Dihybrid Crosses

Dihybrid Cross
a 4x4 representation of crossing TWO traits
monohybrid = 1 trait w/ 2 alleles
= 1 allele gamete each for 2x2
box
dihybrid = 2 traits w/ 2 alleles EACH = 4 alleles
= 2 allele gametes each for 4x4
box
[one allele for each trait in each
gamete]

Solving a Dihybrid
Determine the genotypes of each
parent
Given to you, or you have to
solve for them
1234
Given: Parent #1 AABB
Given: Parent #2 aabb

Solving a Dihybrid
Create your dihybrid cross [4x4]
13

CODE
13
14
23
24

13
14
23
24

14

23

24

Solving a Dihybrid
Determine Gametes:

pair first allele of first trait with

first allele of second trait
13
1 234
AABB
first gamete = AB

Solving a Dihybrid
Determine Gametes:

pair first allele of first trait with

second allele of second trait
14
12 3 4
AABB
second gamete = AB

Solving a Dihybrid
Determine Gametes:

pair second allele of first trait with

first allele of second trait
23
1
234
AABB
third gamete = AB

Solving a Dihybrid
Determine Gametes:

pair second allele of first trait with

second allele of second trait
24
12 3 4
AABB
fourth gamete = AB

Solving a Dihybrid
Determine Gametes:

pair first allele of first trait with

first allele of second trait
13
aabb
first gamete = ab

Solving a Dihybrid
Determine Gametes:

pair first allele of first trait with

second allele of second trait
14
aabb
second gamete = ab

Solving a Dihybrid
Determine Gametes:

pair second allele of first trait with

first allele of second trait
23
aabb
third gamete = ab

Solving a Dihybrid
Determine Gametes:

pair second allele of first trait with

second allele of second trait
24
aabb
fourth gamete = ab

Solving a Dihybrid
AB
ab
ab
ab
ab

AB

AB

AB

Solving a Dihybrid
Fill in boxes:

AB
ab
ab
ab
ab

AaBb

AB

AB

AB

Solving a Dihybrid
Fill in boxes:
fill ACROSS each column with whatevers there

Solving a Dihybrid
Determine Genotype Ratios

record all genotypes you have

count and record number of each
genotype
AB

AB

AB

AB

AaBb

AaBb

AaBb

AaBb

AaBb: 16/16
ab
AaBb
[or 100%]

AaBb

AaBb

AaBb

AaBb

AaBb

AaBb

AaBb

AaBb

AaBb

AaBb

AaBb

ab

ab
ab

Solving a Dihybrid
Determine Phenotype Ratios

count how many of each phenotype you

have
record # of phenotypes
ABrepresented
AB
AB
AB
a
AaBb
b
purple, round: 16/16
a
AaBb
b
[or 100%]
a
AaBb
b
a
AaBb
b

AaBb AaBb AaBb

AaBb AaBb AaBb
AaBb AaBb AaBb
AaBb AaBb AaBb

Solving a Dihybrid #2
Determine the genotypes of each parent

Given to you, or you have to solve for

them
Given both parents are heterozygous for both
traits.
Given: Parent #1 QqRr
Given: Parent #2 QqRr

Solving a Dihybrid
Create your dihybrid cross [4x4]

Solving a Dihybrid
Determine Gametes:

pair first allele of first trait with

first allele of second trait
13
QqRr
first gamete = QR

Solving a Dihybrid
Determine Gametes:

pair first allele of first trait with

second allele of second trait
14
QqRr
second gamete = Qr

Solving a Dihybrid
Determine Gametes:

pair second allele of first trait with

first allele of second trait
23
QqRr
third gamete = qR

Solving a Dihybrid
Determine Gametes:

pair second allele of first trait with

second allele of second trait
24
QqRr
fourth gamete = qr

Solving a Dihybrid
Label cross w/ parent gametes above / to left

QR
QR
Qr
qR
qr

Qr

qR

qr

Solving a Dihybrid
Fill in boxes:
fill DOWN each column w/ whatevers there

QR
QR
Qr
qR
qr

Qr

qR

qr

Q R

qR

q r

Q R

qR

q r

Q R

qR

q r

Q R

qR

q r

Solving a Dihybrid
Fill in boxes:
fill ACROSS each column with whatevers there

QR
Qr
qR
qr

QR

Qr

qR

qr

QQRR
Q R

Q
QQRrr

QqRR
qR

QqRr
q r

QQRr
Q R

Q
QQrrr

QqRr
qR

Qqrr
q r

QqRR
Q R

Q
QqRrr

qqRR
qR

qqRr
q r

QqRr
Q R

Q
Qqrrr

qqRr
qR

qqrr
q r

Solving a Dihybrid
Determine Genotype Ratios

record all genotypes you have

count and record number of each
genotype
QR
Qr
qR
Q QQR QQR QqR
[not going to makeR
R
r
R
Qr QQR
you do it for this one..
QQrr QqRr
just know how to]
r
q QqR
qqR
QqRr
R
R
R
qr QqRr Qqrr qqRr

qr

QqRr
Qqrr
qqRr
qqrr

Solving a Dihybrid
Determine Phenotype Ratios

count how many of each phenotype you

have
record # of phenotypes represented
QR

Qr

qR

qr

Q QQR QQR QqR

9/16 - purple, round R
QqRr
R
r
R
3/16 - purple, wrinkled
Qr QQR

3/16 - white, round

q
1/16 - white, wrinkled

QQrr QqRr Qqrr

r
QqR
qqR
QqRr
qqRr
R
R
R
qr QqRr Qqrr qqRr qqrr