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  • Aagguguauggcguucuggacguagaaggcaaugccuuuug: Transcription Worksheet

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    api-286011720
    24 Ansichten2 Seiten
    RNA Polymerase will help you decide where to begin polymerizing nucleotides. Record the sequence of the mRNA molecule below, starting at the +1 nuclctide. Label the 5' and 3' ends of the newly synthesized transcript.

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    key 1 transcriptionwrkst

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    RNA Polymerase will help you decide where to begin polymerizing nucleotides. Record the sequence of the mRNA molecule below, starting at the +1 nuclctide. Label the 5' and 3' ends of the newly synthesized transcript.

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    © All Rights Reserved
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    24 Ansichten2 Seiten

    Aagguguauggcguucuggacguagaaggcaaugccuuuug: Transcription Worksheet

    Hochgeladen von

    api-286011720
    RNA Polymerase will help you decide where to begin polymerizing nucleotides. Record the sequence of the mRNA molecule below, starting at the +1 nuclctide. Label the 5' and 3' ends of the newly synthesized transcript.

    Copyright:

    © All Rights Reserved
    Als DOC, PDF, TXT herunterladen oder online auf Scribd lesen
    Markieren Sie unangemessene Inhalte
    von 2

    TRANSCRIPTIONWORKSHEET

    NAME_____KEY(SEQUENCE1)___________________________________

    1.
    2.

    ChooseaDNAsequence.Recorditsnumber:______1_____
    UsingtheRNApolymerase,findthe10and35sequencesofthepromoterinyourDNAsequence.WhichDNAstrandwillbe
    thetemplatestrandfortheproductionofanmRNAmolecule(WatsonorCrick)?____WATSON____________________________

    3.

    RNAPolymerasewillhelpyoudecidewheretobeginpolymerizingnucleotides.Usthisinformationtodeterminethe+1
    nucleotideofthemRNAmolecule(thereisno0positionthebasesflanking+1are1toonesideand+2totheotherside).Record
    thesequenceofthemRNAbelow,startingatthe+1nucleotideandcontinuingthroughthe3endofthemRNA.Labelthe5and3
    endsofthenewlysynthesizedmRNAmolecule.Youdonotneedtoshowthesugarphosphatebackboneasitiscustomarytolistonly
    thesequenceofnitrogenousbases.

    5AAGGUGUAUGGCGUUCUGGACGUAGAAGGCAAUGCCUUUUG3
    3
    4.

    Ontherightarethetwodeoxyribonucleotidesof
    thetemplateDNAstrandthatarecomplementarytothe
    firsttoribonucleotidesinthemRNAtranscript.
    Completethefollowingsteps:
    a.
    Fillinthenitrogenousbases(usingA,T,
    G,orC)ofthetwodeoxyribonucleotides,paying
    closeattentiontothepolarityoftheDNA
    molecule.
    b.
    OppositetheDNAtemplate,drawthe
    firsttworibonucleotidesofthemRNA.Include
    thesugarphosphatebackboneanduseA,U,G,
    orCforthenitrogenousbases.Again,payclose
    attentiontothepolarityofthemRNAmolecule.
    c.
    Drawthehydrogenbondsbetweenthe
    basesoftheDNAtemplateandmRNA.Label
    the5and3endsofthemRNA.

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