MEET THE

MICROSCOPE

EQ- How does a Compound Microscope work?

Compound Microscope
Compound microscopes are light illuminated.
The image seen with this type of microscope is
two dimensional.
This microscope is the most commonly used.
You can view individual cells, even living ones.
It has high magnification.
However, it has a low resolution
o Meaning?

EQ- How does a Compound Microscope work?

Resolution
The sharpness of an image

The image is actually made up of thousands of

tiny dots.
EQ- How does a Compound Microscope work?

Compound microscope
Has 2 convex lenses
The first lens, near the
specimen, magnifies the
object. Then a second
lens, near the eye,
further magnifies the
enlarged image
For example the first lens
is 40x magnification and
the second has 10x; Total
magnification is 400x
EQ- How does a Compound Microscope work?

Compound Microscope
Images
Paulownia Wood c.s.
200x

Frogs blood
1,000x

EQ- How does a Compound Microscope work?

Compound Microscope
Parts of the Microscope

EQ- How does a Compound Microscope work?

Eye Piece
This magnifies; where you look through to see the
image of your specimen.
They are usually 10X or 15X power.
Our microscopes have an ocular lens power of
10x.
Eye Piece

EQ- How does a Compound Microscope work?

Arm
Supports the tube and used for carrying the microscope

Ar
m

EQ- How does a Compound Microscope work?

Stage
The flat platform where you place your slides

Stag
e

EQ- How does a Compound Microscope work?

Coarse Adjustment
Knob
Moves the stage up and down

Coarse Adjustment
Knob

EQ- How does a Compound Microscope work?

Fine Adjustment Knob

This is used to fine-tune the focus of your
specimen. Use this AFTER using the coarse
adjustment knob

Fine Adjustment
Knob

EQ- How does a Compound Microscope work?

BASE
The bottom of the microscope, this is used for
support.

Bas
e

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Objective Lenses
These add to the magnification of the scope.
There are usually between 2 and 4 objective
lenses
They range from 4 to 100 times
magnification
Objective
What would this be when
Lenses
coupled with the eye piece
magnification of 10x?

EQ- How does a Compound Microscope work?

Stage Clips
These hold the slide in place
Expensive microscopes allow you to move the
stage clips (slides) with knobs

Stage
Clips

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Diaphragm
Controls the amount of light that goes through
the specimen

Diaphragm

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Light
Makes the specimen easier to see

Ligh
t

EQ- How does a Compound Microscope work?

Images
The image that you see through the eyepiece is
reversed
(flipped right to left) and inverted (flipped top to
bottom).
This means that when you move the slide away
from yourself, the image moves toward you.
Similarly, if you push the slide to the right, the
image heads off to the left.
This is a result of optics used in the lenses.
EQ- How does a Compound Microscope work?

Microscope Care

Always carry with 2 hands

Never touch the lenses with your fingers.
Do not force knobs
Keep objects clear of desk and cords
When you are finished with your "scope", rotate
the nosepiece so that it's on the low power
objective, roll the stage down to lowest level,
then replace the dust cover.

EQ- How does a Compound Microscope work?

Types of Microscopes
Compound (what we will use)
Scanning Electron Microscope (SEM way too
expensive)
Transmission Electron Microscope (TEM Also way
too expensive)

EQ- How does a Compound Microscope work?

Scanning Electron
Microscope

SEM use electron illumination.

The image is seen in 3-D.
It has high magnification and high resolution.
The specimen is coated in gold and the electrons
bounce off to give you and exterior view of the
specimen.
The pictures are in black and white.

EQ- How does a Compound Microscope work?

SEM Images
pigeon blood

cockroach antenna

EQ- How does a Compound Microscope work?

Transmission Electron
Microscope

TEM is electron illuminated.

This gives a 2-D view.
Thin slices of specimen are obtained.
The electron beams pass through
It has high magnification and
high resolution

EQ- How does a Compound Microscope work?

TEM Images
bacillus bacteria
dividing

mitochondria

EQ- How does a Compound Microscope work?

SEM and TEM

EQ- How does a Compound Microscope work?