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same but the cells chloroplast were concentrated in one place. The cell had
undergone plasmolysis.
Conclusion
1) Explain how diffusion went from an area of high concentration to an area of
low?
Diffusion works with respect to concentration gradient. The solute particles
obey Brownians motion. The particles bombard each other from where there are
concentrated to where there are least concentrated.
Decalcified Egg Osmosis
1) Using the graph of your data, describe each solution as to its tonicity.
(Hypertonic, hypotonic or isotonic to the egg). Be sure to explain how you
arrived at your conclusion. Support with data.
a) From my data, the solution with the lowest slope is the hypertonic
solution. The solution had 2.0M of glucose and the solution of 1.5M of
sugar had similarities in the way the behaved with the eggs. This data lets
us understand that water left the egg into the solution because the
solution was hypertonic and the content of the egg was hypotonic. Water
left the egg to the solution in order to maintain equilibrium and thus, the
mass of the egg was reduced. Both solutions were more concentrated
than the content of the eggs and thus, water had to leave the eggs.
b) From the graph, water and the solution with 0.5M of glucose had some
similarities with the way they behaved with the egg. The slope of the
graph tells us that there is an increase in mass of both eggs. This implies
that the eggs were hypertonic to the solution. So, the solution is hypotonic
solution. Water moved from the solution into the eggs by osmosis. Water
moved in to maintain equilibrium in the beaker.
2) Presuming the contents of the eggs were identical, what is the sugar content
of the eggs? Explain (using your data) how you came to your conclusion.
a) The sugar content of the egg placed in water was hypertonic because
water moved into the egg and increased its mass
b) The sugar content of the egg placed in 0.5M of solution is hypertonic
because its mass increased due to the movement of water into the egg.
c) The sugar content is hypotonic because the slope of the graph is negative
implying that water left the eggs into the solution.
d) The sugar content of the egg placed in 2.0M of sugar is hypotonic because
water left the egg and there was a decrease in the mass of the eggs.
3) Which solution created the most osmotic pressure?
The solution of 2.0M created the most osmotic pressure.
4) Describe how the decalcified egg acts as a semi-permeable membrane.
The shell of the eggs has been chemically removed those exposing its plasma
membrane. Plasma membranes are all semi-permeable thus, allowing certain
molecules to passively move across them.
5) To prepare 100ml 0f the following, how much glucose is needed:
a) 2.0M glucose from the molecular mass glucose which is 180g.
1802=360g
b) 1.5M glucose. 1801.5=270g
c) 0.5M glucose. 1800.5=90g