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Series I-C

Model Paper

Basic Medical Science


Time: 80 mins

Biochemistry

Author Email: sidharta123@yahoo.com


Total Marks: 100 | Pass Mark: 60

BIOCHEMISTRY
Please use a dark pencil/pen to complete the test. Read the instructions at the
begining carefully. This is a practice test series in Biochemistry.
Please Note: There is a Negative Marking of (-1) for each incorrect response.
1. Given below is a list of Vitamins:
(i) Cholecalciferol (iii) Riboflavin (v) Niacin
(ii) Vitamin B12
(iv) Folic acid (vi) Pyridoxine
Which of the above given vitamins are involved in energy-releasing functions?
A (iii),(vi)

C (ii),(iii),(v),(vi)

B (iii),(v)

D (v),(vi)

2. Assertion (A) & Reason (R):


Assertion: Cysteine contains disulphide bonds formed from (-SH) group of two
cystine residues
Reason: Disulphide groups (-SH) confers stability and enhances solubility of the
protein
A Both A and R are true and R is
the correct explanation of A

C A is true but R is false


D Both A and R are false

B Both A and R are true and R is


not the correct explanation of A

3. Coenzyme Flavin nucleotide is derived from:


A Vit. B3

C Vit. B6

B Vit. B2

D Vit. B1

4. The enzyme 'xanthine oxidase' in phase I reaction of drug metabolism is found in


A Lysosomes

C Cytosol

B Mitochondria

D Microsomes

5. Nicotinamide is also known as:


A pyridine-3-carboxylic acid

C aminoimidazole carboxamide

B pyridine-3-carboxamide

D N'-methyl-2-pyridone-5-carboxamide

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

6. In folic acid synthesis, enzyme/substrate that is not required:


A N-methyl-6-pyridine-3
carboxamide

C folylpoly--glutamyl synthase
D 6-hydroxymethyl-7,8-dihydropterin-pyrophosphate

B P-aminobenzoic acid
7. Fill in the blanks:
Amino acid1 + E-PLP

[A]_______________ + E-PMP

8. Nicotinamide in humans is primarily methylated to generate


A Nicotinuric acid

C 5-methyl-H4PteGlun

B N'-methylnicotinamide

D N-methyl-2-pyridone-5-carboxamide

9. Sustained consumption of avidin results in deficiency of which vitamin?


A Riboflavin

C Niacin

B Biotin

D Thiamine

10. In animals, the pyridine ring of NAD can be synthesized from


A aspartic acid

C tryptophan

B dihydroacetone phosphate

D tyrosine

11. Cystathionine index may be employed to assess Vitamin B6 status: Metabolism of


which amino acid is involved in its load test?
A Tryptophan

C Cysteine

B Methionine

D Histidine

12. Condition(s) in which tryptophan metabolism is altered


A sickle cell anemia

C pregnancy

B rheumatic fever

D rheumatoid arthritis

13. Match the following amino acids according to their corresponding polarity and
charge:
A.
B.
C.
D.
E.

Proline
Valine
Glutamine
Glutamate
Arginine

Basic Medical Sciences

A.
B.
C.
D.
E.

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Charged positive (basic)


Polar uncharged
Charged negative (acidic)
Branched chain nonpolar
Nonpolar aliphatic

Series I-C

Model Paper

Biochemistry

14. Application of dithiothreitol (DTT) in determination of protein structure is used for:


A oxidation of disulphide bonds

C modification of reactive -SH


groups

B reduction of disulphide bonds

D identification of C terminal
residue

15. To fragment polypeptide chains, the reagent used at cleavage point Phe, Trp, Tyr
at N terminal is:
A Trypsin (porcine stomach)

C Chymotrypsin (bovine pancreas)

B Trypsin (bovine pancreas)

D Pepsin (porcine stomach)

16. Oxidative deamination of histidine produces


A Urocanic acid

C Anthranilic acid

B Histamine

D Kynurenic acid

17. Which of the following antibiotic causes renal injury if injected?


A Polymixin B

C Bacitracin

B Vancomycin

D Methicillin

18. The end product of tryptophan catabolism is:


A Alanine

C 3-hydroxy-anthralinate

B Kynurenine

D acetoacetate

19. Rearrange the following urea cycle intermediates according to their correct order
of formation:
A.
B.
C.
D.

Arginine
Argininosuccinate
Carbamoyl phosphate
Ornithine

E.

Citrulline

20. Which of the following amino acid is strictly ketogenic?


A Isoleucine

C Valine

B Leucine

D Tryptophan

21. Decarboxylation of histidine requires


A pyridoxamine-5'-phosphate

C pyridoxine-5'-phosphate

B pyridoxal-5'-phosphate

D histidine decarboxylase

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

22. Which of the following is not a polyamine?


A 1,4-Diaminobutane

C Spermidine

B Cadaverine

D Ornithine

23. Catalytic triad characteristic of some enzymes are found in:


A Carboxypeptidase A

C Renin

B Chymotrypsin

D Pepsin

24. Match the following 'enzymes' with their corresponding functions:


A.
B.
C.

Ornithine decarboxylase
Aromatic L-amino acid
decaboxylase
Polyamine oxidase

A. formation of histamine
B. spermine to putrescine
C. formation of L-Dopa

D.
E.

Tyrosine hydroxylase
Dopamine -oxidase

D. formation of norepinephrine
E. polyamine biosynthesis

25. Difluoromethylornithine (DFMO) is a drug used to treat which of the following


disease(s)?
A African sleeping sickness

C Kala-azar

B Yellow fever

D Dengue

26. Which of the following is a sulphur containing amino acid that contains a
disulphide bond (-S-S-)?
A Serine

C Cysteine

B Methionine

D Cystine

27. Amino acid that contains a double ring in its structure:


A Proline

C Tryptophan

B Tyrosine

D Phenylalanine

28. Cyanogen bromide (CNBr) is used to split polypeptide chains on


A C-terminal of cysteine residues

C C-terminal of arginine residues

B C-terminal of methionine residues

D C-terminal of lysine residues

29. Nanogram concentration of amino acids can be detected by using which of the
following reagents?
A Ninhydrin

C Fluorodinitrobenzene (FDNB)

B Fluorescamine

D Dansyl chloride

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Series I-C

Model Paper

Biochemistry

30. Which one of the following agents stimulates TSH secretion?


A Dopamine

C Glucocorticoids

B somatostatin

D propylthiouracil

31. The site of entry of threonine into TCA cycle for formation of glucose:
A Succinyl-CoA

C -ketoglutarate

B Pyruvate

D Oxaloacetate

32. About triiodothyronine (T3):


A is mostly bound to TBG
B free form of T3 is greater than the
free form of T4

C its biologic half-life is longer than


T4
D 80% of T3 is formed from
deiodination of T4

33. Broad spectrum antibiotics include:


A Erythromycin

C Penicillin

B Streptomycin

D Trimethoprim

34. Ureotelic animals include:


A Sharks

C reptiles

B birds

D bony fishes

35. During urea biosynthesis in humans, carbamoyl phosphate donates its carbamoyl
groups to:
A Citrulline

C Argininosuccinate

B Ornithine

D Urea

36. An uncommon amino acid residue found in collagen:


A dehydrohydroxylisinonorleucine

C 5-hydroxylysine

B 4-hydroxyproline

D all of the above

37. A vitamin that acts as a cofactor in serine metabolism:


A Riboflavin

C Thiamine

B Folic acid

D Pyridoxine

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Series I-C

Model Paper

Biochemistry

38. Which one of the following is an example of a serine protease?


A Plasminogen

C Plasmin

B 1-AT (antitrypsin)

D Tissue plasminogen activator


(TPA)

39. Drug that is given to prevent thrombosis in patients who are prone to develop clots:
A Dicoumarol

C Desmopressin

B Vitamin K

D Ethamsylate

40. An example of a serine protease inhibitor:


A Thromboplastin

C Antithrombin III

B Thrombin

D Plasmin

41. Match the following:


A.

Ehlers-Danlos syndrome

B.
C.
D.
E.

Osteogenesis imperfecta
Rotor syndrome
Alport syndrome
Stickler syndrome

A. chronic conjugated
hyperbilirubinemia
B. loose joints
C. abnormal fragility and bone formation
D. degeneration of joint cartilage
E. end-stage renal disease

42. A child diagnosed with homocystinuria has mental retardation and faulty
development of bones. The biochemical basis of this disease is due to defective
enzymeA Kynureninase

C Homogentisate 1,2 dioxygenase

B Homoserine deaminase

D Cystathionine -synthase

43. A pyridoxal phosphate enzyme:


A Tryptophan oxygenase

C Kynureninase

B -ketoacid decarboxylase

D Kynurenine formylase

44. All of the following are phenylalanine catabolites except:


A phenylacetate

C phenyllactate

B p-hydroxyphenyllactate

D phenylacetylglutamine

45. Conversion of phenylalanine to tyrosine is catalyzed by:


A phenylalanine hydroxylase

C tyrosine decarboxylase

B tyrosinase

D dopa decarboxylase

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Series I-C

Model Paper

Biochemistry

46. Vitamin D resistant rickets (VDRR) is characterized by:


A lack of parathyroid hormone
(PTH)

C nephrocalcinosis
D hyperphosphataemia

B hypophosphataemia

47. Which vitamin acts as a coenzyme for reaction that yields methionine from
homocysteine?
A Biotin

C Pyridoxine

B Thiamine

D Cobalamin

48. A drug that inhibits monoamine oxidase thus prolonging the action of serotonin:
A iproniazid

C prazosin

B imipramine

D fluoxetine

49. Which of the following compound is essential for growth and differentiation of
epithelial cells?
A Retinol

C Retinoic acid

B Retinal

D Retinyl palmitate

50. -pleated sheets that occur in some proteins is due to


A covalent bonding

C disulphide linkages

B hydrogen bonding

D proline residues

51. Not true about a protein at isoelectric pH (the pI)


A has a net charge of zero

C R-groups reflects the nature


isoelectric pH of a protein

B they do not migrate in an


electrical field

D isoelectric pH of glycine is 4.97

52. Which of the following statements about -Aminobutyric acid (GABA) is/are not
true?
A is formed by decarboxylation of
glutamate

C require pyridoxal phosphate


(PLP)

B is inhibited by drug lamotrigine

D is an excitatory neurotransmitter

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Series I-C

Model Paper

Biochemistry

53. Assertion (A) & Reason (R):


Assertion: In electrophoresis, a protein will move toward cathode if the pI is below
isoelectric point
Reason: The protein has a net negative charge and so will migrate towards the
cathode
A Both A and R are true and R is
the correct explanation of A

C A is true but R is false


D Both A and R are false

B Both A and R are true and R is


not the correct explanation of A

54. Which of the following enzyme is absent in human beings?


A aldehyde dehydrogenase

C lactate dehydrogenase

B pyruvate decarboxylase

D pyruvate carboxylase

55. A regulatory enzyme that is inhibited by NADH


A isocitrate dehydrogenase

C aldehyde dehydrogenase

B malate dehydrogenase

D D--hydroxybutyrate
dehydrogenase

56. What is not true about the pyruvate dehydrogenase complex?


A provides substrates for
gluconeogenesis

C a reversible reaction
D source of acetyl CoA for Krebs
cycle

B inhibited by acetyl CoA

57. One of the following is not an urea cycle intermediate:


A Argininosuccinate

C glutamate

B Ornithine

D Carbamoyl phosphate

58. An example of serine endopeptidase:


A Carboxypeptidase A

C Chymotrypsin

B Aminopeptidase

D Enteropeptidase

59. Regarding binding of DNA around histone, spot the incorrect statement(s):
A Unbound negative supercoil is
plectonemic

C requires compression of the


minor groove of DNA helix

B binding depends on abundance


of A=T base pairs

D topoisomerase causes relaxation


of positive supercoil

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

60. Match the following:


A.
B.
C.
D.
E.

Fibrinogen
Fibrin clot
Prothrombin
Ornithine transamination
Glutamate transamination

A.
B.
C.
D.
E.

lysine-glutamine amide bond


-carboxyglutamate
-ketoglutarate
arginine-glycine peptide bond
glutamate -semialdehyde

61. Vitamin which is a component of Acyl Carrier Protein (ACP):


A Vitamin A

C Pantothenic acid

B Thiamine

D Niacin

62. Amino acid that is unable to furnish carbon for glucose synthesis
(nonglucogenic/nonketogenic):
A Leucine

C Isoleucine

B Cysteine

D Tyrosine

63. Which one of the following functions as lubricants in synovial fluid of joints?
A Chondroitin sulphate

C Heparan sulphate

B Hyaluronic acid

D Keratan sulphate

64. Least toxic among the following:


A ammonia

C urea

B glutamine

D uric acid

65. A basic amino acid:


A Glutamate

C citrulline

B hydroxyproline

D selenocysteine

66. An imino acid that is a constituent of fibrous structure of collagen


A Lysine

C Proline

B Histidine

D Arginine

67. Given below is a list of clotting factors of the coagulation cascade.


(i) Factor I
(iii) Factor V (v) Factor VIII
(ii) Factor XI (iv) Factor II (vi) Factor XII
Which of the above given factors are activated by thrombin?
A (i),(ii),(iv)

C (iii),(v),(vi)

B (iv),(v),(vi)

D (i),(iii),(vi)

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Series I-C

Model Paper

Biochemistry

68. Consider the following statements about insulin:


Statement 1: The number of GLUT1 transporters in erythrocytes are increased by
insulin
Statement 2: Elevated insulin levels in well-fed state cause an increase in
fructose 2,6-bisphosphate
Statement 3: High insulin levels in well-fed state depresses pyruvate kinase in
liver
Statement 4: The number of GLUT4 transporters in skeletal muscle and adipose
tissue are increased by insulin
Which of the statements given above are correct?
A 2 and 4

C 3 and 4

B 1 and 3

D 2 and 3

69. Assertion (A) & Reason (R):


Assertion: Preproinsulin is cleaved in secretory granules releasing the C-peptide
that forms the active glycoprotein hormone insulin
Reason: Elevated C-peptide level is a diagnostic marker for tumors of pancreatic
-cells and NIDDM
A Both A and R are true and R is
the correct explanation of A

C A is true but R is false


D Both A and R are false

B Both A and R are true and R is


not the correct explanation of A

70. The reaction 'Glucose to Glucose-6 phosphate' in muscles is mediated by


A aldose reductase

C phosphoglucomutase

B Hexokinase

D Glucokinase

71. Pyruvate is converted to acetyl CoA in mitochondria by


A pyruvate kinase

C pyruvate dehydrogenase

B pyruvate carboxylase

D acetyl CoA carboxylase

72. -alanine is a constituent of which Vitamin?


A Retinoic acid

C Ascorbic acid

B Nicotinic acid

D Pantothenic acid

73. All of the following statements regarding triiodothyronine (T3) are incorrect except:
A deiodinase D3 is the main source
of T3 in the blood

C fasting increases the plasma T3


levels by 10-20%

B selenium deficiency causes a fall


in plasma T3 level

D T3 is the primary feedback


regulator of TSH secretion

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

74. Oxidation of iodide is mediated by:


A thyroid peroxidase

C iodinase

B iodotyrosine deiodinase

D Both a & c

75. Tyrosine is a precursor of all of the following hormones except:


A thyroxine

C epinephrine

B melanin

D serotonin

76. Which of the following is the best source of ascorbic acid (Vit.C)?
A Currant, black

C Brussels, sprout

B Rose hips

D Parsley

77. One of the following is useful in treating leukemic patients:


A aspartate

C asparagine

B asparaginase

D arginase

78. Essential amino acid(s) include:


A Threonine

C Serine

B Phenylalanine

D Tyrosine

79. Daily nitrogen excretion by human beings is about:


A 16.5 gm

C 8-25 mg

B 4.5 gm

D 7.5 mg

80. One of the following enzymes is not involved in urea biosynthesis:


A L-ornithine transcarbamoylase

C Carbamoyl phosphate synthase II

B Carbamoyl phosphate synthase I

D Argininosuccinase

81. Phosphate excretion by the kidneys is augmented (enhanced) by:


A Insulin

C Calcitonin

B Parathyroid hormone (PTH)

D 1,25-dihydroxyvitamin D

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Series I-C

Model Paper

Biochemistry

82. Assertion (A) & Reason (R):


Assertion: Folic acid deficiency can be diagnosed by FIGlu excretion test.
Reason: Accumulation of N-formiminoglutamate is due to increased catabolism of
uronic acid.
A Both A and R are true and R is
the correct explanation of A

C A is true but R is false


D Both A and R are false

B Both A and R are true and R is


not the correct explanation of A

83. One that is not a constituent of glycosaminoglycans (GAG):


A Neuraminic acid

C Uronic acid

B Amino sugars

D Hexosamines

84. Respiratory Quotient of fat and protein are respectively:


A 1.0, 0.8

C 0.7, 0.8

B 0.7, 1

D 0.8, 0.7

85. BMR is high in the following except:


A Cold climate

C Graves' disease

B Fever

D Myxedema

86. In which of the following conditions plasma potassium concentration is increased?


A metabolic alkalosis

C following insulin injection

B metabolic acidosis

D Conn's syndrome

87. Urea clearance by normal kidneys is about:


A 70 ml/min

C 125 ml/min

B 100 ml/min

D 225 ml/min

88. Net effect of Parathyroid hormone (PTH) on bone and kidney is:
A increases ECF calcium
concentration

C PTH increases renal clearance


of calcium

B PTH decreases renal clearance


of phosphate

D decreases ECF phosphate


concentration

89. A protein responsible for oxidation of iron


A Hemosiderin

C Apoferritin

B Ceruloplasmin

D Siderophyllin

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

90. Most of the filtered load of phosphate (about 80%) is reabsorbed from the:
A proximal tubule

C cortical collecting duct

B distal tubule

D Loop of Henle

91. Cerebral function is least impaired in


A Respiratory alkalosis

C Metabolic acidosis

B Respiratory acidosis

D Metabolic alkalosis

92. An example of oral phosphate binder:


A Echothiophate

C calcium carbonate

B sodium phosphate

D Diisopropyl-fluoro-phoshate

93. Vitamin D deficiency is diagnosed when plasma 25-(OH)D3 concentration is


below:
A 50 nmol/L

C 60 nmol/l

B 57 nmol/l

D 40 nmol/l

94. Regarding conversion of CO2 into HCO3- in erythrocytes catalyzed by the enzyme
carbonic anhydrase, which of the following acts as a substrate(s)?
A Sunlight

C Glucose

B H2O

D H2CO3-

95. Molecular weight of human hemoglobin is about:


A 21,600

C 68,000

B 16,890

D 64,000

96. Zinc is a prosthetic component of enzyme(s):


A carboxypeptidase A

C carbonic anhydrase

B alcohol dehydrogenase

D succinate dehydrogenase

97. Not an iron containing protein:


A lactoferrin

C hemosiderin

B transferrin

D ceruloplasmin

98. One molecule of glucose formation from pyruvate requires


A 6 ATP, 4 GTP, and 2NADH

C 4 ATP, 2 GTP, and 2 NADH

B 2 ATP, 2GTP, and 1 NADH

D 2 ATP, 2 GTP, and 1 NADH

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

99. Hypophosphatemia is seen in all of the following conditions except:


A respiratory alkalosis

C metabolic alkalosis

B malabsorption

D alcohol abuse

100. Pick the correct pathway of hemoglobin degradation from the given below:
A Heme -> hb -> biliverdin ->
bilirubin -> stercobilinogen ->
urobilinogen

C Heme -> hb -> urobilinogen ->


biliverdin -> bilirubin ->
stercobilinogen

B Hb -> heme -> biliverdin ->


bilirubin -> urobilinogen ->
stercobilinogen

D Hb -> heme -> bilirubin ->


biliverdin -> urobilinogen ->
stercobilinogen

Basic Medical Sciences

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Series I-C

Model Paper

Biochemistry

Disclaimer: Every attempt has been made to validate each and every
question/answers. Any remaining error is regretted. Author will not be held responsible
for any errors. Please consult standard textbooks given in the reference section for any
discrepancy. Questions with wrong answers may better be left unattempted/
unanswered. This is a practice MCQ series in Biochemistry available free to download
designed for the benefit of prospective PG candidates and UG medical students.
Reference Textbooks:
1. Principles of Biochemistry, Lehninger 4th Edition
2. Stryer Biochemistry, 5th Edition, Berg, Tymoczko and Stryer
3. Lippincott's Illustrated Reviews: Biochemistry, Harvey & Ferrier, 5th Edition

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