Masculanation of Tiger grouper (Epinephelus fuscoguttatus) by use Polychete

sp.
Ahmad Daud O, Kho ling Yin
FRI Tg. Demong, 22200 Besut, Terengganu

Introduction

Material and Methods

Results
Figure 1: Effect of polychete on sex changes of Tiger grouper after 3 month
feeding trial.
No. ID

Before
Experiment

After
Experiment

Polychete
A748
42C9
7D8E
C1DB
D9F0
92E9
9224
3252
3218
2B19
7D22
3E3D
329E
B6C3
831E

Sex
F
M
UK
F
F
F
M
F
F
M
UK
M
F
F
F

Sex
M
M
F
M
F
M
M
F
F
M
UK
M
M
F
F

Control
5FC0
9A2B
A4F4
6884
6AOA
5DCO
472A
E015
74C7
3A6A
6269
6AOD
A23C
9462
495C

Sex
F
F
F
F
F
F
F
F
F
F
M
M
M
M
M

Sex
F
F
F
F
F
F
F
F
F
F
M
M
M
M
M

45% female
and Unknown
status change
sex to male

Female not
change to male

Control (Trash fish only)

(Bilion)
1.76
1.71
1.73
1.54
1.03

Awal
Bulan 1
Bulan 2
Bulan 3
Bulan 4

Polychete (Trash fish +

Polyket) (Billion)
1.82
1.65
1.54
1.76
1.28

Figure 2:Effect of polychete on density of Tiger grouper sperm

Awal
Bulan 1
Bulan 2
Bulan 3
Bulan 4

Alkaline Phosphate (IU/L)

Control
Polychete
76.0 11.36
122.3 14.6
83.3 5.86
144.3 4.9
74.3 9.45
82.3 42.8
78.0 9.64
113.3 14.2
75.0 9.64
106.3 23.0

Estradiol (pg/ml)
Control
Polychete
68.3 39.8 84.1 77.8
7.92 0.42 39.3 24.1
12.4 1.1
11.8 0.0
11.8 0.0
11.8 0.0
13.5 3.0
12.1 0.5

Testosterone (ng/ml)
Control
Polychete
0.97 0.67
1.20 0.59
0.42 0.12
0.70 0.28
0.49 0.14
1.02 0.34
0.59 0.11
1.04 0.37
0.49 0.19
0.56 0.29

Figure 3: Effect of polychete on hormone alkaline phosphate, Estradiol and

Testosterone of Tiger grouper.

Conc.
((mg/100g)
Crude Fat
Saturated fatty
acid
Mono Unsaturated
(MUFA)
HUFA
(Highly
Unsaturated)
EPA
(Eicosapentaenoic
Acid)
DHA
Decosahexaenoic
Acid)
ARA
(Arachidonic Acid)

Polychete
4170

Trash
fish
90.0

Control

Polychete
Initial
1280
1330

388.21

30.57

494.3

874.46

10.75

242.9

820.60

32.07

347.5

498.81

4.26

117.72
169.48

508.5

Control

Polychete
Middle
1222.0
1334.0

Control

Polychete
Final
1240.0
1370.0

469.4

505.3

466.2

541.5

216.9

259.9

226.9

257.3

371.2

345.1

352.1

348.5

373.6

11.23

13.32

12.3

13.74

11.9

12.1

24.81

309.64

329.31

320.1

328.09

322.1

338.6

0.3

12.85

12.97

11.15

14.17

10.16

11.1

251.5

Figure 4: Effect of polychete on fatty acid composition in sperm of tiger grouper