Beruflich Dokumente
Kultur Dokumente
Designation:NRS1221A
DepositedName:Bacillussubtilisvar.nigerSmithetal.
ProductSheet
Bacillusatrophaeus
(ATCC9372)
ProductDescription:Usedasasterilizationcontrolstrain.ThisstrainisrecommendedbyATCCforusein
thetestsdescribedinmilitaryspecificationMILS36586AandU.S.Pharmacopeia,23rdrev.,<1211>,pp.
19761981,1995whereonlythetaxonisspecified.Usedinsterilityassurance,asadryheatsterilization
control,andethyleneoxidesterilizationcontrol.
Propagation
PleasereadthisFIRST
StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section
BiosafetyLevel
1
Medium
ATCCMedium3:Nutrientagarornutrientbroth
GrowthConditions
Temperature:30C
Atmosphere:Aerobic
PropagationProcedure
1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#3broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
1.0mLpipette.Rehydratetheentirepellet.
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
4. Useseveraldropsofthesuspensiontoinoculatea#3agarslantand/orplate.
5. Incubatethetubesandplateat30Cfor24hours.
Notes
IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
FormerlyBacillussubtilis.DepositedasBacillussubtilisvar.niger.
VariationsincolonymorphologyandpigmentationoccuronNutrientAgar.Strainisblackontyrosineagar.
Selectionofcoloniesforpigmentenhancementisnotadvised.Threedifferentcolonymorphologieson#3agar
are1)orange,circular,entire,lowconvex,andglistening2)opaque,circular,entire,lowconvex,and
glistening3)white,irregular,undulate,andflat.
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
References
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
manner:Bacillusatrophaeus(ATCC9372)
BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.
ATCCWarranty
TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.
AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org
Orcontactyourlocaldistributor
Page1of2
Disclaimers
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas
beenconfirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
reasonableeffortismadetoensureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org
ProductSheet
Bacillusatrophaeus
(ATCC9372)
PleasereadthisFIRST
StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section
BiosafetyLevel
1
IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacillusatrophaeus(ATCC9372)
AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org
Orcontactyourlocaldistributor
Page2of2
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2013.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[08/02]