valve is located low, otherwise the potential is low. If
the potential is normal, Check whether the
conductivity of light sensor ISE circuit board is good; if
not normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 4,6; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

162

Alarm
Code

Description

Detailed
description

Solution
Malfunction 1 Electromagnet stops or fails to
leave the top
solution
observe Electromagnet running status

16-2

SIP injection
pump
abnormal

SIP injection
pump fails to
leave the top

1when working, Electromagnet is not running.


(1) Check whether both ends of connector of the
Electromagnet are connected well.
(2) Check whether the conductivity of
Electromagnet lead wire is good.
(3)check Electromagnet drive circuit of circuit board
2when working,

Electromagnet is running

1check whether the block left the light sensor


when the electromagnet has risen. If not left, adjust
block till left the light sensor.
(2) check light sensor
(3) Check whether the conductivity of light sensor
ISE circuit board is good and both ends of
connector are connected well.
(4) check light sensor signal
check light sensor signal
check voltage (measured when plug it into plug seat)
between P904 plug pin 46,
if the voltage is 5v, check voltage (measured when
plug it into plug seat)of P904 plug pin 56; The
potential is high when solenoid valve is located low,
otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light sensor
ISE circuit board is good; if good, check light sensor
input circuit of ISE circuit board;. If the potential is not
normal, replace the light sensor
If the voltage is not 5v, Check whether the
conductivity of light sensor ISE circuit board is
good; if good, check light sensor input circuit of
ISE circuit board

163

Alarm
Code

Description

Detailed
description

Solution

Malfunction 1 Electromagnet stops or fails to


reach the bottom

17-1

solution
The nozzle of
electrolyte SIP
abnormal

SIP nozzle
fails to reach
the bottom

observe Electromagnet running status


1when working, Electromagnet is not running.
(1) Check whether both ends of connector of the
Electromagnet are connected well.
(2) Check whether the conductivity of
Electromagnet lead wire is good.
(3)check Electromagnet drive circuit of circuit board
2when working,

Electromagnet is running

1check whether the block separates the light


sensor when the electromagnet has descended.
If not separated, adjust block till separate the
light sensor.
(2) check light sensor
(3) Check whether the conductivity of light sensor
ISE circuit board is good and both ends of
connector are connected well.
(4) check light sensor signal
check voltage (measured when plug it into plug seat)
between P904 plug pin 13, if the voltage is 5v, check
voltage (measured when plug it into plug seat)of P904
plug pin 23; The potential is high when solenoid
valve is located low, otherwise the potential is low. If
the potential is normal, Check whether the
conductivity of light sensor ISE circuit board is good; if
not normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 4,6; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

164

Malfunction 1 Electromagnet stops or fails to


leave the top
solution
observe Electromagnet running status
17-2

SIP injection
pump
abnormal

SIP injection
pump fails to
leave the top

1when working, Electromagnet is not running.


(1) Check whether both ends of connector of the
Electromagnet are connected well.
(2) Check whether the conductivity of
Electromagnet lead wire is good.
(3)check Electromagnet drive circuit of circuit board
2when working,

Electromagnet is running

1check whether the block left the light sensor


when the electromagnet has risen. If not left, adjust
block till left the light sensor.
(2) check light sensor
(3) Check whether the conductivity of light sensor
ISE circuit board is good and both ends of
connector are connected well.
(4) check light sensor signal
check light sensor signal
check voltage (measured when plug it into plug seat)
between P904 plug pin13,
if the voltage is 5v, check voltage (measured when
plug it into plug seat)of P904 plug pin23; The
potential is high when solenoid valve is located low,
otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light sensor
ISE circuit board is good; if good, check light sensor
input circuit of ISE circuit board;. If the potential is not
normal, replace the light sensor
If the voltage is not 5v, check voltage between
P190 plug pin 4,6; if voltage is 5v, replace light
sensor adapter. If the voltage is not 5v, check
input circuit of ISE circuit board.

165

9.16
Alarm
Code

Resetting and others

Description

Detailed
description

143-1

Time
synchronizat
ion failure

Sending time
synchronizati
on order
failure

143-2

Water
adding
overtime
error

143-3

143-4

143-5

143-6

143-7

143-8

Water tank
liquid system
malfunction,
water adding
overtime error

AD board
detects
malfunction
AD board
when
reset failure
resetting, AD
board reset
failure
Reaction
disk detects
malfunction
Reaction
when resetting,
disk reset
Reaction
failure
disk reset
failure
Sample disk
detects
Sample disk malfunction
when
reset
resetting,
failure
sample disk
fails to reset
R1 disk detects
malfunction
when
R1 disk
resetting, R1
reset failure
disk fails to
reset

Solution

Check the communications of control board and


sub-boards respectively.
Check whether the water supply machine, solenoid
valve, pipeline and filter are working normally, and
check whether the water supply pipe has air, and
check whether the floater is normal, and check
whether relevant electrical units of floater and control
board are normal.
Use control panel monitoring software to monitor the
fault information to see if there is AD communication
error. Repair AD board if there is AD communication
error to debug.

Lower machine monitoring software monitors error


information, and analyze the reaction disk
board-related failure to eliminate the error

Use lower machine monitoring software to monitor


error information, and refer to the related information
of sample disk board to debug.

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of R1 board to debug.

R2 disk detects
malfunction
when
Use lower machine monitoring software to monitor
resetting, R2
R2 disk
error information, and refer to the related error
reset failure disk fails to
information of R2 board to debug.
reset
Check whether the indication of reaction bath liquid
Water
Water
level detector is right; check whether there is
dischargin
dischargin
remaining water in reaction bath, and check reaction
g failure of
g failure of
bath water outlet pipeline if there is remaining water
reaction
reaction
in it; check whether relevant electrical units of liquid
bath
bath
level detection and control board are normal.

166

143-9

Adding
detergent
overtime
error

R1 and R2
reagent
probes fails
to add
detergent
completely
in the
specified
time

Connect the main control board debugging program


to electrify the machine; observe machine electrifying
flow; observe whether the 6 times the normal
cleansing movement of detergents reagent 1 reagent
2 is finished in the phase of adding detergents.
Corresponding control board of reagent probe should
be repaired if the reagent probe did not achieve
normal movement.

143-10

R1liquid
level
detection
failure

R1 fails to
detect liquid
level when
adding
detergent

Refer to liquid detection error analysis to debug

143-11

R2 liquid
level
detection
failure

R2 fails to
detect liquid
level when
adding
detergent

Refer to liquid detection error analysis to debug

143-12

Liquid level
detection
failure of
reaction
bath

Liquid level
detection
failure of
reaction bath

Check the liquid level of reaction bath, and check


whether the liquid level detector of reaction bath
works normally; check whether relevant electrical
units of liquid level detection and control board are
normal.

143-13

Reaction
bath liquid
level
malfunction

Reaction
bath liquid
level
malfunction

Check whether the liquid level detector of reaction


bath is clean, whether there is water in reaction bath,
whether water level reaches the position where the
detector can detect, and check liquid level detector in
reaction bath is working normally; check whether
relevant electrical units of liquid level detection and
control board are normal.

143-14

Bar code
scanning
overtime
error

Bar code
scanning
overtime
error

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reagent and sample disks to debug.

143-15

Pipeline
exhaust
overtime
error

Pipeline
exhaust
overtime
error

Execute the main board debugging program to


monitor, and re-execute pipeline air exhausting and
observe whether air exhausting is carried out by
reagent and sample injection pumps and the
implementation is complete. Use lower machine
monitoring software to monitor error information, and
refer to the related error information of reagent and
sample disks to debug.

143-16

Reaction
disk start
failure

Reaction
disk start
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reaction disk to debug.

167

143-17

Reaction
disk stop
failure

Reaction
disk stop
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reaction disk to debug.

143-18

Sample
probe
blocked

Sample
probe
blocked

Maintenance operation of the sample probe. Check


whether the pressure sensor and solenoid valve of
sample probe pipeline are normal; check whether
relevant electrical units of pressure detection and
control board are normal.

143-19

Previous
sample
adding
failure

Previous
sample
adding
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of sample disk to debug.

143-20

Previous
reagent 1
adding
failure

Previous
reagent 1
adding
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reagent 1 to debug.

143-21

Previous
reagent 2
adding
failure

Previous
reagent 2
adding
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reagent 2 to debug.

143-22

Previous
reagent 3
adding
failure

Previous
reagent 3
adding
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reagent 2 to debug.

143-23

Previous
reagent 4
adding
failure

Previous
reagent 4
adding
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reagent 1 to debug.

143-24

Previous
stirring 1
failure

Previous
stirring 1
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reaction disk to debug.

143-25

Previous
stirring 2
failure

Previous
stirring 2
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reaction disk to debug.

143-26

Previous
stirring 3
failure

Previous
stirring 3
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reaction disk to debug.

143-27

Previous
stirring 4
failure

Previous
stirring 4
failure

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reaction disk to debug.

143-28

Waste liquid
bottle full

Waste liquid
bottle full

Check whether waste liquid bottle is full; check


whether the sensor in waste liquid bottle is normal;
check whether relevant electrical units of the floater in

168

waste liquid bottle and control board are normal.

Switch
malfunction,
high-level
floater
detects the
signal, but
low-level
floater fails.
Reagent
level
scanning
overtime
error

Check whether the floaters of high and low liquid level


and the signal cable connecting floater to main
control board are normal; check whether relevant
electrical units of floater detection and control board
are normal.

143-29

Floater
switch error

143-30

Reagent
level
scanning
overtime
error

143-31

Vacuum
Pump
Failure

Vacuum
Pump
negative
pressure low

143-32

Sample
barcode
scanning
overtime in
testing

Sample
barcode
scanning
overtime in
testing

143-33

ISE reset
failure

ISE detects
malfunction
in resetting;
resetting
failed.

Observe whether there is ISE alarm information;


Use lower machine monitoring software to monitor
error information, and refer to the related error
information of ISE board to debug.

143-34

ISE check
maintenanc
e
movement
overtime

ISE check
maintenance
movement
overtime

Observe whether there is ISE alarm information; Use


lower machine monitoring software to monitor error
information, and refer to the related error information
of ISE board to debug.

143-35

ISE pipeline
rinsing
overtime

ISE pipeline
rinsing is
over
specified
time

Observe whether there is ISE alarm information; Use


lower machine monitoring software to monitor error
information, and refer to the related error information
of ISE board to debug.

143-36

ISE
malfunction
in testing

ISE
malfunction
when testing,
follow-up ISE
stops

Observe whether there is ISE alarm information; Use


lower machine monitoring software to monitor error
information, and refer to the related error information
of ISE board to debug.

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of reagent board to debug.
Check whether the vacuum pump and vacuum pump
switch are normal; check whether relevant electrical
units of vacuum pump switch and control board are
normal.

Use lower machine monitoring software to monitor


error information, and refer to the related error
information of sample board to debug.

169

Gear pump
failure

Gear pump
pressure low

Sending
reagent
mapping
information
failure

Sending
reagent
mapping
information
failure,
adding
reagent may
fail.

143-43

cooling
water
overtime

When the
water tank
temperature
is over 36.5
degrees, add
cold water
into the tank
into to cool
down.
Temperature
could not
drop to 35.5
degrees in
one minute,
which means
cold water
temperature
is too high.

143-44

Analyzer
module
malfunction

Malfunction
among
modules

143-45

Continuous
emergence
of dirty cups

Continuous
emergence
of 5 dirty
cups

AD
data
malfunction

AD
data
mixed

IES
malfunction
in testing

ISE
measuring
internal
standard
liquid failure

Version
number
reading
overtime

Version
number
reading
overtime

143-37

143-42

143-46

143-47

143-48

Check gear pump and pressure sensor of sample


pipeline are normal. Check whether the read value of
pressure sensor which is running is more than 2500
by using main control board debugging software;
check whether relevant electrical units of pressure
sensor and control board are normal.

Refer to instrument module error analysis

Execute the main board debugging program to


observe whether the display of temperature is normal
and room temperature is high; check the water supply
pipeline of water tank is normal; check whether
relevant electrical units of temperature sensor and
control board are normal.

Execute the main board debugging program to


monitor whether the communications of main board
and sub-boards are normal; check whether relevant
electrical units of sub-boards communications and
control board are normal.
Check whether light source, water quality of
incubation bath, reaction cup and counting light
sensor of reaction disk are normal; test data
collecting board lines by using the testing program of
data collecting board.
Execute the main board debugging program to
monitor whether the communications of main control
board and AD board is normal, and analyze error;
check whether counting light sensor is normal.
Use lower machine monitoring software to monitor
error information, and refer to the related error
information of ISE board to debug.
Execute the main board debugging program to
monitor whether the communications is normal, and
analyze error; check whether relevant electrical units
of sub-boards communications and control board are
normal.
170

9.17
Alarm
Code

Cooling system

Description

Detailed
description

Solution

(1)check whether reagent disk cover is covered,


whether the ambient temperature is in line with
environmental requirements of instrument.
144-1

Cooling system
abnormal

Cooling time
abnormal

(2) observe the temperature displayed on digital


pipe of cooling system and Peltier current value are
normal.
If abnormal: Please check Peltier and cooling
circuit board

144-2

Cooling system
abnormal

Cooling
current
abnormal

144-3

Cooling system
abnormal

Cooling chip
abnormal

144-5

Cooling
communication
abnormal

Cooling
status
abnormal

1observe whether current value displayed on


digital pipe of cooling system is normal.
2Make sure which current is abnormal, and cope
with it after check the corresponding abnormal Peltier
and circuit board.
1observe which chip is abnormal
2cope with the corresponding abnormal chip
1check the communications wiring of cooling
board and main board
2check the communications interface circuit of
cooling board
3check the communications interface circuit of
main board

The 1st line


cooling
current <5A

Check the 1st line cooling Peltier and cooling chip

145-1

The
1st
line
cooling
chip
malfunction
The 2nd line
cooling chip
malfunction

The 2nd
line cooling
current <5A

Check the 2nd line cooling Peltier and cooling chip

145-2

The 3rd line


cooling
chip
malfunction

The 3rd line


cooling
current <5A

Check the 3rd line cooling Peltier and cooling chip

145-3

The 4th line


cooling
chip
malfunction

The 4th line


cooling
current <5A

Check the 4th line cooling Peltier and cooling chip

145-4

171

9.18

Alarm
Code

AD collector

Description

Solution

The 1st line


AD collector
malfunction

The
measuring
value of the
1st line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The 2nd line


AD collector
malfunction

The
measuring
value of the
2nd line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The 3rd line


AD collector
malfunction

The
measuring
value of the
3rd line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The 4th line


AD collector
malfunction

The
measuring
value of the
4th line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The fifth line


AD collector
malfunction

The
measuring
value of the
fifth line AD
collector
is
over normal
range

Check the AD collection board and preamp board

146-1

146-2

146-3

146-4

146-5

Detailed
description

172

The sixth line


AD collector
malfunction

The
measuring
value of the
sixth line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The
measuring
value of the
seventh line
AD collector
is
over
normal range

Check the AD collection board and preamp board

146-7

The seventh
line
AD
collector
malfunction

The
measuring
value of the
eighth
line
AD collector
is
over
normal range

Check the AD collection board and preamp board

146-8

The
eighth
line
AD
collector
malfunction

The ninth line


AD collector
malfunction

The
measuring
value of the
ninth line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The
tenth
line
AD
collector
malfunction

The
measuring
value of the
tenth
line
AD collector
is
over
normal range

Check the AD collection board and preamp board

The 11th line


AD collector
malfunction

The
measuring
value of the
11th line AD
collector
is
over normal
range

Check the AD collection board and preamp board

The 12th line


AD collector
malfunction

The
measuring
value of the
12th line AD
collector
is
over normal
range

146-6

146-9

146-10

146-11

146-12

Check the AD collection board and preamp board

173