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Cells grown in culture will rapidly divide until a single layer of cells is spread over the area of the
petri dish, after which they will stop dividing. If cells are removed, those bordering the open space
will begin dividing again and continue to do so until the gap is filled - this is known as contact
inhibition. When a cell population reaches a certain density, the amount of required growth factors
and nutrients available to each cell becomes insucient to allow continued cell growth. Temperature
has a direct aect on the rate of metabolic processes. Too high and the cell cooks, too low and they
freeze. Biofilms are involved in quorum sensing, when cells are near enough to signal. Living in
biofilms makes it easier for cells to conjugate, or transfer genetic material, too. Cells in a biofilm will
produce those extracellular polysaccharide substances (EPSs) to include new cells from the outside
and other cells. If two solutions of dierent concentration are separated by a semi-permeable
membrane which is permeable to to the smaller solvent molecules but not to the larger solute
molecules, then the solvent will tend to diuse across the membrane from the less concentrated to
the more concentrated solution. This process is called osmosis. Osmosis is of great importance in
biological processes where the solvent is water. The transport of water and other molecules across
biological membranes is essential to many processes in living organisms. The energy which drives
the process is usually discussed in terms of osmotic pressure.
Structural and functional evidence supports the relatedness of all domains. Metabolic pathways
are conserved across all currently recognized domains. Structural evidence supports the relatedness
of all eukaryotes. Like the Cytoskeleton (a network of structural proteins that facilitate cell movement,
morphological integrity and organelle transport), Membrane-bound organelles (mitochondria and/or
chloroplasts), Linear chromosomes, Endomembrane systems, including the nuclear envelope.
Archaea and Bacteria generally lack internal membranes and organelles and have a cell wall.