Beruflich Dokumente
Kultur Dokumente
Presented by
Dr. Harman
Multani
BIOSENSORS
Biosensor - biological sensor.
A biosensor
COMPONENTS OF BIOSENSOR
A biosensor
Physical change .
(ii) A Transducer part: collects
information from the biological part,
converts, amplifies and display them.
PARTS OF BIOSENSOR
FATHER OF BIOSENSORS
Professor Leland C Clark Jnr 19182005
GLUCOSE BIOSENSORS
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History Of Biosensors
amperometric enzyme
1987
1990
1992
1996
Launching of Glucocard
Current
nanotube, etc
Boehringer
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CLASSIFICATION OF BIOSENSORS
Transducer
Mode
of interaction
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Various
electrochemical
Advantages:
low detection limits - small samples volumes.
simple, low cost and in situ analysis .
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Analyte + Biosensor
CHEMICAL
Converted
Signals
Amplified
Displayed
ELECTRICAL
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MODE OF INTERACTION
Catalytic Biosensor
Biological material(enzymes)+Analyte
Modification of Analyte
Affinity Biosensor
Analyte+Biomolecule
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ESSENTIAL PROPERTIES OF
A BIOSENSOR:
(i)Specificity: specific to the analyte .
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MECHANISM OF ACTION
Biomolecule
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APPLICATIONS OF BIOSENSORS
Clinical diagnosis and biomedicine
Farm, garden and veterinary analysis
Process control: fermentation control and analysis
Food and drink production and analysis
Microbiology: bacterial and viral analysis
Pharmaceutical and drug analysis
Industrial effluent control
Pollution control and monitoring
Mining, industrial and toxic gases
Military applications
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Electrochemical
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Antibody sensors
Biosensors
based on directly
immobilizing antibodies on the surface of
an electrode for the detection of bacteria
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The
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Any
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Bacteriophage Sensors
Can
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ADVANTAGES
Rapidly
bacteria.
Can tolerate variety of harsh conditions,
such as under acidic or basic pH changes
etc..
Shows excellent specificity for target
bacteria E. coli including
Salmonella,Listeria
monocytogens,Campylobacter species.
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LECTIN BIOSENSORS
Plant or Animal Glycoproteins.
Bind selectively and reversibly with
polysaccharide components of bacterial
cell surface
Used for detection of E.coli
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MICROBIAL BIOSENSOR
A microbial
biosensor is an analytical
device that immobilizes microorganisms
onto a transducer for the detection of
target analytes.
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Bacteria
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subtilis
T. candida
P. putida
K. oxytoca
B. subtilis (heat killed)
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Serratia
marcescens
Pseudomonas sp.
P. putida
B. Licheniformis
G. suboxydans
C. tropicalis
A. niger
S. ellipsoideus
G. oxydans
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E. coli K12 0
Recombinant Moraxella
Recombinant P. putida
S. cerevisiae
T. ferrooxidans
P. fluorescents
Pseudomonas and Archromobacter
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Nanomaterials
Microorganism
Electrode
Au NPs
Sulfate-reducing
bacteria
foam Ni electrode
Fe3O4 NPs
Campylobacter jejuni
GCE
Au NPs
Salmonella Spp.
GCE
Listeria monocytogenes
Au microelectrodes
reduced graphene
sheets
Sulfate-reducing
bacteria
GCE
aluminum anodized
oxide (AAO) nanopore
membranes
E. coli O157:H7
Au electrode
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Nanomaterials
Microorganism
Electrode
alumina nanoporous
membrane
E. coli O157:H7
Platinum electrode
E. coli
magnetic nanoparticles
E. coli O157:H7
IDAM
magnetic nanoparticles
E. coli O157:H7
ITO
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