Take the sample according to type of sample (according to sample type sample volume

was given in manual)

Rinse the beaker several times with the sample.
When the sample is taking, leave some space in the beaker or conical flask. Do not take
the sample up to the neck.
Fill the burner with alcohol. And lightning the burner, heat the forceps (forceps contain
any bacteria it will die). Using the forceps take the filter paper and put in to the filtration
unit.
After putting the filter paper in filtration unit attach the pressuring kit by using the pipe.
Pore the water into filtration unit develop the pressure, water come down through filter
paper
Take the media plate add 2.5 ml of d. water to it. Take out the filter paper by using the
forceps in the presence of alcohol burner transfer in to the media.
Incubator was on before one hour and adjust the temp to 35 C. put the medias in to
incubator.
After one day or 24 48 hours interpret the results qualitatively by observing the
presence or absence for growth and qualitatively by counting the number of colonies on
the surface of the membrane filter and calculate cfu/ml