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Fluorescent Dyes:
Introduction
Introduction
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It is important to consider the advantages and drawbacks of ratiometric and non-ratiometric methods to design a calcium experiment. Other
important parameters to evaluate for choosing an indicator are its dissociation constant (K d) for calcium and/or other ions, excitation and
emission wavelength, quantum yield, buffering capacity, loading requirements, etc.
Calcium quantification experiments can be performed in a quantitative or semi-quantitative way. If quantitative experiments are performed, a
calibration is recommended.
Fluorescent Proteins
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